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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for Recombinant Human IL21 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysates were diluted to 1 ml with CoIP Lysis Buffer and the 3xFLAG-tagged bait was immunoprecipitated by incubation with 20 μl of anti-FLAG M2 Magnetic Beads (Sigma M8823) for 2 hrs at 4 °C on a tube rotator ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal FLAG-tagged OCT4 and various SOX TFs were generated by subcloning cDNAs into pFLAG-CMV5a mammalian expression vector (Sigma-Aldrich). For stable expression of mCherry and SOX15 in OEC-2 cells ...
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bioRxiv - Microbiology 2021Quote: ... The HS and CS samples were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) and quantified by inclusion of [13C6]aniline-tagged standard HS disaccharides (Sigma-Aldrich), as described (Lawrence et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The blot was stripped and then reprobed for FLAG-tagged receptor using anti-FLAG antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: Mammalian expression vectors encoding Flag-epitope tagged full-length Csk and eGFP-tagged Ptpn18 WT & Y/F mutant were transfected into 293T cells using 5 ug/mL PEI (Polyethylenimine, Sigma Aldrich) in Opti-MEM (GIBCO) ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear extracts from HEK 293 cells expressing either FLAG-tagged CXXC or PHD constructs were incubated with anti-FLAG M2 beads (Sigma Aldrich) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Cell Biology 2020Quote: Purification of mammalian Cavin1 was performed by Transfecting GFP-tagged Cavin1 using polyethylenimine (PEI) transfection reagent (Sigma-Aldrich Cat. No. 408727) with 1:4 w/w ratio (DNA:PEI ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation of lysates of hTERT-RPE1 cells stably expressing HA-tagged WDR60 or WDR34 using anti-HA agarose beads (Sigma-Aldrich). Lysis buffer containing 50 mM Tris/HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... cell surface HS level was examined after staining with anti-HS antibody (10E4, amsbio) at 5 μg/ml and FLAG-tagged enzymes were detected by using anti-FLAG® M2 antibody (Sigma), at 2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Plant Biology 2022Quote: ... resolved by SDS-PAGE and immunoblotted for the presence of the HA- tagged effector using α-HA antibody (H9658; Sigma-Aldrich) and α-HA-HRP (3F10 ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary substrate was horse radish peroxidase tagged anti-rabbit secondary antibody and anti-goat secondary antibody (1:2500, EMD Millipore). The bands were detected using a chemiluminescent substrate (SuperSignal West Pico ...
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bioRxiv - Biochemistry 2023Quote: Hexahistidine tagged hMDC1 BRCT (residues: 1891 – 2089) was cloned into pET47b vector and expressed in Rosetta™ 2(DE3) cell lines (Novagen). Cells in 1L Luria Broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-PA-tagged antibody conjugated with a PLUS oligonucleotide was prepared using the Duolink In Situ Probemaker PLUS (DUO92009; Sigma-Aldrich). The specimens were washed twice with wash buffer A and were subsequently incubated with Duolink ligation solution for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... or BSA (as a control) were fluorescently tagged with a 568nm label using Mix-N-Stain CF 568 kit (Millipore Sigma). Unincorporated label was removed by a size filtration spin column and incorporation of the tag was checked by SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged Af1521 macrodomain beads were produced in-house using BL21(DE3) bacteria and coupled to glutathione Sepharose 4B beads (Sigma-Aldrich), essentially as previously described ...
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bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
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bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 3×106 co-transformants was initially screened for growth on synthetic defined media (SD)-Leu-/ Trp-/ Ura-/ His- media containing 20mM of 3-amino-1,2,4-triazole (3-AT, Sigma-Aldrich). Plasmids were isolated from the potential positive colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+ -NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Microbiology 2020Quote: ... The culture media for the dividing cells (HI-10) consisted of RPMI-1640 medium + 10% heat inactivated FBS (Sigma), 100 μg/mL penicillin and 100 μg/mL streptomycin ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered through a 0.22 µm filter then applied to a 5 mL His-TrapTM HP (Sigma) column pre-equilibrated with the same buffer at a flow rate of 1.5 mL.min-1 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), incubated at room temperature for 20mins and poured into a 1-cm separation column (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Immunology 2024Quote: ... whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich). The plate was revealed with OPD/H2O2 HRP-chromogenic mix substrate and the absorbance was measured at 492 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... PHM005 wild type and recombinant strains were grown in Marine Broth (MB) Difco 2216 (Sigma-Aldrich) or Marine Agar (MA ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml).
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bioRxiv - Cell Biology 2020Quote: ... Solubilized recombinant FL-CatSper1 and ND-CatSper1 were pulled-down with anti-HA agarose (EMD Millipore) for 1 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoassay plates were coated with 2.5 μg/ml recombinant angiotensin converting enzyme-2 (ACE2, Sigma Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... or recombinant leptin (1 mg/kg/d dissolved in dimethyl-sulfoxide) (L 3772 Sigma Chemical Co.), or mdivi-1 (50 mg/kg/d dissolved in dimethyl-sulfoxide ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant PRDX6 was purified by gel filtration chromatography on a Superdex 200 16/600 column (Sigma) equilibrated with 20mM HEPES at pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was obtained from Sigma-Aldrich. Recombinant cathepsin B and cathepsin L were purchased from R&D Systems.
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bioRxiv - Cell Biology 2021Quote: ... The recombinant hormone was dissolved in sterile Lebovitz medium (L-15) (Sigma-Aldrich, St. Louis, USA) at the concentration of 100 µg/mL ...