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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 0.05% 3-3′ diaminobenzidine tetrahydrochloride hydrate (DAB, Sigma D5637) and 4% sucrose in PBS ...
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bioRxiv - Microbiology 2020Quote: ... the Milliplex® Map Non-Human Primate Cytokine Magnetic Bead Panel Kit (EMD Millipore Corporation, Billerica, MA) was used ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Cancer Biology 2020Quote: ... On this plasmid a single primer PCR with primer 5’-GCA AGC CCT GAA AGC GCA AG-3’ and the cDNA human HIF-1α in a p3XFLAG-CMV™-10 expression vector (Sigma, St Louis, MO, USA) (Gort et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... containing protease and phosphatase inhibitors and 100 U/mL benzonase (Sigma). Proteins were extracted from the pellets by sonication ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 µM alkaline phosphatase in HEPES buffer (pH 7; Sigma-Aldrich) was added to a mica surface and visualized ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by addition of phosphatase inhibitor cocktail (50 x, Merck Millipore). Consequently ...
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bioRxiv - Cancer Biology 2021Quote: ... protease and phosphatase inhibitor cocktails (Sigma, P-8340 and P-0044) and 30μM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cell Biology 2019Quote: ... and included protein phosphatase inhibitor cocktails I and III (Sigma-Aldrich) in cell extracts ...
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bioRxiv - Cell Biology 2019Quote: ... 1mM DTT and 1mM EDTA) containing protease and phosphatase inhibitors (Sigma) for 30 min at 4° C ...
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bioRxiv - Cell Biology 2020Quote: ... 50 mM Tris pH7.5 supplemented with PMSF and phosphatase inhibitors (Sigma). After centrifugation at 12,000 x g for 5 mins ...
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bioRxiv - Developmental Biology 2021Quote: ... and 10% glycerol) supplemented with protease and phosphatase inhibitors (Sigma-Aldrich) as previously described (3) ...
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bioRxiv - Developmental Biology 2020Quote: ... Plates were then stained for alkaline phosphatase (Sigma, 86 R-1KT) and rescanned with transmitted light ...
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bioRxiv - Cancer Biology 2022Quote: ... and a phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA), for 3 h at 4 °C on a rotation wheel ...
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bioRxiv - Neuroscience 2021Quote: ... with protease/phosphatase inhibitors and benzonase (1:500 dilution; Sigma E1014). Samples were diluted 1:250 and loaded on the 96 well FTL ElISA plate (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... and alkaline phosphatase conjugated anti-rabbit antibodies with FastBlue (Sigma Aldrich) or alkaline phosphatase coupled anti-mouse antibodies with FastBlue (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... or alkaline phosphatase coupled anti-mouse antibodies with FastBlue (Sigma Aldrich) were used for immunohistochemistry ...
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bioRxiv - Developmental Biology 2019Quote: ... Tartrate-resistant acid phosphatase (TRAP) (Sigma, Oakville, Ontario, Canada, #387-A) according to manufacturer’s instructions with minor changes (60 minute Triton-X incubation) ...
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bioRxiv - Plant Biology 2019Quote: ... 5mM DTT) with protease and phosphatase inhibitors (Sigma Aldrich and Serva). Microsomal fraction was separated as in Kierszniowska et al. ...
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bioRxiv - Genomics 2019Quote: ... cymostatin and phosphatase inhibitors (type I and type II, Sigma-Aldrich). Lysates were centrifuged at 20K × g for 15 minutes at 4°C and clear extract was collected and stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... and phosphatase inhibitor cocktail I and II 100X (Sigma, #P5726, #P0044). Samples were then incubated in ice for 30 minutes with the lysis buffer and the cell debris separated by centrifugation at 17968 x g for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... protease inhibitors and protein phosphatase inhibitors I and II (Sigma-Aldrich). After an incubation of 30 min on ice and centrifugation at 10 000g for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Triton X-100 0.5% supplemented with phosphatase and protease inhibitors (Sigma). Protein quantification was performed with BCA Protein Assay Kit (Thermo Scientific-Pierce) ...
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bioRxiv - Immunology 2019Quote: ... followed by 20 min incubation with streptavidin–phosphatase alkaline (Sigma-Aldrich) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.05% NP40 supplemented with a premade protease/phosphatase inhibitor cocktail (Sigma) including AEBSF ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-mouse IgG conjugated with alkaline phosphatase (A3688; Sigma-Aldrich). The specific protein bands were captured by Olympus μ-7000 digital camera and quantified by the image analysis software ...
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bioRxiv - Immunology 2019Quote: ... followed by an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma). Based on the ELISA readings ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with 100 μL alkaline phosphatase substrate solution (Sigma) for 2 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... pH 7.6) containing ROCHE protease cocktail phosphatase inhibitors (Sigma Aldrich 4693159001) at the manufacturer’s recommended concentrations ...
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bioRxiv - Bioengineering 2020Quote: ... 1:200 dilution Phosphatase Inhibitor Cocktail Set III (EMD Millipore # 524627). Lysate were collected and centrifuged at 4°C for 10 min at 15,000×g ...
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bioRxiv - Neuroscience 2021Quote: ... tissue-nonspecific alkaline phosphatase inhibitor (TNAPi 100 μM; Millipore, Temecula, CA), or TNF-α and IFN-γ (10 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... osteogenic differentiation was revealed by detecting alkaline phosphatase activity (Sigma B5655) after 14 days of induction ...
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bioRxiv - Microbiology 2021Quote: ... monoclonal anti-FLAG M2-alkaline phosphatase antibody (1:3000; Sigma, A9469), monoclonal mouse anti-EF-Tu antibody (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Roche anti-DIG-alkaline phosphatase Fab fragment (Millipore Sigma cat# 11093274910) were added to the blocking buffer at 1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... and phosSTOP phosphatase inhibitors (#4906845001, Sigma-Aldrich, St. Louis, MO, USA) were added to the cells that were immediately scraped down ...
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bioRxiv - Cell Biology 2022Quote: ... The protease and phosphatase inhibitor cocktails were purchased from Sigma-Aldrich.
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bioRxiv - Bioengineering 2022Quote: RIPA cell lysis buffer supplemented with protease and phosphatase inhibitors (Sigma) was used for the extraction of total protein from either brain or vascular channel ...
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bioRxiv - Immunology 2022Quote: ... and phosphatase inhibitors (P5726-1ML and P0044-1ML from Millipore Sigma). The lysates were run on a Western blot and probed for Perforin (Cell Signaling Technology #3693) ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies include alkaline phosphatase conjugated anti-Flag (Sigma-Aldrich A9469) and anti-HA antibodies (Immunoreagents #MuxOt-111-DALP) ...
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Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... 1% Triton-X containing 1x phosphatase inhibitor (EMD Millipore, Burlington, MA) and 1x protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... in TBS + 1% Triton-X100 + Protease and Phosphatase Inhibitor Cocktail (Sigma). Protein concentration was measured and adjusted to 100 mg/ml before adding the samples to 96-well plate-based multiplex assay plate (provided by Meso Scale Discovery Kit ...
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bioRxiv - Cell Biology 2019Quote: ... with added cocktail of protease and phosphatase inhibitors (MS-SAFE, Sigma). Protein concentration of cell lysates were determined using the Protein Assay Reagent (#23227 ...
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bioRxiv - Neuroscience 2019Quote: ... and a phosphatase and protease inhibitor cocktail (Sigma-Aldrich, Cat# MSSAFE). Protein concentration was determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1:100 phosphatase inhibitor cocktails (P5726 Sigma and P0044 Sigma). Protein concentrations were quantified by BCA assay (catalog ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1:100 phosphatase inhibitor cocktails (P5726 Sigma and P0044 Sigma). Protein concentrations were quantified by BCA assay (catalog ...