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Citations for Millipore Sigma :
1601 - 1650 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... followed by a blocking and permeabilization step in 1% bovine serum albumin (Sigma, A9647-50G) in PBST (0.2% Triton X-100 in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... pH=8) and incubated for 1 h in a Western Blocking Reagent solution (Sigma-Aldrich). Membranes were incubated overnight at 4 °C with 20 ml of the respective primary antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were pre-incubated with a blocking buffer (0.5% Triton X-100 (Sigma, T8787) and 10% normal horse serum (NHS ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with rabbit anti-GAD67 (1:1000, dissolved in blocking solution; Merck Millipore SAB4300642) primary antibody for 16 h overnight ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with Odyssey blocking buffer containing primary antibody and 0.05% Tween 20 (Sigma-Aldrich #P9416) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... before blocking with 300 µl/well of 0.5% bovine serum albumin (BSA; Sigma-Aldrich, UK) diluted in PBS (filtered with MF-Millipore® Membrane Filter ...
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bioRxiv - Molecular Biology 2024Quote: ... The nephrocytes were blocked with blocking buffer [50 mM Tris-Cl (pH 7.4) (Sigma-Alrich), 0.1% Triton X-100 (Sigma) ...
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bioRxiv - Physiology 2024Quote: ... and treated with a blocking solution (2% bovine serum albumin in PBT; Sigma-Aldrich #A9647) for 1 hr at 25–27 °C or overnight at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated with blocking and permeabilization solution (PBS containing 0.5 % Triton-100X (Sigma-Aldrich) and 3 % bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were diluted in blocking solution supplemented with 0.1% Tween-20 (Sigma Aldrich 274348) and incubated with tissues overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were also stained with 150 µL/well FLAG (Sigma, 1:500 in blocking buffer) primary antibody and 488 (FLAG ...
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bioRxiv - Immunology 2023Quote: ... Following incubation with anti-IL2 blocking antibody some cells were restimulated with rhIL-2 (Sigma) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... blocked by incubating in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2022Quote: ... Wells were blocked with blocking buffer—final concentration 0.1% bovine serum albumin (Milipore Sigma A7030), 0.2% Triton-X (Fisher Scientific BP151-100) ...
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bioRxiv - Genetics 2023Quote: ... cells were blocked in blocking buffer (1% w/v bovine serum albumin (Sigma-Aldrich, A9647), 0.1% v/v Tween-20 in DPBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently blocked with blocking buffer [PBST + 1% bovine serum albumin (BSA; Millipore Sigma) + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
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bioRxiv - Neuroscience 2023Quote: Cryosectioned tissues were incubated in blocking buffer (0.1% Triton X-100 (Sigma-Aldrich, T8787-100ML) + 10% normal horse serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS and incubated with blocking solution (5% donkey serum; Sigma-Aldrich) diluted in TBS with 0.25% tween (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... EPSCs were isolated by blocking chloride channels with the addition of picrotoxin (75uM; Sigma-Aldrich) into the recording ACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated with blocking buffer (TBS with 0.1% Tween-20 (Sigma-Aldrich, T8787-50ML), 0.5% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: ... After blocking the membranes were incubated overnight with desired ADA1 primary antibody (Sigma Aldrich, HPA001399) and then incubate 1 hour with secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... The blocking agent 6-Mercapto-1-hexanol and Phosphate-Buffered Saline (PBS) (Sigma-Aldrich Canada) (10.14 mM Na2PO4 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated for 2 h at RT in casein blocking buffer (Sigma Aldrich), then overnight at +4°C with rabbit anti-CtBP2 antibody (Abcam #ab128871 ...
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bioRxiv - Bioengineering 2023Quote: ... Grids were washed with PBS and blocked with blocking buffer (0.5% fish skin gelatin (Sigma) and 0.1% BSA-c (Aurion ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by blocking for 1 hour in 5% bovine serum albumin (Sigma, St. Louis, MO) diluted in PBS at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then permeabilised with a blocking solution containing 2% donkey serum (D9663, Sigma-Aldrich) and 0.2% Triton-X100 (X-100 ...
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bioRxiv - Developmental Biology 2024Quote: ... pH 7.4) at room temperature and blocked with iFLASH blocking buffer [10% FBS (Sigma, 122262), 5% DMSO (Chem Cruz ...
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bioRxiv - Biophysics 2021Quote: ... Peptide stock solutions were filtered using PVDF 0.22 µm syringe filters (Millipore Milex-HV) before use.
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bioRxiv - Cell Biology 2020Quote: ... an aliquot containing ~ 100 μg of peptides were resuspended in 133 mM HEPES (SIGMA) pH 8.5 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were eluted by adding 100 µl of 200 µg/ml 3xFLAG peptide (Sigma) in TBS and incubating with rotation at 4°C for 30 min.
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bioRxiv - Molecular Biology 2020Quote: ... C-18 ZipTip columns for concentrating and desalting the peptides were purchased from Millipore, Billerica ...
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bioRxiv - Immunology 2021Quote: ... MOG35-55 peptide (WatsonBio Sciences) was emulsified in Complete Freund’s Adjuvant (Sigma-Aldrich F5881) and given as one-time subcutaneous (s.c. ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-EGFP tagged ATP11C was eluted with 0.2 mg/ml Flag peptide (Sigma-Aldrich) in the wash buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Beads were resuspended in 120 μL 2 mg/ml 3X Flag Peptide (Millipore F4799) in Proteinase K buffer and eluted for 30 minutes at 37° C on a thermomixer at 850 RPM ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg/mL of the peptides were solubilized in 200 μL HFIP (Sigma-Aldrich), vortexed for 1 min and sonicated in 50/60 Hz bath sonicator for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... and peptides were solid-phase-extracted using Sep-Pak Light cartridges (Millipore, Billerica, MA). The peptides were separated by SCX chromatography and were analyzed on a Thermo LCQ Classic instrument (Thermo Electron ...
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bioRxiv - Biochemistry 2020Quote: ... Mass inaccuracies were corrected using ProteoMass Peptide and Protein MALDI-MS calibration standard (Sigma). Glycan compositions were manually inspected using MALDI LIFT-TOF/TOF MS/MS.
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were extracted in 70% ethanol plus 5% formic acid (Merck-Millipore) twice for 20 min with permanent shaking ...
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bioRxiv - Microbiology 2021Quote: ... the Flag- or HA-tagged proteins were eluted with Flag peptide (Sigma, cat# F4799) or HA peptide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was eluted with 100 μg/ml of 3X FLAG peptide (Sigma-Aldrich) in buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were eluted with 200 μg/ml of 3xFLAG peptide (Millipore Sigma cat #F4799) in TBS supplemented with 10 mM CaCl2 ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 mg of lyophilized CTD peptides were dissolved in 30 µl DMSO (Sigma-Aldrich). One molar equivalent of Atto-488 NHS ester (Atto-tec ...
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bioRxiv - Molecular Biology 2022Quote: ... washed in Lysis buffer with 225mM salt and Eluted with 3X Flag peptide (Sigma). The eluted fraction was loaded on 4-15% gradient gels with Input and immunoblotted with appropriate antibodies.
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bioRxiv - Neuroscience 2019Quote: ... The peptides were eluted into collection tubes and acidified with formic acid (Sigma Aldrich). Afterwards ...
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bioRxiv - Plant Biology 2021Quote: ... The peptide samples were desalted and purified with C18 ZipTip (Millipore ZipTips, micro-C18). The 1.0 μl of each sample was loaded on MALDI plate with 1.0 μl of the matrix ...
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bioRxiv - Molecular Biology 2020Quote: ... or from anti-Flag resin with buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 column preequilibrated in Amylose/Flag buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides are acidified with 10 μl 10 % trifluoroacetic acid (TFA, Sigma-Aldrich, T6508). One Evotip (Evosep ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthesized peptides were dissolved in ultrapure water (Milli-Q system, Millipore, Bedford, MA), store at 4°C for later use.