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Citations for Millipore Sigma :
1601 - 1650 of 2423 citations for RhoD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 1X PBS followed by permeabilization and blocking using 0.1% Triton X-100 (Sigma, T8787) and 2% Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated with blocking solution (0.5% Triton X, 2.5% bovine serum albumin [Sigma-Aldrich], 2.5% donkey serum [Sigma-Aldrich]) for one day at 37 °C before being incubated with the primary antibody at 1:500 for 3 days at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse 1:10,000 or anti-rabbit 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... blocking solution was removed and the sample was incubated in anti-fibronectin I antibody from rabbit (Sigma-Aldrich, #F3648), diluted 1:500 in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections for immunohistological staining were incubated in a blocking solution consisting of 10% Bovine Serum Albumin (BSA, Sigma-Aldrich) in PBST (1X PBS + 0.3% Triton-X100 (Roth)) ...
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bioRxiv - Bioengineering 2023Quote: ... slides were incubated in a blocking solution containing 0.3% Triton X-100 and 10% normal donkey serum (EMD Millipore) in 1xPBS + 100 mM CaCl2 + 100 mM MgCl for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG and AgRP primary antibodies were used and diluted in blocking buffer at 1:200 (mouse anti-FLAG, Sigma) and 1:1000 (rabbit anti-AgRP ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed before blocking in PBS +0.01% Triton X-100 and 0.2% fish skin gelatin (Sigma-Aldrich - G7765) for 1 h at RT ...
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bioRxiv - Biophysics 2024Quote: ... blocking solution was removed and cells were incubated with the APP/Aβ-specific primary antibody WO2 (#MABN10, Sigma Aldrich) at a 1:500 dilution for 16h at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... cells in suspension were incubated for 20 minutes at 37°c in the blocking antibody (integrin α5β1, clone JBS5, Sigma) or control antibody (IgG ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed once with PBS and treated with a permeabilization/blocking solution containing 0.1% Triton X-100 (Sigma) and 5% Normal Donkey Serum (NDS ...
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bioRxiv - Cell Biology 2020Quote: ... was aspirated and the beads were then washed three times with TBS buffer supplemented with 0,1% digitonin and eluted by competition with 150μg/ml 3xFLAG peptide (Sigma) in TBS + 0,1% digitonin for 30 minutes at 4°C in an end over end shaker.
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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bioRxiv - Immunology 2021Quote: ... The detached peptides were separated from HLA molecules using 3 kDa cut-off centrifugal ultrafilters (Millipore, MO, USA), and then concentrated using vacuum centrifugation.
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bioRxiv - Molecular Biology 2019Quote: ... beads were incubated with 0.2 bed volumes of Elution Buffer containing 5 mg/mL FLAG peptide (Sigma-Aldrich) for 10 min at 4°C and subsequently 0.6 bed volumes of Elution Buffer with PI were added and incubated with end-over-end rotation for 2 h at 4°C ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... 0.1% NP-40) and eluted in 43 µl of a 200 mg/ml dilution of FLAG peptides (Sigma) in 1x TBS ...
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bioRxiv - Immunology 2019Quote: ... Then,15 μg of peptides were purified using ZipTipC18 pipette tips according to manufacturer’s instructions (Millipore, Billerica, USA), resuspended in 2% Acetonitrile/0.1% formic acid solution ...
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bioRxiv - Biophysics 2019Quote: ... Peptide mixtures were separated using a gradient from 92% A (0.1% formic acid, FA, Sigma, in deionized water) and 8% B (0.1% FA in acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were eluted by adding 125 ng/ml FLAG peptide to the OG-containing buffer (Sigma-Aldrich) and incubating the beads for 30 min per round of elution ...
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bioRxiv - Cell Biology 2019Quote: ... Immune complexes were washed two times with lysis buffer and eluted with 0.5 mg/mL Flag peptide (Sigma) in lysis buffer ...
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bioRxiv - Bioengineering 2019Quote: ... The PEG-nanoparticle-peptide mixture was added to 15 mL of mineral oil (Sigma Aldrich, St. Louis, USA) with 1% v/v surfactant (Span 80/Tween 80 ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Biophysics 2020Quote: ... Elution was performed for 20 min at room temperature with TBS buffer supplemented with 3xFLAG peptide (Sigma-Aldrich) at a final concentration of 0.15 μg/ml.
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Microbiology 2021Quote: ... The beads were washed with 1x Tris-buffered saline (TBS) three times and proteins were eluted with 55μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x TBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted twice with equal bead volume (200 μl) of AP buffer supplemented with 3XFLAG peptide (Sigma) at 200 μg/ml at room temperature for 15 minutes on a Nutator.
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were dried and reconstituted in 0.1% formic acid and desalted by C18 ziptip (EMD Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of CHLI without transit peptide (1-46 amino acid residues) was cloned into pET32a (Novagen) and pGEX-4T-1 (GE healthcare ...
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bioRxiv - Biophysics 2022Quote: ... IP3R1 was eluted with 500 μM of T433 peptide and concentrated using a 100 kDa Amicon centricon (Millipore).
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and again with wash buffer before elution buffer (wash buffer with 300 ng 3x-FLAG peptide (Sigma Aldrich)) was added and incubated with beads overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Systems Biology 2021Quote: ... stringent wash were done with modified lysis buffer (250 mM NaCl + 20 µg/ml peptide FLAG (F3290 Sigma)) prior to elution with 200µg/ml peptide FLAG ...
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bioRxiv - Molecular Biology 2020Quote: ... Bound protein was eluted three times for 30 min by incubation with 0.3 mg/ml 3xFLAG peptide (Sigma) in BC100 at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted using Buffer A with 150 mM NaCl and 200 μg/ml 3XFLAG peptide (Sigma). Peak fractions were pooled ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Cell Biology 2021Quote: ... TiO2 beads (5010-21315, GL Sciences) were used to enrich phosphorylated peptide and homemade C8 (66882-U, Sigma) stageTip was used to trap the TiO2 beads for washes ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of each peptide was injected into the abdominal cavity of each mouse along with 0.25 mL Complete Freund’s Adjuvant (Sigma). To enhance the immune response ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP) before elution using wash buffer supplemented with 0.25 mg/mL 3xFLAG peptide (F4799, Sigma-Aldrich). The eluent was then subjected to another round of affinity purification using anti-HA resin (26181 ...
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bioRxiv - Genetics 2020Quote: ... log phase cells (OD600~0.5 - 0.6) with bar1Δ were arrested in late G1 phase using 50 ng/ml alpha-factor peptide (Sigma) for ~120 minutes ...
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bioRxiv - Biophysics 2021Quote: ... Target proteins were eluted with 5 column volumes of wash buffer plus 200 μg/mL FLAG peptide (Sigma). The eluent was then applied to Strep-Tactin Sepharose (IBA ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound peptides were eluted in three steps with a solution of 2 mg/ml Oxone (Potassium peroxymonosulfate, Sigma) in H2O ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were extracted using solutions of 50% and 80% acetonitrile (ACN) with 0.5% formic acid (Millipore), and the recovered solution dried down in a vacuum concentrator ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluates containing digested peptides were separated from beads by centrifugation through a 0.45 µM PVDF filter column (Millipore) and cysteines were subsequently reduced and alkylated by addition of 5 mM TCEP and 10 mM CAA for 30 min at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... GB1-TAZ2 binding to MITF peptides was determined utilising a polyhistidine monoclonal mouse antibody (1:5000, Sigma, H1029), incubated on the array for 4 hours at 4 °C ...