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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Recombinant Human X Linked Inhibitor of Apoptosis AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with HRP-linked secondary antibodies anti-mouse IgG (1:5000, A6782, Sigma-Aldrich, MO, USA) and anti-rabbit IgG (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA-cross-linked proteins were immunoprecipitated (1% kept as input) using 5 μg of NF-kB/p65 (Millipore) or control mouse IgG antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... and testosterone levels were measured using enzyme-linked immunosorbent assay kits (progesterone, Biovision, K7416-100; estrogen-17β, Sigma, ab108667 ...
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bioRxiv - Cancer Biology 2021Quote: ... and then reverse cross-linked with 1 μl of RNase A (10 μg/ml; Sigma-Aldrich; Cat#: R4642), followed by Proteinase K digestion (5 μl of 20 mg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were developed with appropriate secondary antibodies linked to HRP and visualized by chemiluminescence (Immobilon Western, EMD Millipore) using a ChemiDoc Imaging (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitation of the cross-linked Unkempt-RNA complexes was carried out using anti-Flag antibody (F1804, Sigma-Aldrich). Samples were processed exactly as described in (Buchbender et al. ...
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bioRxiv - Immunology 2019Quote: ... Then antibodies were cross-linked to the Sepharose by incubating with 20 mM dimethyl pimelimidate dihydrochloride (DMP, Sigma) in 0.1 M sodium tetraborate decahydrate (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 1mg of cross-linked chromatin from K562 cells was incubated with 10µg of anti-Flag antibody (Sigma, M2) pre-bound on 300 µl of Dynabeads Prot-G (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... Cross-linked chromatin was immunoprecipitated with either 5 μg anti-TCF4/TCF7L2 (Clone 6H5-3, #05-511, Millipore) or normal mouse control IgG (#12-371 ...
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bioRxiv - Cell Biology 2021Quote: ... The gelatin coating was then cross-linked using 0.5% glutaraldehyde and excess aldehyde quenched with sodium borohydride (Sigma) and washed with excess 1X PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The HRP reaction was initiated by addition of 3,3’,5,5’-tetramethylbenzidine enzyme-linked immunosorbent assay substrate solution (Sigma) and terminated after 30 min by adding H2SO4 (2M ...
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bioRxiv - Cancer Biology 2024Quote: ... GP5d and OE19 cells were cross-linked for 10 minutes at room temperature by using formaldehyde (Sigma, F8775). Sonicated chromatin was centrifuged ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... IL-1β and TNF-α using indirect sandwich enzyme-linked immunosorbent assay (ELISA) (Sigma-Aldrich; #RAB0308 and #RAB0477) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the levels of mouse HGF (Sigma-Aldrich, RAB0214), mouse EGF (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cross-linked for qPLEX-RIME 45 minutes after addition of 100 nM estradiol (E2) (Sigma-Aldrich) prepared in ethanol or ethanol (control).
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bioRxiv - Cell Biology 2024Quote: ... and then reverse cross-linked with 1 μl of RNase A (10 μg/ml; Sigma-Aldrich; Cat#: R4642), followed by Proteinase K digestion (5 μl of 20 mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL assay to label apoptotic and dead cells was performed using the ApopTag® Red In Situ Apoptosis Detection Kit (Millipore, cat. #S7165) following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Cancer Biology 2021Quote: After treatment with GSK343 +/− TRAIL for 24 hours apoptosis induction was assessed by Hoechst 33343 and propidium Iodide (PI) stain (Sigma-Aldrich, Dorset, England). Cells were incubated with 10μg/ml Hoechst 33342 and 10μg/ml PI at 37°C for 30 minutes examined using IX81 Olympus fluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Neuroscience 2021Quote: Lenti-X 293T (Takarbio 632180) cells were thawed in complete Lenti-X 293T media (DMEM (Millipore D5796) + 10% Tet-Free FBS (Takarabio 631101 ...
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bioRxiv - Cancer Biology 2020Quote: pLKO.1-puro lentiviral vectors expressing shRNAs targeting FASN (shFASN_1: NM_004104.x-1753s1c1 and shFASN_2: NM_004104.x-3120s1c1) were purchased from Sigma-Aldrich. An mCherry-LC3B lentiviral vector was kindly provided by Dr ...
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bioRxiv - Cell Biology 2021Quote: ... Lenti-X 293T cells at 75% confluency were transfected using X-tremeGENE 9 DNA transfection reagent (Sigma). The lentiviral plasmids used were pRSV-Rev (Addgene #12253) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... They were then washed and permeabilized 6 x 5mins in PBSTx (PBS plus 0.5% Triton-X (Sigma)) and blocked for 2 hrs room temperature in PBSTx plus 5% goat serum (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBST (0.1% Triton X-100 in 1X PBS; 3 x 5 minutes; Sigma-Aldrich, X100), and incubated with Click-iT Plus EdU reaction cocktail (Alexa Fluor 555 ...
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bioRxiv - Cancer Biology 2021Quote: Cells or tumor tissues were harvested and lysed in RIPA buffer complemented with cOmplete Mini Protease Inhibitor Cocktail inhibitors and Phosphatase inhibitor cocktails (Sigma). Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... two STAT-3 inhibitors (STAT-3 inhibitor III (WP-1066) and STAT-3 inhibitor XIII (C-188-9) were tested (Merck, Sigma) and WP-1066 was used for infection studies at a final concentration of 12 μM ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... sodium dodecyl phosphate (SDS) 0.1%, 25mM Tris) supplemented with protease and phosphates inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3) (Sigma-Aldrich) and containing 4% β-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... ubiquitin-conjugating enzyme E2 N (UBE2N) inhibitor, Ubc13 inhibitor (Rhyasen et al., 2013) and IκB Kinase inhibitor (PS-1145 dihydrochloride) were purchased from Sigma-Aldrich. LN cells from C57BL6 mice were treated with 10μM of IRAK-Inh ...
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bioRxiv - Cell Biology 2023Quote: ... that was supplemented with phosphatase inhibitors (2mM Sodium Vanadate,50mM Sodium Fluoride) and protease inhibitors (1mM PhenylMethylSulfonylFluoride (PMSF) and mammalian Protease Inhibitor mixture (Sigma)) directly to cells ...
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bioRxiv - Physiology 2024Quote: Cells were lysed using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (RIPA: Cat# R0278, PMSF: Cat# P7626, Protease Inhibitor Cocktail: Cat# P3840, and Phosphatase Inhibitor Cocktail 2: P5726, Sigma). Protein estimation was performed using DC protein assay (DC™ Protein Assay Kit II ...
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bioRxiv - Physiology 2024Quote: ... Protein lysates were prepared from rat RV tissue using modified RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (RIPA: Cat# R0278, PMSF: Cat# P7626, Protease Inhibitor Cocktail: Cat# P3840, and Phosphatase Inhibitor Cocktail 2: P5726, Sigma). Protein estimation was performed using DC protein assay (DC™ Protein Assay Kit II ...
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bioRxiv - Cell Biology 2020Quote: ... 1% glycerol) containing protease inhibitors (cOmplete Protease Inhibitor Cocktail (#11697498001, Sigma, Austria), yeast protease inhibitor cocktail (yPIC ...
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bioRxiv - Cell Biology 2020Quote: ... Inhibitors were obtained from Sigma Aldrich (Eg5 inhibitor STLC, 10 mM stock), Selleck (Plk1 inhibitor BI2536 ...
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bioRxiv - Developmental Biology 2021Quote: ... plus two inhibitors (KSOM+2i): 1 μM MEK inhibitor PDO325901 (Sigma-Aldrich) and 3 μM GSK-3 inhibitor CHIR9902 (Cayman Chemicals ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Cancer Biology 2020Quote: ... with a protease inhibitor cocktail and phosphatase inhibitor cocktail (both from Sigma). Protein concentrations were determined using a BCA protein assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40) that contains protease inhibitors and PhosSTOP inhibitors (Sigma-Aldrich). Cells were then centrifuged at 14,000 g for 10 min at 4°C to remove cell debris ...
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bioRxiv - Neuroscience 2022Quote: ... and 1/100 diluted phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2, Sigma-Aldrich). Samples were centrifuged at 20,000 x g for 20 minutes at +4°C and the supernatant was collected for further use ...
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bioRxiv - Molecular Biology 2022Quote: Inhibitors used in this study were Ezh2 inhibitor GSK343 (SML0766, Sigma-Aldrich), DNA methylation inhibitor 5-Azacytidine (A2385 ...
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bioRxiv - Biochemistry 2022Quote: ... protease inhibitor tablet (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Millipore Sigma), 1% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: ... containing phosphatase inhibitor cocktails 2 and 3 and protease inhibitors (Sigma-Aldrich), for 30–45min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... containing protease inhibitor cocktails and Halt phosphatase inhibitors (EMD Millipore, Billerica, MA). Protein quantification was performed using Coomassie Plus protein assay reagent (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% SDS) containing protease and phosphatase inhibitors (Protease Inhibitor Cocktail, Sigma-Aldrich, PhosSTOP Phosphatase Inhibitor Cocktail ...
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bioRxiv - Biophysics 2022Quote: ... protease inhibitors were added (cOmplete EDTA-free protease inhibitor cocktail, Sigma Aldrich) and the sample was dialyzed against NMR buffer (20 mM BisTris-HCl pH 6.5 ...
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bioRxiv - Cell Biology 2022Quote: ... in Mili-Q water) supplemented with protease inhibitors and phosphatase inhibitors (Sigma) for 45 min or 20 min at 4ºC ...