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Citations for Millipore Sigma :
1601 - 1650 of 3647 citations for Recombinant Human S100A11 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
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bioRxiv - Biochemistry 2023Quote: ... and the recombinant protein was eluted using buffer D supplemented with 10 mM reduced L-glutathione (Sigma–Aldrich). The purified protein was digested with TEV protease overnight ...
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Cell Biology 2023Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 250-500 μg of recombinant proteins were resuspended in HAT buffer supplemented with 0.75 mM acetyl CoA (Sigma) and 1 μl butyric acid (200 mM ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Bioengineering 2020Quote: ... of 0.3 with Edit-R lentiviral mKate2-tagged, constitutively expressed Cas9 nuclease under the hEF1α promoter (Dharmacon, CAS11229) in transduction medium containing 5 μg/mL polybrene (EMD Millipore, TR-1003-G). Complete growth medium was added to the transduced cells at a 3:1 dilution after 5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
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bioRxiv - Cell Biology 2020Quote: ... fibrinogen (human plasma derived) and WKYMVm were from Sigma Aldrich. Recombinant fNLFNYK ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with human rhinovirus 3C protease (8 units/ml; Novagen), and incubated without agitation (4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Human Dermal Fibroblasts were purchased from Sigma (cat # 106-05A). A vial of cells was thawed ...
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bioRxiv - Immunology 2021Quote: ... and human interferon-a 2B (15 ng/mL, Sigma-Aldrich). All cell counts were done manually using a hemocytometer and trypan blue exclusion.
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bioRxiv - Molecular Biology 2021Quote: ... human IPMK cDNA was subcloned into pGEX4T plasmid (Sigma Aldrich), expressed in Escherichia coli ...
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bioRxiv - Biochemistry 2020Quote: Human serum-derived Factor IX was purchased from Sigma Aldrich, MO ...
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bioRxiv - Microbiology 2022Quote: Human Cerebral Microvascular Endothelial cells hCMEC/D3 (obtained from Millipore) were grown in EndoGRO-MV complete media (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse anti-human nuclei clone 235-1 (1:250; Millipore), rabbit anti-histone H3.3K27M mutant (1:500 ...
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bioRxiv - Genomics 2020Quote: ... human collagen type III (88 ng per well; Sigma-Aldrich) or a combination of human collagen type I and type III (88 ng of each collagen type per well) ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v)
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bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Bioengineering 2019Quote: Human Umbilical Vein Endothelial Cells (UVECs) were obtained from Sigma® (200P-05N ...
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bioRxiv - Genetics 2019Quote: ... A rabbit anti-human FAH antibody (Cat. no. HPA041370; Sigma) was added in PBT at 1:200 and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4 °C.
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bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4°C prior to antibody staining.
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bioRxiv - Bioengineering 2019Quote: ... 1 U/ml human thrombin (EMD Millipore, Burlington, MA, USA) and 2 μg/ml ciprofloxacin (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20ng/ml human epidermal growth factor (hEGF) (Sigma, Cat. No.E9644) and allowed to proliferate over one or two passages ...
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bioRxiv - Immunology 2021Quote: ... were coated with either goat anti-human IgG (Sigma #I2136) or IgM (Sigma #I1636 ...
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bioRxiv - Molecular Biology 2020Quote: U2OS human cell lines were grown in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... 5 × 10−3 g/L human insulin (Sigma Aldrich, USA), 5 × 10−5 M hydrocortisone (Upjohn Laboratories SERB ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding shRNAs directed against human CDH13 mRNA (Sigma) were cloned into the pTRIPΔU3-EF1α-EGFP lentiviral vector ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-human β1 integrin monoclonal antibody (clone 12G10, Millipore).
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bioRxiv - Cancer Biology 2019Quote: ... Human lung carcinoma PC9 cells were obtained from Sigma-Aldrich, and H1299 cells were provided from ATCC (Manassas ...
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bioRxiv - Biochemistry 2020Quote: ... Goat anti-human IgG secondary antibody-Peroxidase (Fc-specific, Sigma) prepared at 1:3000 in PBST was then added and plates were incubated for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... Animals were ovulated using human chorionic gonadotropin (Sigma-Aldrich, C1063) according to Sive et al ...
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bioRxiv - Bioengineering 2020Quote: ... vascular endothelial growth factor 165 human (VEGF, H9166, Sigma Aldrich), Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 50 units/ mL human IL-2 (Sigma #11147528001).
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bioRxiv - Bioengineering 2020Quote: ... The human serum was purchased from Millipore Sigma (H4522-20ML). Human-cell-expressed SARS-CoV-2 Spike S1-His recombinant protein was provided by Sino Biological (40591-V08H) ...