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Citations for Millipore Sigma :
1601 - 1650 of 7146 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... all mice were provoked with a convulsive dose (60 mg/kg; i.p.) of the GABAa receptor antagonist pentylenetetrazole (PTZ; Sigma-Aldrich, Co.) to measure seizure susceptibility and evaluate seizure thresholds (Dhamne et al. ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the role of myosin II/RhoA in creating the refractory nature of the retracting losing front to receptor inputs (Fig. 5 and Supplementary Fig. 2) we pre-treated cells with either 50 μM nocodazole (Sigma Aldrich) or 10 μM Υ-27632 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Responsiveness to NGF was assayed by probing for the expression of tropomyosin receptor kinase A (Trk-A; 1:2000; #06-574, Millipore-Sigma) [48] ...
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bioRxiv - Neuroscience 2020Quote: ... Dimethyl sulfoxide (DMSO) and clozapine-N-oxide (CNO; agonist at hM3Dq receptors) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of CNO (0.1 mg/ml in saline solution containing 0.5% DMSO was prepared and stored in aliquots ...
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bioRxiv - Cell Biology 2021Quote: To explore the role of myosin II/RhoA and ERM proteins/cortical actin in creating a cell back refractory to receptor inputs we pre-treated cells with either 20 µM Υ-26732 (Sigma), or 50 µM Nocodazole (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... mice received vehicle or a cocktail of the a1-adrenergic receptor antagonist prazosin (0.5 mg/kg; Sigma-Adrich, St. Louis, MO) and the β-adrenergic receptor antagonist DL-propranolol (5 mg/kg ...
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bioRxiv - Neuroscience 2021Quote: ... with 10 pA increments in the presence of oxygenated R-aCSF supplemented with 10 μM gabazine and 1 μM strychnine (GABAA and glycine receptor antagonists, respectively; Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... For the blockade of cholinergic receptors in CA1 200nl of atropine (2 mM) and mecamylamine (2 mM) (1:1) (Sigma Aldrich) were microinjected at 1.2mm DV (IM-9B microinjector ...
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bioRxiv - Neuroscience 2022Quote: ... We blocked the nicotinic acetylcholine signaling pathway via application of the broad nicotinic receptor antagonists hexamethonium (Hex, 100 µM, Sigma Aldrich) and epibatidine (EPB ...
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bioRxiv - Neuroscience 2022Quote: Larvae were immobilized by immersion in a paralytic that specifically blocks nicotinic acetylcholine receptors present at the neuromuscular junction (20μL of 1 mg/mL α-bungarotoxin [Sigma Aldrich] in HEPES-buffered E3 [5 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Neuroscience 2023Quote: ... cells were treated for 1-2 hours prior to and for the duration of flow experiments with 1 µM S1P receptor antagonist fingolimod (FTY720) (stock 1 mM in DMSO, Sigma #SML0700). FTY720 experiments were performed with Protocol 1 in flow media supplemented with 0.3% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... mice and rats (male Sprague-Dawley, aged 7-10 weeks) were subcutaneously injected with the VEGF-receptor inhibitor SU5416 (Sigma-Aldrich) at a dosage of 20 mg/kg body weight under isoflurane anesthesia ...
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bioRxiv - Plant Biology 2021Quote: ... truncatula seedlings were transferred to Fahräeus medium supplemented with water (mock treatment) or 1 μM of 6-BAP (6-benzylaminopurine; Sigma-Aldrich) and maintained under the same growth conditions ...
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bioRxiv - Plant Biology 2019Quote: ... Inflorescences of each GUS line were pre-treated with ice cold acetone for 1h at −20°C and washed two times for 5 minutes with 100 mM sodium phosphate buffer followed by one wash with sodium phosphate buffer containing 1 mM K3Fe(CN)6 and 1 mM K4Fe(CN)6 (both Sigma) at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Genetics 2020Quote: ... Transduction was carried out by infecting 2x105 hES H1 cells per well of a 6-well plate with lentiviruses in the presence of 6 μg/ml Polybrene (Sigma) and 10 μM Y-27632 ROCK inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... Transduction was carried out by infecting 2×105 hES H1 cells per well of a 6-well plate with lentiviruses in the presence of 6 μg/ ml Polybrene (Sigma) and 10 μM Y-27632 ROCK inhibitor ...
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bioRxiv - Microbiology 2022Quote: ... Some of the groups were then treated with 100μM 6-aminonicotinamide (6-AN) or dehydroepiandrosterone (DHEA) (both from Sigma-Aldrich), two pentose phosphate pathway inhibitors18 ...
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bioRxiv - Neuroscience 2023Quote: ... Adult male mice of the NOD-SCID strain (∼30–35 g) were administered 2 µl of 6-hydroxydopamine (6-OHDA, Sigma) dissolved in PBS at a concentration of 3.75 µg/µl with the addition of 0.01% ascorbic acid at the rate of 0.5 µl/min during a stereotaxic surgery ...
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bioRxiv - Cell Biology 2023Quote: ... the newly formed bundles were kept in standard 2D muscle progenitor proliferation medium containing 1.5 mg/mL 6-aminocaproic acid (6-ACA) (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: C57BL/6 ES and C57BL/6-tdTomato+ ES cell lines were cultured on feeder cells with 2il medium: DMEM (Millipore) supplemented with 15% FBS (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Biophysics 2021Quote: ... 5(6)-Carboxyfluorescein was purchased from Sigma-Aldrich. Methyl-α-cyclodextrin (MαCD ...
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bioRxiv - Biophysics 2021Quote: ... D-Glucose 6-phosphate sodium salt (Sigma-Aldrich), D-(+)-Glucose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... 6-Diamidino-2-Phenylindole (DAPI) (Cat# 32670 Sigma) was used to stain the DNA ...
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bioRxiv - Cell Biology 2020Quote: ... acetylated tubulin (mouse, 6-11B-1, Sigma-Aldrich), ARL6 (rabbit ...
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bioRxiv - Cell Biology 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) was added at a final concentration of 20 ng/μl ...
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bioRxiv - Microbiology 2021Quote: ... 6 mM MnCl2 (Sigma-Aldrich, 7773-01-5), 0.7 mM dNTPs with 10 U SuperScript II reverse transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Glucose-6-phosphate dehydrogenase (G6PDH; Sigma ImmunoChemicals), anti-Rps24 (our laboratory) ...
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bioRxiv - Developmental Biology 2020Quote: ... 6-dodecanoyl-2-dimethylamino naphthalene (Laurdan; Sigma Aldrich). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Sigma, D9542-5MG) diluted to 1 mg/mL in water for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, D9542), as previously described[8] ...
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bioRxiv - Bioengineering 2022Quote: ... 0.84 v/v% chondroitin-6-sulfate (Sigma-Aldrich) and calcium nitrate tetrahydrate (Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: 6 kDa laminarin from laminaria digitata (Sigma, L9634) was dialyzed with a cut-off of 2 kDa to remove low molecular weight impurities ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.8per cent K3Fe(CN)6 (Sigma-Aldrich) for 1h at 4°C ...