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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Recombinant Human CD200 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Human Immunoglobulin G (IgG) (Millipore-Sigma) was fluorescently labeled with CFTM 568 maleimide dye (CF568 ...
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bioRxiv - Bioengineering 2024Quote: IgG from human serum (Sigma-Aldrich) was incubated at 62°C for 30 min to induce aggregation ...
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bioRxiv - Bioengineering 2024Quote: ... or human complement C1q (Sigma-Aldrich) were immobilized on a CM5 or C1 sensor chip (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... 4 mg/mL human fibrinogen (Sigma), 10% Matrigel (Corning) ...
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bioRxiv - Cancer Biology 2024Quote: ... raised against human ST3GAL1 (Sigma HPA040466) and ST3GAL2 (Abcam ab96028) ...
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bioRxiv - Microbiology 2024Quote: ... 10 % human AB serum (Sigma-Aldrich), and 10 ng/ml recombinant human macrophage colony stimulating factor (Peprotech ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% human serum albumin (Sigma-Aldrich), 0.0002% heparin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML). Medium was changed every three days until adult microglia were treated as indicated after 8 days in vitro (DIV).
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with FACS buffer (PBS, 2%FCS) and fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were cultured in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma), 50IU rhIL-2 (Peprotech ...
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bioRxiv - Molecular Biology 2022Quote: ... The media were supplemented with 10% FCS and 1% penicillin-streptomycin (Sigma-Aldrich, Taufkirchen, Germany). Cells were maintained at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... 200 μl MTT mix (DMEM supplemented with 10% FCS containing 250μg/ml tetrazolium bromide, Sigma) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML). Medium was changed every three days until adult microglia were treated as indicated after 8 days in vitro (DIV).
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... SMGs were digested in 500μl RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 6µl Collagenase-II (23mg/mL ...
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bioRxiv - Biophysics 2019Quote: ... with the addition of 10 mM HEPES and 0.5% fetal calf serum (FCS, Sigma-Aldrich) was used if not stated otherwise ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest) and 1% Penicillin–Streptomycin (PS, Sigma-Aldrich) at 37 °C and 5% CO2 in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... and re-suspended in ice cold 2 % (v/v) FCS/PBS (PAA Laboratories/Sigma Aldrich) following centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...
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bioRxiv - Immunology 2021Quote: ... 107 BAL cells per mL were suspended in 90% FCS and 10% DMSO (Sigma-Aldrich) and cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase) of mouse or rabbit origin (Sigma-Aldrich Inc.) were used ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA) and 120 µg/ml gentamicin (Refobacin ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 and BrM cells were cultured in 10% (v/v) FCS (Sigma-Aldrich)-supplemented RPMI-1640 (Sigma-Aldrich ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: ... The biotinylated extracts were incubated overnight at 4 °C with 100 μl of streptavidin-agarose suspension (S1638, Sigma). The next day ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of biotinylated S1-RBD to coated VNAR-hFc was detected by streptavidin-HRP (Merck Millipore, 18-152). The signal intensities were collected for each primary VNAR-hFc antibody without competitor and compared to the signal when a competitor antibody was present ...
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bioRxiv - Biochemistry 2019Quote: ... The target of interest is biotinylated either through sortagging or labeled with (+)-Biotin N-hydroxysuccinimide ester (Sigma-Aldrich). For NHS labeling ...
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bioRxiv - Neuroscience 2022Quote: ... Ctip2 or Brn2 incubated sections were reacted with appropriate biotinylated secondary antibody for 1 hour (B7139; Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: We synthesised biotinylated glutathione ethyl ester (bioGEE) by reacting sulfo-NHS-biotin (Thermo) with glutathione ethyl ester (Sigma) in 50mM NaHCO3 pH 8.5 for 2 hours at room temperature ...
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bioRxiv - Pathology 2023Quote: ... and then incubated with biotinylated secondary antibody goat anti-rabbit (1:400; Sigma-Aldrich Cat#B8895, RRID: AB_258649), in TBS-Sap for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated donkey antibody to rabbit IgG (10 μg/ml; AP182B, Merck Millipore) and then with Alexa488-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with biotinylated secondary antibody goat anti-rabbit (1:400; Sigma-Aldrich Cat#B8895, RRID: AB_258649) in TBS-Sap ...
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bioRxiv - Synthetic Biology 2023Quote: We mixed the biotinylated high-affinity (3F10) anti-HA antibodies (50□μg ml−1, ∼500□nM, Sigma-Aldrich) at a 2:1 ratio with Streptavidin (Sigma-Aldrich ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... washed with Binding Buffer and incubated with biotinylated anti-rabbit antibody in Binding Buffer (1:10,000, Sigma-Aldrich). After washing with Binding Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
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bioRxiv - Neuroscience 2021Quote: ... Peprotech) and recombinant rat IFNγ (reconstituted in pH 8 sodium phosphate [10mM; Sigma-Aldrich] were diluted to the required concentrations (LTα 1μg + IFNγ 75ng per injection ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of recombinant PRDX6 was induced with 1mM isopropyl β-d-thiogalactopyranoside (IPTG, Sigma). The culture was incubated for 20h at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... HMWHA or BCM− was pre-incubated with 300 IU/mL recombinant HYAL2 (Sigma-Aldrich) for 30 min prior to addition to co-cultures.
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bioRxiv - Molecular Biology 2020Quote: ... Digestion was performed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RNase R (30 pmols) or Bovine Serum Albumin (BSA, Sigma-Aldrich, MO, USA) were alone or combined with Csm5 WT or Csm5Δ46 (90 ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Cell Biology 2020Quote: Seeds of the relevant GFP-tagged lines were submerged in either glycerin or in solutions of NaCl (Sigma-Aldrich), mannitol (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...