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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Leptin Receptor LEPR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Plant Biology 2023Quote: ... Secondary antibody incubation was performed for 30min with 1:10000 (v/v) anti-rabbit IgG-peroxidase antibody (Sigma-Aldrich). Again ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse 1:10,000 or anti-rabbit 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... or NK92 cells with antibodies against acetylated (Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse, Sigma-Aldrich, Cat# T6793) and detyrosinated (Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker ...
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bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich, cat# ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used in this study: rabbit anti-EBF1 antibody (1:1000, AB10523; Millipore, Darmstadt, Germany,), mouse anti-MYO7A antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound primary antibodies were incubated with HRP-conjugated secondary antibodies (Amersham) and detected using chemiluminescence (Luminata HRP substrate, Millipore). List of antibodies used is listed in Supplementary Information 1.
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bioRxiv - Microbiology 2024Quote: ... Cells were then labelled with primary anti-RNAP-II antibodies (CTD Antibody, clone 8WG16, Sigma-Aldrich 05-952-I) and anti-VP (a gift from Anne-Sophie Gosselin Grenet ...
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bioRxiv - Molecular Biology 2023Quote: ... The beads were separated on a magnet and resuspended in 25 µl of cold Antibody buffer (Wash buffer with 0.1% BSA) containing respective primary antibody or IgG (TFE3, Sigma HPA023881 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in the primary antibody (anti-Kv3.2 antibody, 1:200, Cat.no. APC-011, alomone lab, Israel; anti-GAD67 antibody, 1:200, Cat.no. MAB5406, Sigma, USA) at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes probed with the appropriate primary antibodies were incubated with secondary antibodies linked to horseradish peroxidase (HRP) (Sigma). Blots were revealed using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... an anti-GFP polyclonal antibody (Medical & Biological Laboratories) and a rabbit horseradish-peroxidase-linked IgG whole antibody (Sigma-Aldrich) were used as the primary and secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... anti-FLAG antibody conjugated with horse radish peroxidase (A8592,1:10000) and HA antibody (SAB4300603,1:1000) were purchased from Sigma-Aldrich. Antibody against β-actin (sc-4777 ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Physiology 2023Quote: ... Mouse monoclonal anti-FLAG M2 antibody (F1804) and mouse monoclonal anti-β-actin antibody (A1978) were obtained from Sigma. HRP-conjugated goat anti-rabbit (CST#7074 ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies and antisera and dilutions were used: mouse anti-tubulin antibody (1:250, Millipore Sigma, cat# T5168); mouse anti-CSP (clone 2A10 ...
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bioRxiv - Microbiology 2020Quote: ... the samples were probed with rabbit polyclonal anti-Spo0A or anti-FliC antibodies at a 1:1000 dilution and an anti-rabbit secondary antibody conjugated to horseradish peroxidase (Sigma) was used at dilution 1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... in primary antibodies for 24 hours at 4°C [Primary antibodies: Mouse anti-tyrosine hydroxylase (TH) clone LNC1 (1:500; Millipore) and rabbit anti-vesicular monoamine transporter 2 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes were subsequently incubated overnight at 4 °C with primary antibodies (1:2,000 monoclonal mouse anti-TRPA1 antibody, Sigma-Aldrich, and 1:3,000 MF20 mouse anti-myosin heavy chain antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... Myofibers present in a muscle section were stained with an anti-myosin antibody (MF 20, DSHB) while anti-laminin antibody (Sigma) was used to visualize the outline of all myofibers present in a given muscle section ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were loaded and run on SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted with antiKv2.1 antibody (K89/34, NeuroMab) and anti-α tubulin antibody (Sigma). The blots were detected using chemiluminescence reagent on a Bio-Rad Chemidoc MP and Image J was used for quantification of detected bands.
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bioRxiv - Molecular Biology 2020Quote: ... antibodies, and the secondary antibodies used included anti-rabbit (31460, Pierce), anti-mouse (31430, Pierce) and anti-rat (A9037, Sigma) HPR antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... AtMPK4 and AtMPK6 was performed using anti-MAP kinase-antibody (1:20000 dilutions) as primary antibody and anti-mouse IgG (Sigma) protein (1:15000 dilutions ...
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bioRxiv - Biochemistry 2019Quote: ... anti-actin (A4700) and anti-tubulin antibody (T6199) and goat anti-human IgG–HRP antibodies were obtained from Sigma-Aldrich, Dorset ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were stained with primary antibodies diluted 1:1,000 in TTBS followed by incubation with horse peroxidase-labeled secondary antibody (Millipore Sigma) diluted 1:10,000 in 2% non-fat milk in TTBS ...
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bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat. # G4595) and mouse monoclonal antibody against GAPDH (Cat. # G8795) were purchased from Sigma Aldrich. Rabbit polyclonal antibody against Peroxiredoxin-SO3 (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-α-tubulin primary antibody as well as secondary antibodies coupled to horseradish peroxidase (HRP) were obtained from Sigma-Aldrich. AlexaFluor-labelled secondary antibodies were obtained from ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation reactions were performed using anti-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, F1804, Sigma-Aldrich). The complex of chromatin antibody was captured with protein G beads (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot of phosphorylated MAPKs was performed using anti-phospho p44/42 MAPK antibody as the primary antibody and peroxidase-conjugated goat anti-rabbit IgG (Sigma) as the secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2021Quote: ... that utilizes the same human antibodies.12 Antibodies were prepared 1:500 in carbonate-bicarbonate coating buffer (SRE0034, Sigma-Aldrich), added to each well of a Costar 96-well Assay plate (3369 ...
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bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were immunodetected using 1:2500 dilution of the rabbit anti-TbKH polyclonal antibody and 1:15,000 dilution of goat anti-rabbit-HRP antibody (Sigma-Aldrich). The chemiluminescent protocol was used for developing as indicated below.
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal mouse anti-Flag (M2) and anti-MAP2 (HM-2) antibodies as well as polyclonal rabbit anti-Flag antibodies (F3165, F7425) were from Sigma. Anti-Xpress antibodies were from Invitrogen (46-0528).
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bioRxiv - Neuroscience 2022Quote: ... blocked in PBST and 10% NGS for 2–3 hours at room temperature, and incubated in rat anti-mCherry antibody (1:2000, Life Sciences) and rabbit anti-TH antibody (1:1000, Millipore) at 4°C for four nights ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with an alkaline phosphatase-conjugated secondary antibody directed against the first antibody (Sigma Aldrich, Taufkirchen, Germany) for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse monoclonal antibodies against MAP2 (M4403, IC: 1:100) and mouse monoclonal antibodies against GAPDH (WB: 1:1000) were from Sigma. Mouse monoclonal antibodies against GAD65 (GAD-6 supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-β-actin antibody (catalog #: A1978) and mouse monoclonal FLAG antibody (catalog #: F1804) came from Sigma Aldrich. The fluorescent anti-β-actin antibody Rhodamine came from Biorad (catalog # ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated with primary antibody overnight at 4 °C [1:500 goat anti-RNA polymerase II antibody (PLA0292, Sigma) with 1:500 rabbit anti-PCNA antibody (PLA0079 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in 1:50 dilution of biotinylated anti-lucifer yellow antibody (RRID: AB_2536191) in universal antibody dilution solution (Sigma Aldrich) for 3 days at 4°C with occasional gentle agitation ...
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bioRxiv - Microbiology 2020Quote: ... In this case the blot was incubated with the IcdH primary antibody at room temperature for 2 hours and with the secondary antibody (anti-rabbit HRP, Sigma) was done at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... or incubations with species specific biotinylated antibodies (Vector, 1:100), then streptavidin-HRP antibody (Vector, 1:200) before development with diaminobenzidine (Sigma). Counterstaining was performed using toluidine blue ...