Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 4285 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... cloned into a pET-47b bacterial expression plasmid (Novagen) between SmaI and NotI restriction sites in frame with an N-terminal 6x His-tag and an HRV 3C Protease cleavage site for fusion tag removal ...
-
bioRxiv - Bioengineering 2021Quote: ... and ligated into the backbone of eSpCas9_PuroR_GFP plasmid (Sigma) using BbsI cut sites and T7 ligase (NEB) ...
-
bioRxiv - Neuroscience 2021Quote: ... The PCR product was ligated into pET28a(+) plasmid (Novagen), which was linearized with Nhe I and BamH I ...
-
bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Yeast genomic DNA was isolated with the SDS/LiAc protocol [29] ...
-
bioRxiv - Cancer Biology 2020Quote: Plasmids for TS knock down (TRCN0000456666) is from Sigma. Empty backbone pLKO.1 (referred to as pLKO ...
-
bioRxiv - Biophysics 2020Quote: ... inserted into a pET-30 plasmid (Novagen, Madison, WI) were transformed into BL21 (DE ...
-
bioRxiv - Biophysics 2021Quote: ... Plasmids were transiently transfected using Genejuice (Novagen/EMD Millipore) into HEK293T cells ...
-
bioRxiv - Biochemistry 2022Quote: ... expanded and the plasmid DNA was extracted (Sigma, PNL350). Lentiviruses were generated in HEK293T cells and then used to infect Cas9 cells ...
-
bioRxiv - Biophysics 2022Quote: ... The plasmids were transformed into BL21(DE3) cells (Sigma) using heat-shock ...
-
bioRxiv - Cancer Biology 2022Quote: ... the MISSION TRC2 pLKO.5-puro plasmid (Sigma-Aldrich) was modified to replace the puromycin-resistance gene with the enhanced green fluorescent protein (EGFP ...
-
bioRxiv - Cell Biology 2020Quote: ... The 3xFlag sequence from p3xFLAG-CMV-7.1 plasmid (Sigma) was inserted downstream of the AID sequence ...
-
bioRxiv - Cancer Biology 2019Quote: ... Control and knockdown ADAMDEC1 plasmids were purchased from Sigma. Different clones for each shRNA plasmid were tested and the best knockdowns were selected to produce lentiviral particles ...
-
bioRxiv - Bioengineering 2019Quote: ... coli expression and cloned into a pET28a(+) plasmid (Novagen) with an N-terminal hexahistidine-tag and C-terminal GSGRRRRRRRR sequence ...
-
bioRxiv - Microbiology 2020Quote: ... coli using a Sigma GenElute Plasmid Kit (Sigma Aldrich). Plasmids used in this study are shown in Table 4 ...
-
bioRxiv - Cell Biology 2019Quote: ... The 3xFlag sequence from p3xFLAG-CMV-7.1 plasmid (Sigma) was inserted downstream of the AID sequence ...
-
bioRxiv - Biophysics 2021Quote: ... Plasmids were transiently transfected using Genejuice (Novagen/EMD Millipore) into HEK293T cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmid p3XFLAG -CMV-7.1 was obtained from Sigma (E7533).
-
bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
-
bioRxiv - Neuroscience 2022Quote: ... and the plasmids mixed with Fast Green dye (Sigma) were microinjected into one of the lateral ventricles for CPN circuit analysis and axon GC isolation experiments ...
-
bioRxiv - Biophysics 2023Quote: ... UBQLN2-ΔUBA and ubiquitin (Ub) in pET24b (+) plasmid (Novagen) were grown in Luria-Bertani (LB ...
-
bioRxiv - Immunology 2023Quote: ... CAS9 Blasticidin Lenti plasmid was purchased from Sigma (CAS9BST). Plasmids were expanded in house and isolated using Plasmid Plus Midi Kit (Qiagen ...
-
bioRxiv - Plant Biology 2023Quote: ... Plasmids were transformed into E.coli Rosetta2 (DE3) cells (Novagen). Bacteria were grown in LB medium at 37 °C up to an OD600nm of 0.6 ...
-
bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA constructs were cloned into pET-24b (Novagen) expression vector by site-directed mutagenesis and the coding sequences of all mutants were confirmed by DNA sequencing prior to use.
-
bioRxiv - Cancer Biology 2022Quote: ... Plasmids were separately transfected into BL21-DE3-Rosetta (Novagen). Proteins were expressed overnight at 18°C in TB medium (Melford ...
-
bioRxiv - Genomics 2023Quote: ... The plasmid library was prepared by maxi-prep (Sigma), digested with SphI-HF and SpeI-HF (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... when needed for plasmid maintenance or selection (Sigma-Aldrich). Escherichia coli was grown at 37°C in LB medium with 100 μg/ml ampicillin and/or 20 μg/ml chloramphenicol (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2023Quote: ... into the pTriEx™-1.1 Hygro plasmid (Novagen 70928). Stably expressing cell lines were generated using FuGENE® HD Transfection Reagent (Promega) ...
-
bioRxiv - Biochemistry 2023Quote: ... and subcloned into a modified pCDF-1b plasmid (Novagen) resulting in expression of MsbA with an N-terminal TEV protease cleavable His6 fusion protein ...
-
Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
-
bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
-
bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
-
bioRxiv - Cell Biology 2020Quote: ... fibrinogen (human plasma derived) and WKYMVm were from Sigma Aldrich. Recombinant fNLFNYK ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with human rhinovirus 3C protease (8 units/ml; Novagen), and incubated without agitation (4°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Human Dermal Fibroblasts were purchased from Sigma (cat # 106-05A). A vial of cells was thawed ...
-
bioRxiv - Immunology 2021Quote: ... and human interferon-a 2B (15 ng/mL, Sigma-Aldrich). All cell counts were done manually using a hemocytometer and trypan blue exclusion.
-
bioRxiv - Biochemistry 2020Quote: Human serum-derived Factor IX was purchased from Sigma Aldrich, MO ...
-
bioRxiv - Microbiology 2022Quote: Human Cerebral Microvascular Endothelial cells hCMEC/D3 (obtained from Millipore) were grown in EndoGRO-MV complete media (Millipore ...
-
bioRxiv - Neuroscience 2021Quote: ... Mouse anti-human nuclei clone 235-1 (1:250; Millipore), rabbit anti-histone H3.3K27M mutant (1:500 ...
-
bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
-
bioRxiv - Genomics 2020Quote: ... human collagen type III (88 ng per well; Sigma-Aldrich) or a combination of human collagen type I and type III (88 ng of each collagen type per well) ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v)
-
bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium ...
-
bioRxiv - Bioengineering 2019Quote: Human Umbilical Vein Endothelial Cells (UVECs) were obtained from Sigma® (200P-05N ...
-
bioRxiv - Genetics 2019Quote: ... A rabbit anti-human FAH antibody (Cat. no. HPA041370; Sigma) was added in PBT at 1:200 and incubated overnight at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
-
bioRxiv - Genomics 2020Quote: ... and/or 0.4μg human GST-tagged PP2A-α (Sigma, SRP5336) in the presence or absence of AZ’5576 or okadaic acid (Abcam ab120375 ...
-
bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4 °C.