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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Goat Anti Mouse IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The negative control group was incubated with normal mouse IgG (Millipore). Samples were incubated with proteinase K buffer to isolate immunoprecipitated RNA ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... Negative controls included a mouse IgG (2µg/mL, Sigma, for Col2) or rabbit IgG (1µg/mL ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitations were performed using mouse αFlag IgG (5µg; F1804, Sigma-Aldrich), mouse αGFP IgG (4 µg ...
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bioRxiv - Immunology 2023Quote: ... Control mice received 100µg of IgG from mouse serum (Sigma, I5381) by i.p ...
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bioRxiv - Genetics 2024Quote: ... three DRIPs were performed: normal mouse IgG (Millipore-Sigma # 12-371), S9.6 antibody without RNAse H treatment and RNAse H treatment followed by S9.6 antibody ...
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bioRxiv - Genetics 2024Quote: ... three DRIPs were performed: normal mouse IgG (Millipore-Sigma # 12-371), S9.6 antibody without RNAse H treatment and RNAse H treatment followed by S9.6 antibody ...
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bioRxiv - Immunology 2023Quote: ... α-tubulin and mouse IgG MOPC were from Sigma Aldrich (USA). Mouse α-WDR1 was from Santa Cruz (USA) ...
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bioRxiv - Plant Biology 2021Quote: ... 1:1000 for goat-α-mouse-HRP (AP124P, EMD Millipore), and 1:10000 for goat-α-rabbit-HRP (AS09 602 ...
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bioRxiv - Cell Biology 2020Quote: ... and goat α-mouse–HRP (Sigma–Aldrich; RRID:AB_258476, 1:50,000). All blocking and incubations were done in 5% (wt/vol ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... secondary peroxidase conjugated goat a-rabbit or mouse antibodies (Sigma) were used ...
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bioRxiv - Microbiology 2020Quote: ... secondary peroxidase conjugated goat α-rabbit or mouse antibodies (Sigma) were used.
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bioRxiv - Genetics 2019Quote: ... 10% FCS (Sigma) supplemented with FLT3L (50 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 % FCS (Sigma) and P/S (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... + 10% FCS (Sigma,) + 1 x (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... 10% FCS (Sigma), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FCS (Sigma), NEAA ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % FCS (Sigma) in DPBS for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatant was incubated overnight with normal goat serum-adsorbed IgG beads (Sigma) and then with α-HA-coupled beads (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... drained and incubated for 15 min in 0.1% (w/v) Goat IgG (Sigma Aldrich) (in PBS/BSA ...
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bioRxiv - Synthetic Biology 2023Quote: ... The primary and secondary antibodies were 1:2000 rabbit α-GAPDH (Sigma-Aldrich)/3% BSA/TBSA and 1:20,000 HRP-conjugated goat α-rabbit IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Neuroscience 2021Quote: ... 1x phosphatase inhibitor II,1x phosphatase inhibitor III (Sigma), before centrifugation of cell lysates at 20000 g for 30 min at 4 □ ...
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bioRxiv - Molecular Biology 2021Quote: Phosphatase activity was measured using phosphatase assay kit (Millipore). Immunoprecipitated samples were resuspended in phosphate-free assay buffer ...
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bioRxiv - Immunology 2021Quote: ... phosphatase inhibitors (Phosphatase Inhibitor Cocktail 1 and 2, Sigma), 3mM PMSF and 2mM pervanadate ...
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bioRxiv - Genomics 2021Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor 1 and 2, Sigma), followed by sonication (Branson Sonifier ...
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bioRxiv - Cell Biology 2021Quote: ... and phosphatase inhibitors (Sigma phosphatase inhibitor 2 and 3), then sonicated using a Fisher sonicator at 12% amplitude for total of 20 seconds ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich). One volume of 2× Laemmli buffer (100 mM tris-HCl [pH 6.8] ...
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bioRxiv - Immunology 2020Quote: ... and phosphatase inhibitors (phosphatase inhibitor cocktail II, Sigma, #P5726). 2 mM of TCEP (#C4706 ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich). One volume of 2X Laemmli buffer (100 mM Tris-HCl [pH 6.8] ...
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bioRxiv - Neuroscience 2021Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3, Sigma-Aldrich) followed by homogenization using QIAshredder spin columns (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... and phosphatase inhibitors (Phosphatase Inhibitor Cocktail Set II, Millipore). Cells were lysed with glass beads in a cooled FastPrep (MP Biomedicals) ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used are polyclonal goat anti-TrkB (AF1494, Bio-Techne Sales Corp; 1:500) and monoclonal mouse anti-α-tubulin (T5168, Sigma-Aldrich; 1:5000). The secondary antibodies used are peroxidase AffiniPure donkey anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: ... Alkaline stress medium consists of RPMI 1640 (Sigma), supplemented with 1% IFS and 10 μg/mL gentamicin ...
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bioRxiv - Microbiology 2024Quote: ... For color development 50 µl alkaline hypochlorite (Sigma) were added and absorption was measured after 1 h (Tecan plate reader ...
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bioRxiv - Neuroscience 2019Quote: ... Cleared supernatants (500 μg) were mixed with 2 μg IgG control (Sigma Aldrich, 12-371 (normal mouse IgG control) or 12-370 (normal rabbit IgG control) ...
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bioRxiv - Cancer Biology 2020Quote: ... serum samples used to detect levels of IgG by anti-IgG-HRP antibody (Bio-Techne) or IgM by anti-IgM-HRP antibody (Sigma) on 100μg/ml NP-KLH-coated 57 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated with anti-mouse NeuN or anti-mouse parvalbumin (Millipore, MAB377 ...
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bioRxiv - Microbiology 2021Quote: ... a mouse anti-FLAG primary (Sigma Mouse Anti-FLAG M2 monoclonal antibody) was used ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-pERK1/2 (T185/Y187) and mouse anti-Flag M2 (Sigma); and mouse anti-H3 and rabbit anti-H3K4 trimethyl (Active Motif).
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... NF-kB (CST, #3033), PCM1 (CST, #5213) and GABARAP (CST, #26632) and NDP52 (. Secondary antibodies: anti-Mouse IgG–Peroxidase (Sigma, A5906), anti-Rabbit IgG–Peroxidase (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... cell lysates were subjected to immunoprecipitation overnight using an anti-mouse HSF2 (Santa-Cruz) and a non relevant IgG (Sigma-aldrich) as a negative control that were pre-incubated 1h at RT with Protein G UltraLink Resin beads (53132 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... the blots were incubated at room temperature on a rocker for 1 hr with secondary anti-mouse IgG-HRP (Sigma, A4416) or anti-rat IgG-HRP (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with anti-mouse IgG peroxidase-conjugated (1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) at RT for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... the membrane was incubated for 1h at room temperature with the secondary antibody anti-mouse IgG peroxidase-conjugated (1/500; A5906, Sigma-Aldrich). The revelation was performed using Pierce™ ECL Plus Western Blotting Substrate (32312 ...
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bioRxiv - Biochemistry 2020Quote: ... Ribosomal protein L3 was detected using anti-L3 primary antibodies (a gift from Fujio Kawamura; 1:20,000 dilution) combined with goat anti-rabbit IgG-HRP secondary antibodies (Sigma-Aldrich, A0545; 1:10,000 dilution). ECL detection was performed using WesternBright™ Quantum (K-12042-D10 ...