Labshake search
Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Cow Fructose 1 6 Bisphosphatase 2 FBP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... the cells were treated with 6-hydroxydopamine hydrochloride (6-OHDA, 15 µM, H4381, Sigma Aldrich) and METTL3/14 activators (100 nM or 1 µM) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...
-
bioRxiv - Biophysics 2020Quote: ... 2 mM CaCl2 and 10 mM HEPES at pH 7.4 with 1-2 mM qBBr (Sigma-Aldrich or Toronto Research Chemicals ...
-
bioRxiv - Microbiology 2022Quote: ... and 2 µg/ml L-1-Tosylamide-2-phenylethyl-chloromethyl-ketone (TPCK)-treated trypsin (Sigma-Aldrich, USA). Viral infectivity titers were measured in MDCK-II cells in 96-well plates ...
-
bioRxiv - Microbiology 2019Quote: ... and alternative NADH dehydrogenase inhibitor flavone (2-Phenyl-4H-1-benzopyran-4-one, 2-Phenylchromone, Sigma-Aldrich) (Martins et al ...
-
bioRxiv - Neuroscience 2020Quote: ... protease inhibitor cocktail tablets and 2 mg/mL pepstatin A dissolved in ethanol:dimethyl sulfoxide 2:1 (Sigma). To minimize inter-blot variability ...
-
bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
-
bioRxiv - Microbiology 2023Quote: ... Fur was submerged in 2 mL of 2:1 (v/v) chloroform:methanol (Sigma 288306 and Sigma 322415) followed by sonication in a water bath for six minutes to dislodge lipids ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell pellets were resuspended in 200µl of LCMS-grade acetonitrile/methanol/water (2:2:1) (Sigma Aldrich) chilled to -20°C and transferred to lysing matrix Y tubes (MP Biomedicals ...
-
bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
-
bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
-
bioRxiv - Genetics 2023Quote: Tissue lysates in RIPA buffer containing protease and phosphatase inhibitors as above were used for ELISA measurements of IL-1 beta (#RAB0275, Sigma Aldrich, GmbH) and IL-6 (#RAB0309 ...
-
bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.02% ascorbic acid was added to a sterile saline solution (NaCl, 0.9% w/v) and 6-OHDA HCl powder (6-hydroxydopamine hydrochloride from Sigma or 6-OHDA hydrobromide from Tocris) was then dissolved to produce a final 6-OHDA concentration of 4.44 mg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... This was after 2 washes in Buffer A (Sigma DUOlink PLA kit) and then incubated with the PLA kit Ligation solution (1X ligation buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-alpha-tubulin (Sigma, Cat no. T5618, Clone B-5-1-2; 1:200), anti-Muc1 (Cell Signaling Technologies ...
-
Fussing about fission: defining variety among mainstream and exotic apicomplexan cell division modesbioRxiv - Microbiology 2020Quote: ... mouse ascites MAb B-5-1-2 α-tubulin 1:200 (Sigma-Aldrich #T5168); rat TgEB1 1:100 (Chen and Gubbels ...
-
bioRxiv - Neuroscience 2019Quote: Complete Freund’s Adjuvant (CFA, 20 µl, 2% in 1:1 saline) (Sigma-Aldrich, F5881) was injected into the hindpaw of mice ...
-
bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
-
bioRxiv - Microbiology 2020Quote: ... and mouse anti-α-tubulin 1:10,000 (monoclonal B-5-1-2, Sigma-Aldrich). To image the blot the secondary antibodies goat anti-rabbit IgG IRDye 800CW (LI-COR ...
-
bioRxiv - Developmental Biology 2022Quote: ... and diphospho-ERK-1 and ERK-2 (1:4000; clone MAPK-YT; Sigma; RRID:AB_477245). In our hands ...
-
bioRxiv - Cell Biology 2020Quote: ... Sections were picked up in a 1:1 mixture of 2% methylcellulose (Sigma-Aldrich) and 2.3 M sucrose ...
-
bioRxiv - Physiology 2019Quote: ... 1% penicillin-streptomycin and 2 μg.ml−1 puromycin dihydrochloride (Sigma, St. Louis, MO, USA) were added the next day ...
-
bioRxiv - Plant Biology 2021Quote: ... 2 g L-1 Phytagel and 1.5 g L-1 activated charcoal (Sigma-Aldrich). After inoculation ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). For tissue samples ...
-
bioRxiv - Neuroscience 2022Quote: ... the supernatant was mixed 1:1 with RIPA buffer with 2% sarkosyl (Sigma L9150) and incubated for 1 hour at room temperature with shaking ...
-
bioRxiv - Microbiology 2022Quote: ... and then 2 ml overlay comprising 1:1 viral growth medium: 2.4% cellulose (Sigma) was added ...
-
bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were: α-Tubulin (Sigma, B-5-1-2; T5168, 1:100,000), MCM2 (Abcam ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were overlaid with 1:1 volume of 2 % methyl cellulose (Sigma, USA) in 2X MEM supplemented with 5 % FBS and plate was incubated for 48 h ...
-
DTX3L and USP28 fine-tune DNA double strand repair through mutual regulation of their protein levelsbioRxiv - Biochemistry 2023Quote: ... and against α-tubulin (clone B-5-1-2) (#T5168; 1:10.000; Sigma-Aldrich). Polyclonal antibodies in this study were used against USP28 (#HPA006778 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 mM MgCl2) with 1% SDS and 1:2,000 Benzonase® nuclease (Sigma E1014). Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek) ...
-
bioRxiv - Cell Biology 2019Quote: Cold KIF3A purification buffer (50 mM NaPi, 400 mM KCl, 2 mM MgCl2 × 6 H2O, 0.001% Brij35, 0.75 mM β-mercaptoethanol, Sigma#M3148, 0.2 mM ATP) supplemented with protease inhibitors and DNaseI was added to frozen BL21-RIL cell pellets expressing a KIF3A construct ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
-
bioRxiv - Neuroscience 2021Quote: ... per well of a 6 well plate of differentiated human neural cultures (~2 months old) along with 3μg/ml of polybrene (Sigma-Aldrich #TR-1003-G), pipetting the LV concentrate directly onto the culture (drop-wise) ...
-
bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... spun down again and resuspended the cells in 200 µL of 1X PBS (phosphate buffered saline) with 1.5 μl 4′,6- diamidino-2-phenylindole (DAPI, Sigma-Aldrich, 100 ng/ml). DAPI stains dead cells ...
-
bioRxiv - Biophysics 2023Quote: ... The mass of the oligonucleotides was determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS, Bruker, AutoFlex Max) using 2’,6’-dihydroxyacetophenone (DHAP, Sigma, Cat. No. 37468) as a matrix ...
-
bioRxiv - Biochemistry 2020Quote: ... fixed and stained for β-galactosidase activity at pH = 6 using Senescence Cells Histochemical Staining Kit (Sigma, Cat#CS0030). For rescue assays ...
-
bioRxiv - Developmental Biology 2021Quote: ... were obtained from the Developmental Studies Hybridoma Bank (DSHB), anti-acetylated microtubule antibody (6-11, b-1) was obtained from Sigma (1:200), anti-Lky (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... to block non-specific staining and incubated with a mouse anti-acetylated tubulin antibody (Clone 6-11b-1, 1:100; Sigma Cat# T6793) for 4 h at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... 0.003% 1-phenyl-2- thiourea (Sigma, St Louis, MO, P7629) was added to EM from 20-24 h postfertilization to prevent pigment formation ...
-
bioRxiv - Biophysics 2020Quote: ... and 1 mM Tris(2-carboxyethyl)phosphine (Sigma Aldrich, C4706). 1 mL of 10X EDTA free protease inhibitor cocktail (SIMGA FAST protease inhibitor tablets ...
-
bioRxiv - Cell Biology 2022Quote: ... 2-diphenyl-1-picrylhydrazyl (DPPH) were procured from Sigma-Aldrich. Sodium chloride ...
-
bioRxiv - Neuroscience 2022Quote: ... propyl acetate and 3-methyl-2-buten-1-ol (Sigma). Odorants were selected based on previous work using this task (Gu & Li ...
-
bioRxiv - Molecular Biology 2021Quote: Anti-α-tubulin (Sigma-Aldrich, clone B-5-1-2), anti-METTL18 (PROTEINTECH GROUP ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1 μM 5-Fluoro-2’-deoxyuridine (FUdR, Sigma-Aldrich) for 3.5 hours at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1 µM PD0325901/MEK1/2 Inhibitor (Millipore Sigma 444968) in DMEM ...
-
bioRxiv - Microbiology 2019Quote: ... 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES; Sigma Aldrich), Sodium chloride (NaCl ...
-
bioRxiv - Immunology 2020Quote: ... or 1 μM MMP-2/MMP-9 inhibitor I (Millipore). Cytotoxicity assays were then performed as described above.
-
bioRxiv - Cancer Biology 2019Quote: ... inhibitor of dynamin 1 and 2 (80 mM; Sigma-Aldrich); Hoechst 33342 nuclear stain (20 mM ...