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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 7 Bromo 2 methylimidazo 4 5 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Cell Biology 2020Quote: ... Thin-layer chromatography (solvent system chloroform : methanol : 4.2N ammonium hydroxide 9:7:2) was done as described (111) on aluminium silica TLC plates (Sigma) for 75 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Cell Biology 2021Quote: Cellular ROS was measured by incubating cells with 100 µM 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) (D6883, Sigma-Aldrich) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cancer Biology 2020Quote: Intracellular ROS levels were measured by monitoring the oxidation of cell permeable 2′,7′-dichlorofluorescein diacetate (DCFHDA, Sigma-Aldrich) to fluoros-pectrophotomete at excitation and emission wavelengths of 480 and 535 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Three-mL liquid cultures were inoculated with a single colony and supplemented with 2′,7′-dichlorodihydrofluorescein diacetate (DCFHDA; Sigma) with a final concentration of 1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Immunology 2023Quote: ROS production in BMDMs was measured using the membrane-permeable fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFHDA) (Sigma, #D6883). BMDMs were plated in 96-well plates at 1×105 cells/well ...
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bioRxiv - Genetics 2023Quote: ... 30 hours post-fertilization old embryos were exposed to 20 μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-Aldrich) for 1 hour at 28.5 °C in the dark followed by washing with embryo water a minimum of three times 31 ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... CYP1A2 and CYP2A6 activities were measured by incubating cultures with 5 μM 7-ethoxyresorufin or 50 μM coumarin (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Biophysics 2021Quote: COS-7 cells transfected with WT tau alone or with fyn kinase were similarly stained with tau-5 (Sigma-Aldrich) or with tau-9g3 primary antibody (GeneTex Inc. ...
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bioRxiv - Microbiology 2019Quote: ... The extract was spotted onto the TLC plate as follows: 7 μl of bacterial extract or 5 μl of 20 μM PQS synthetic standard (Sigma). PQS quantity was then determined by exposing resolved TLC plates to 325 nm UV light ...
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bioRxiv - Cell Biology 2021Quote: ... Mx-cre+ x Metflox/− offspring (5-7 wk) received five injections of 300 μg polyinosinic/polycytidylic acid (pI:pC, Sigma-Aldrich) in PBS i.p ...
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bioRxiv - Neuroscience 2022Quote: ... mice were given ad libitum access to 5 mg of clozapine-N-oxide (CNO; Sigma-Aldrich cat. #34233-69-7) dissolved in 200 mL of drinking water.
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bioRxiv - Systems Biology 2019Quote: ... 0.01 g/l CaCl2 ×2H2O) were supplemented with 5 g/l Peptone N-Z-Soy BL 7 (Sigma-Aldrich S.r.l) (complex medium ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were differentiated into iPSC-CMs using the Wnt modulation-differentiation method (Lian et al., 2012) with 7 μM CHIR (Selleckchem) and 5 μM IWP2 (Sigma) in RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... After selection for plasmid integration with 1 μg/ml puromycin for 5-7 days, cells were induced for effector (CasRx, DjCas13d or other Cas13d orthologs) expression using Doxycycline (D3072, Sigma). 3-5 days after induction ...
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bioRxiv - Cell Biology 2020Quote: ... and was centrifuged twice at 300 g for 10 min at 4 °C in a Sigma 4-16KS centrifuge (Sigma Laboratory Centrifuges) to remove cells and the supernatant was stored at −80 °C until use.
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bioRxiv - Neuroscience 2022Quote: ... and the cochleae were fixed in 4% paraformaldehyde (PFA) overnight at 4 °C and then decalcified in 10% EDTA (#798681, Sigma, Darmstadt, Germany) in PBS for 2–3 days at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... Specimens for histamine immunohistochemistry were prefixed overnight at 4°C in 4% N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDAC, Sigma–Aldrich E6383). Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 50 µM 5-Iodo-2’-deoxyuridine (IdU, Sigma-Aldrich, CAS 54-42-2) and 5 µM SN-38 for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... treated with poly-d-lysine(Sigma-Aldrich A-003-E, 1-2 hr at 37°C, 2 μL/well) followed by laminin (Thermo Fisher 23017015 ...
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bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
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bioRxiv - Cell Biology 2023Quote: ... N=4 (2 females, 2 males) mice were given 12.5 mg/kg sodium nitrite (52mg/L, 23713, Sigma) in 10% blackcurrant squash (Robinsons) ...
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bioRxiv - Immunology 2023Quote: ... human peripheral blood-derived cells were stained with 7-aminoactinomycin D (7-AAD, Sigma) or LIVE/DEAD® viability markers (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Total RNA of the macrophages was extracted by the phenol/1-bromo-3-chloropropane method (Sigma-Aldrich, UK) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... tubes were filled and replenished with a solution of 1 x 10-5 M abscisic acid (2-cis,4-trans-Abscisic Acid, 98%; Sigma-Aldrich; St. Louis MO, USA; product 862169). For the FC experiment ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Cell Biology 2022Quote: ... Cre-mediated recombination was induced in embryos by intraperitoneal injection of 0.5 mg (Cdh5-CreERT2) or 2 mg (Vegfr1-CreERT2, Vegfr3-CreERT2) of 4-hydroxytamoxifen (4-OHT, H7904, Sigma), dissolved in peanut oil (10 mg/ml) ...
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bioRxiv - Immunology 2019Quote: ... mouse peritoneal neutrophils were harvested 4 h after intraperitoneal injection of 2 ml of 4% thioglycollate broth (Sigma-Aldrich). The collected peritoneal neutrophils were cultured in RPMI 1640 (Welgene ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 5-iodo-2′-deoxyuridine (IdU; both from Sigma-Aldrich), 25μM for 60 min ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM βME supplemented with 5 units/ml TurboNuclease (Sigma, T4330)) and lysed by sonication ...
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bioRxiv - Cell Biology 2019Quote: ... mouse Tubulin (clone B-5-1-2 from Sigma, 1:5000), rabbit ZW10 (ab21582 from abcam ...
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bioRxiv - Bioengineering 2021Quote: ... We included 200 μM 5-fluoro-2’-deoxyuridine (floxuridine, Sigma Aldrich) in the media to prevent growth of progeny ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were immersed 2 x 5 min in xylene (534056, Sigma) and hydrated for 5 min in 99 % ethanol (100983 ...
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bioRxiv - Cell Biology 2021Quote: ... SV40 T Antigen (Ab-2) (Merck Millipore; DP02; 5 μg/ml). Secondary antibodies (all from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Doxycycline water (5% sucrose with 2 mg/ml of doxycycline (Sigma) was provided to DDX3X knockdown group to induce knockdown of DDX3X from 1 week after cell injection ...
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bioRxiv - Cell Biology 2021Quote: ... (2) 30 μL 5% pluronic F-127 (Sigma-Aldrich, P2443-250G) incubated for 5 min ...
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bioRxiv - Biochemistry 2022Quote: ... brought to volume with 5% 2-hydroxypropoyl-β-cyclodextrin (Sigma-Aldrich) in water (Mediatech Inc. ...
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bioRxiv - Molecular Biology 2019Quote: ... Decitabine (DAC, 5′-Aza-2’-deoxycytidine) was purchased from Sigma-Aldrich, diluted in DMSO and added 12 hours after plating at 0.01 μM ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of 2 µg/ml Mushroom Tyrosinase (Sigma, Product #T382) and 20 µl of 20 mM L-DOPA are added to the MBTH solution ...
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bioRxiv - Cell Biology 2020Quote: ... reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich), and alkylated with 55 mM 2-Chloroacetamide (Fluka Analytical) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM L-glutamine and polybrene (5 μg/mL, Sigma Aldrich). The following day ...
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bioRxiv - Microbiology 2020Quote: ... Anti-alpha tubulin mAb (clone B-5-1-2; Sigma-Aldrich) and sheep anti-NGF pAb (EMD Millipore ...
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bioRxiv - Developmental Biology 2022Quote: ... In experiments in which 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) was used ...
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bioRxiv - Microbiology 2022Quote: ... 3-Methyl-1-phenyl-2- pyrazolin-5-one (Edaravone; Millipore 443300) was resuspended in ethanol at 500mM and heated at 65°C for solubilization ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with 2 mM 5-FU (Sigma-Aldrich, F5130) for specified time periods before harvest ...
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bioRxiv - Biophysics 2022Quote: ... 1 mM 2-mercaptoethanol) containing 5 μM retinoic acid (Sigma, R2625) and 2 mg/ml doxycycline (TargetMol ...