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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 6 Nitropyridine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... with 1.5% agar supplemented with 2 mg/mL 5-Fluoroorotic acid (US Biological, USA) and 5 µg/mL uracil (Sigma-Aldrich USA). Confirmation of plasmid excision was made by negative selection in BHIS-15% thiamphenicol plates ...
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bioRxiv - Microbiology 2022Quote: ... the medium was supplemented with a mixture of 3 branched-chain fatty acid precursors (100 μm of 2-methyl-butyrate, isobutyrate and isovalerate, Sigma-Aldrich) or straight fatty acid precursors (100 μm of methyl-butyrate ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 CHO cells were seeded per well in a 24-well plate in supplemented Minimal Essential Medium (MEM with 10 % FBS, 2 % non-essential amino acid solution (Sigma Aldrich), 1 % GlutaMAX-1 ...
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bioRxiv - Microbiology 2023Quote: Cross-linking experiments were performed by incubation of 2 mM and 5 mM of suberic-bis-acid ester (3-sulfo-N-hydroxyuccinimy (BS3, Sigma-Aldrich) with the purified recombinant protein in 25 mM sodium phosphate pH 7.4 for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted PRP was resuspended in PBS containing prostaglandin E1 (2 μM, Absin) and ethylene diamine tetra acetic acid (EDTA) (5 mM, Sigma-Aldrich) to prevent platelet activation ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μL of supernatant was incubated with 7.5 mM Tris buffer (pH 8.0) containing 50 μM DTNB (5,5′-dithiobis-(2-nitrobenzoic acid)) (Sigma‒Aldrich, MI, United States), 300 μM acetyl-CoA and 1 mM oxaloacetate ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... a fetal liver lobe was collected in fluorescence cell sorting (FACS) buffer (1x DPBS with 2 % 100 mM Ethylenediaminetetraacetic acid (EDTA, Sigma-Aldrich), 0.5 % BSA ...
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bioRxiv - Plant Biology 2023Quote: ... Flash-frozen plant samples (0.2 g) were ground and extracted using 2 mL of 3 % 5-sulfosalicylic acid (Sigma, ON, Canada). The extract was centrifuged for 10 min at 8050 x g at 4 °C ...
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bioRxiv - Physiology 2024Quote: Dissected intrascapular BAT and inguinal adipose tissues were incubated in high-glucose DMEM containing 2% fatty acid-free BSA (Sigma, A8806) for 30 minutes at 37C ...
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bioRxiv - Synthetic Biology 2023Quote: ... in which strains were grown in Synthetic Complete (SC) media with 2% (w/v) glucose using appropriate amino acid dropout mixes (Sigma-Aldrich) and/or with the addition of antibiotic nourseothricin (100 mg/L ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Neuroscience 2024Quote: ... A commercially available standardized amino acid mixture was utilized to generate a calibration curve for amino acids (Amino acid standards, physiological, Sigma, Nr. A9906-10ML). Calibration curves for all other metabolites were prepared from individual stock solutions prepared in house ...
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bioRxiv - Cell Biology 2020Quote: ... Amino acid restimulation was performed by adding MEM Amino Acids (50x) solution (Sigma Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... formic acid (FA) and trifluoroacetic acid (TFA) were obtained from Sigma Aldrich (Steinheim, Germany). Methanol (MeOH ...
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bioRxiv - Plant Biology 2021Quote: ... linoleic acid and linolenic acid were obtained from Sigma Aldrich (St. Louis, MO, USA).
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bioRxiv - Immunology 2020Quote: ... 10 μM lysophosphatidic acid (LPA) or 10 mM kynurenic acid (KYNA) (both Sigma-Aldrich). Zaprinast (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... and N2 acrylic acid (0.1% w/v, acrylic acid-N-hydroxysuccinimide ester, A8060, Sigma) were successively added and mixed by vortexing ...
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bioRxiv - Developmental Biology 2019Quote: ... Free fatty acids (Free Fatty Acid Quantitation Kit, Sigma-Aldrich, St. Louis, MO, USA) were measured in maternal erythrocytes pooled for each dietary group due to the small volume of erythrocytes available from each animal making individual animal measures impossible.
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bioRxiv - Synthetic Biology 2020Quote: ... nicotinic acid and amino acids were verified with available standards (purity 99%, Sigma-Aldrich). Peak areas were extracted with Thermo Xcalibur (Version 2.2 SP1 ...
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bioRxiv - Molecular Biology 2020Quote: ... All slides were oxidized in 0.5% periodic acid solution (0.5g periodic acid (Sigma-Aldrich) in 100mL of distilled water ...
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bioRxiv - Biophysics 2023Quote: ... A 154 mg of PCA (Protocatechuic acid/ 3,4-Dihydroxybenzoic acid; Sigma #37580-100G-F) was dissolved in 10 ml of HPLC grade water adjusted to pH 9.0 using 1 M NaOH (SRL #96311 ...
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bioRxiv - Microbiology 2023Quote: ... and 6.2 mL of volatile fatty acids solution (2.11 mL acetic acid (Sigma-Aldrich), 0.74 mL propionic acid (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.1% (w/v) acrylic acid-N-hydroxysuccinimide ester (N2 acrylic acid, A8060, Sigma), as previously reported (Prahl ...
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bioRxiv - Bioengineering 2024Quote: ... and 0.1% (w/v) acrylic acid-N-hydroxysuccinimide ester (N2 acrylic acid, A8060, Sigma), as previously reported (110) ...
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bioRxiv - Cell Biology 2024Quote: Periodic acid-Schiff (PAS) (periodic acid, Merck-Millipore, 100524002, Schiff’s reagent, Sigma- Aldrich, 3952016) staining was used to identify glycogen storage within hepatocytes ...
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bioRxiv - Neuroscience 2022Quote: Sympathetic nervous ablation was performed with 6-hydroxydopamine hydrobromide (6-OHDA, Sigma, H116). Universal ablation of sympathetic nerves was performed in mice by i.p ...
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bioRxiv - Plant Biology 2023Quote: ... plants were sprayed with 100 μM 6-benzyl purine (6-BAP, Sigma-Aldrich) or Mock solution 24h prior to analysis ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Microbiology 2020Quote: ... Tissues submerged in RNAlater solution were mixed with 2 ml of 6% (w/v) betaine (Sigma Aldrich, St. Louis, MO, USA) and placed on ice prior to crushing with mortar and pestle until gut homogenates were formed ...
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bioRxiv - Cancer Biology 2022Quote: ... A21111) Slides were counterstained using 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS, Sigma-Aldrich, cat. D9542-5MG). Coverslips were mounted using mounting medium (Fluoromount-G™ Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 1x PBS and fixed on slide with methanol followed by DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich) to stain nuclear and kinetoplast DNA and analysed immediately ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed in methanol and then stained with DAPI (4’,6-diamidino-2-phenylindole) (1 μg/ml) in PBS containing 10 μg/ml of RNase A (Sigma-Aldrich). Cells were visualized using a Olympus IX 81 microscope with FV1000 confocal system using a 100X oil immersion Plan Apo (N.A ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Immunology 2021Quote: ... the slips were washed with PBS three times and stained with DAPI (4’, 6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated in 500 ng/ml 4’,6-diamidino-2-phenylindole (DAPI, diluted in 0.1 M PB, Sigma, 10 min) prior to mounting using Prolong Gold (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... the gels were washed 3 x 20 min with 3.5 ml of ultrapure water and selected specimens were also incubated for 30 min at room temperature with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) (Sigma, D9542) or 10 µg/ml Hoechst 33342 (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... glycans were co-crystallized with a solution of 50% acetonitrile/50% 20 mM ammonium citrate containing 134 mM of 2’,4’,6’-Trihydroxyacetophenone monohydrate (Sigma-Aldrich). MALDI-ToF MS analysis of the N-glycans was performed in the reflector positive and negative ion mode using a 4800 Plus MALDI ToF/ToF Analyzer (AB Sciex ...
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bioRxiv - Synthetic Biology 2021Quote: ... a freshly prepared solution of 5-(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; Sigma-Aldrich Co., St. Louis, MO, USA) in DMSO ...
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bioRxiv - Systems Biology 2020Quote: ... beads were recovered from the emulsion by washing with 2 × 30 mL 6 × saline sodium citrate buffer (diluted from Sigma #S6639) buffer in a 5 µm ÜberStrainer (pluriSelect) ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with DAPI (4’,6’-diamidino-2-phénylindole) and slides mounted with Fluoromount Aqueous Mounting Medium (F4680, Sigma Aldrich, France) before analysis using Zeiss LSM microscope confocal.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were diluted in PBS and sections were incubated for 1 hour at room temperature with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for nuclei visualization ...
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bioRxiv - Bioengineering 2022Quote: ... the tissues were incubated overnight at RT and in dark conditions with a 1/400 dilution of Myosin 4 Monoclonal Antibody (MF20; eBioscience) as primary antibody and 4′,6-diamindino-2-phenylindole (DAPI; Sigma-Aldrich) at 1:5000 ...
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bioRxiv - Cell Biology 2022Quote: The neuroprotective effect of CA against 6-OHDA was evaluated by MTT reagent (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) (Sigma Aldrich, UK, M5655) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... Behaviour was monitored at 500 fps in 6 dpf larvae with their head restrained in 2% low-melting point agarose (Sigma-Aldrich) and their tail free to move ...
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bioRxiv - Genomics 2020Quote: ... The lentivirus containing the pLenti-FNLS-P2A-Puro or pLenti-ABERA-P2A-Puro or desired gRNAs were added to the 0.5 million of HUDEP-2 or HUDEP-2 stable cell line in a one well of the six-well plate along with 6 μg/ml Polybrene (Sigma-Aldrich) and 1% HEPES 1M buffer (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS to stain nuclei ...