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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 6 Hydroxy 2 4 5 triaminopyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Cultures were grown in 6-well plates containing 40 sterile 5 mm glass beads per well (Sigma, 18406). Two replicates were set up ...
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bioRxiv - Cell Biology 2019Quote: ... 6 × 104 cells grown on glass coverslips for 24 h were labelled with 5 µM SBFI-AM (Sigma) and 0.1 % v/v Pluronic F-127 (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... The symplasmic tracer 5(6)-carboxyfluorescein diacetate (CFDA; 50 mg mL-1 in dimethyl sulfoxide [DMSO]; Sigma, USA) was diluted with sterile water to 500 μg mL-1 to generate a working solution ...
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bioRxiv - Immunology 2022Quote: ... was added to a suspension of 5-nitroso-6-(D-ribitylamino)uracil (20.4 mg, 0.069 mmol) in sterile water (1.5 mL, Sigma-Aldrich). The mixture was briefly sonicated until the nitrosouracil dissolved and a colourless solution was obtained59.
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bioRxiv - Developmental Biology 2023Quote: ... Frozen-thawed human blastocysts (5-6 days post-fertilization) were gently treated by acidic Tyrode’s solution (Sigma, T1788) to remove the zona pellucida 4 ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (w/v) sucrose] supplemented with 100 µM concentration of the membrane-impermeant fluorophore 5(6)-carboxyfluorescein (Sigma) at 45 °C to form liposomes with a final lipid concentration of 8 mM ...
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bioRxiv - Cell Biology 2023Quote: ... 6-Diazo-5-oxo-L-norleucine (DON) was used at final concentration of 2,5µM (Sigma Aldrich, ref. D2141). 2-Desoxy-D-glucose (2-DG ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at room temperature or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting pellets were further extracted by sonication in 3X volumes of SDS lysis buffer (50 mM Tris-HCl pH 7.4, 2% SDS, 1X EDTA-free Complete protease inhibitor cocktail [Roche] and 1X phosphatase inhibitor cocktail 2 and 3 [Sigma]). Samples were centrifuged at 21,000g for 30 min at 25°C to obtain the Triton-insoluble (SDS-soluble ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Developmental Biology 2023Quote: Adult zebrafish were injected intraperitoneally at 2 dpi with 20 μl of 2.5 mg/ml of 5-Bromo-2-deoxyuridine (BrdU, B5002-1G, Sigma). Livers were collected and processed for analysis at 5 dpci ...
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bioRxiv - Plant Biology 2020Quote: ... Electrodes with fine tips (Resistance = 4 to 6 GΩ) were filled with ion-selective ionophore cocktails (Sigma, Buchs, Switzerland) and their tips were moved towards and away from the sample in a slow (5 s cycle ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
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bioRxiv - Microbiology 2020Quote: ... 4 mM stable glutamine without sodium pyruvate (Biochrom) and supplemented with 6 %inactivated fetal calf serum (iFCS) (Sigma-Aldrich). HeLa cells were cultured in DMEM containing 4.5 g × l−1 glucose ...
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bioRxiv - Microbiology 2023Quote: ... β-mercaptoethanol (99%) for 6 h at 4 ℃. The proteins were concentrated by Amicon Ultra (M.W. 3,000 Da) (Sigma-Aldrich) and stored at −80 ℃.
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bioRxiv - Cell Biology 2023Quote: ... we removed the zona pellucida by washing the oocytes in 4-6 drops of acidic Tyrode’s solution (Millipore Sigma) and rinsed them in 4-6 drops of CZB media with the corresponding drug(s ...
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bioRxiv - Plant Biology 2023Quote: ... spy-3 and spy-4 were sprayed with either 50 µM or 100 µM BA (6-benzylaminoadenine, Sigma B3408) or mock [200 mM NaOH] three times ...
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bioRxiv - Physiology 2023Quote: ... Unspecific antibody binding was blocked with 6% donkey serum or 4% goat serum (Sigma-Aldrich, St Louis, MO, USA), and 2.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.5 mM DTT) and concentrated to ∼ 5 mg/mL (Amicon Ultra 4 mL Centrifuge filters, 30 KDa NMWL devices, Millipore Cat# UFC805024). Last ...
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bioRxiv - Cell Biology 2023Quote: ... Then cells were incubated for 6 h at 37 °C under a humidified atmosphere with 5% CO2 in the presence of 5 μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (Sigma-Aldrich, USA) to induce mitophagy ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transduced with 5 μl (2× 108 IU/μl) viral solution together with 5 μg/ml polybrene (EMD Millipore) for 5-7 days ...
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bioRxiv - Neuroscience 2022Quote: ... 5-16-day old females were fed sheep blood supplemented with 2 mM adenosine 5’-triphosphate (ATP) (Sigma Aldrich, A6419) in aqueous sodium bicarbonate buffer using a new artificial membrane feeder called the blood puck ...
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bioRxiv - Neuroscience 2022Quote: ... Cohort 2: 10 (5 male and 5 female) received a single daily injection of BrdU (150 mg/kg, i.p.; Sigma B5002) on days 6 – 8 after infusion and 3 injections of EdU on day 21 after lentivirus injection ...
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bioRxiv - Cell Biology 2022Quote: ... All samples with SDS sample buffer were heated at 95°C for 5 minutes and reduced with 5% 2-mercaptoethanol (Sigma) as needed.
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions (some of which were pooled from tumors harvested from 2-3 mice) were stimulated for 5 h with PMA (5 ng/ml, Sigma) and ionomycin (500 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were denatured for 5 min at 95 °C in Bolt LDS Sample buffer with 5 % v/v 2-mercaptoethanol (Sigma). 10-30 μg of proteins were loaded in Bolt 4-12 % Bis-Tris gels (Thermo ...
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bioRxiv - Biophysics 2023Quote: ... 10 µL of the lipid solution was added to 89.5 µL H2O containing NaCl and 0.5 µL of a 5% solids solution of 2 µm silica microparticles (Sigma Aldrich) to a final concentration of 0.1 mM lipids and 1 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then dehydrated by 2x 5 min 100% ethanol washes and cleared with xylol (2 x 5 mins) before mounting with Entellan (Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... the following doses of chemicals and genotoxic agents were used: 5-Aza-2’-deoxycytidine (5-AzadC; 10 μM, Sigma-Aldrich), 5-Ethynyl-2’-deoxyuridine (EdU ...
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bioRxiv - Immunology 2019Quote: ... the stock solution of 6-hydroxydopamine (6-OHDA) (Sigma) was prepared in water at 42 mg/mL and stored at −20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with interleukin-6 (IL-6, Sigma) for 24 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA (10mg/ml; 6-OHDA hydrobromide, Sigma-Aldrich) was dissolved in a vehicle solution immediately before the surgeries ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilized cells were blocked with 5% BSA diluted in PBS for 4 h at 4°C and incubated overnight with pan-Cadherin antibody (Sigma, C1821, 1:500), MRP2 antibody (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples (5 μg) were used to determine GCase activity upon addition of 10 mM 4-Methylumbelliferyl ß-glucophyranoside (4-MU, Sigma-Aldrich, # M3633) in 1% BSA with or without 2 mM conduritol-b-epoxide (CBE ...
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bioRxiv - Molecular Biology 2021Quote: ... and Glucose-6-phosphate dehydrogenase (G-6-PDH) with anti-G-6-PDH antibody (Sigma A9521). Blots were probed with IgG Goat anti-Rabbit (BioRad 170-6515 ...
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Molecular Biology 2020Quote: ... Mice were kept on 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. No. PHR1731-1G) in drinking water when indicated ...
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bioRxiv - Neuroscience 2023Quote: ... male (N = 2) and female (N = 2) naïve mice were infused with Fluorogold (Sigma, St. Louis, MO; 4% in sterile saline) in the DMS (0.2 µL) ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min and blocked for 2 hours at 25 °C or overnight at 4 °C using 2% BSA (Sigma-Aldrich). Samples were incubated with respective primary antibodies at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... were reared in 10g/l of glucose/200µM of 3BDO (3-Benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one) (Sigma Aldrich) supplemented in standard fly food ...
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bioRxiv - Neuroscience 2020Quote: ... Tubes were placed in a cylindrical phantom (2 cm diameter, 2 % agarose mixed with 5 % intralipid (Sigma-Aldrich AG, Switzerland). The phantom holder was placed in an imaging chamber filled with water and kept at 28°C.
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bioRxiv - Neuroscience 2023Quote: The mice received three injections every 2 h (200 ul/injection, intraperitoneally in alternative sites of the body) of 5-Chloro-2’-deoxy-Uridine (CldU, Sigma reference C6891 ...
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bioRxiv - Neuroscience 2021Quote: A solution containing 10 μM of 5-bromo-2’-deoxyuridine (BrdU; SIGMA #858811) was added to the growth medium of SK-N-SH cell monolayers and cells were incubated at 37°C for 2 hours to estimate the S-phase index ...
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bioRxiv - Biophysics 2020Quote: ... 10 mM NaN3 and 5 mM 2-deoxy-D-glucose (Sigma-Aldrich, Singapore).
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bioRxiv - Cell Biology 2022Quote: ... (B-5-1-2 coupled to Alexa-488, Thermo Fisher or DM1A, Sigma), rat anti-tyrosinated tubulin (YL1/2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5-aza-2’-deoxycytidine was purchased from Sigma-Aldrich (Cat. A3656, Louis, USA).
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bioRxiv - Neuroscience 2019Quote: ... and anti-α-Tubulin (mouse monoclonal [B-5-1-2], 1:8,000, Sigma).