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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 5 Chloro 1 vinyl 2 pyridone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... mice received injections of tamoxifen (2 mg per day for either 5 or 7 days, Sigma-Aldrich, T5648) or an equivalent volume of corn oil as a vehicle control ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 150 mM NaCl and 5% (vol/vol) glycerol supplemented with 1mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma). Elution fractions were collected and evaluated by SDS-PAGE and relevant fractions with purified proteins were concentrated as appropriate for further studies.
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bioRxiv - Biophysics 2021Quote: ... Microcrystals 1-5 × 5-10 × 10-20 μm3 in size were passed through 100 micron plastic mesh filters (Millipore, USA) in the same mother liquor composition to eliminate the large single crystals and other impurities before the data collection ...
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bioRxiv - Biophysics 2020Quote: ... Microcrystals 1-5 × 5-10 × 10-20 μm3 in size were passed through 100 micron plastic mesh filters (Millipore, USA) in the same mother liquor composition to eliminate the large single crystals and other impurities before the data collection ...
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bioRxiv - Neuroscience 2020Quote: ... the iN cells were dissociated with Accutase and replated with mouse glia cells on Matrigel-coated coverslips in 24-well plate (150k cells/well) in Neurobasal 1% serum medium containing 5 μM 5-fluorodexoyuridine (FUDR, Sigma) and 4 μM Uridine (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG ...
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bioRxiv - Cancer Biology 2024Quote: ... 4T1 cell lines were provided by WSU Animal Model and Therapeutics Evaluation Core (AMTEC) and were grown in 5% CO2 using DMEM supplemented with 1% penicillin-streptomycin and 5% fetal bovine serum (Sigma) as media at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid) was purchased from EMD Millipore (Billerica, MA USA) and NaCl (sodium chloride ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5 µL g-1 solution of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) was added at 4°C temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM lipids: POPC: POPE: cardiolipin with a 2:2:1 weight ratio) and 160 μL MSP1D1 (Sigma, 5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... TEBP-1-His5 and TEBP-2-His5 were expressed in Rosetta 2 (DE3) pLysS Competent Cells (Novagen,#71401). An overnight culture was grown in LB containing the respective antibiotic ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 μL g−1 hydrogel of the photoinitiator 2-hydroxy-2-methyl propiophenone (Irgacure 1173; >97 %, Sigma-Aldrich) were mixed in at a temperature of 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 150 μM (2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5 compound; Sigma-Aldrich, EF5014), 0.5 μM MitoSOX™ fluorescent probe (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: Metabolic quenching and nucleotide phosphate metabolite pool extraction were performed by adding 1 ml ice-cold 2:2:1 acetonitrile:methanol:water with 0.1% formic acid with 1x MS-Stop phosphatase inhibitor (Sigma-Aldrich). Samples were spiked with 10 µl 100 mM mix of adenosine-15N5-5′-triphosphate ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg ml-1 of 3–5 kDa fluorescein isothio-cyanate (FITC)-dextran (Sigma-Aldrich, Germany) in phenol-red free DMEM/F12 medium (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... and 5 U ml−1 penicillin and 50 μg ml−1 streptomycin (Merck Life Science (Sigma)) at 37 °C and 5% CO2 ...
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bioRxiv - Physiology 2020Quote: ... diluted with dH2O (1:5) and BSA (bovine serum albumin, 1 mg/ml) (Sigma Chemicals, USA) as a standard ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were infected with pLKO.1 control or pLKO.1-shZSCAN4 (5’-GAATGCAACAACTCTTGTAATCTCGAGATTACAAGAGTTGTTGCATTCT-3’, Millipore Sigma) and further selected with Puromycin (1ug/ml ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... and blocked (1 h, 37°C, 5% dry milk in TBST or 1% BSA (Sigma, #A7906)) ...
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bioRxiv - Developmental Biology 2021Quote: ... and two 5 min washes in 100% methanol and clearing in 1:1 v/v methanol/BA:BB (2:1 benzyl alcohol:benzyl benzoate; Sigma), and two washes in 100% BA:BB ...
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bioRxiv - Bioengineering 2022Quote: ... the chemostat feed contained 0.25 g L-1 or 2 g L-1 ammonium chloride (equal to 0.07 or 0.9 g L-1 ammonium) (Sigma) for ammonium- or cellobiose-limited cultivations ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were picked up using a 1:1 mixture of 2% methyl cellulose (Sigma) and 2.3M sucrose ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg pSPAX2 and 1 µg pMD2.G with 1 mg/mL polyethyenimine (PEI, Sigma) in serum-free DMEM ...
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bioRxiv - Genetics 2023Quote: ... 1 µg ml–1 natural mouse laminin and 2 µM arabinosylcytosine (Ara-C, Sigma-Aldrich). After 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... the fish were anesthetized or euthanized using 1:1000 or 1:500 2-phenoxyethanol (Sigma), respectively.
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bioRxiv - Cell Biology 2023Quote: ... A total of 2 ml of washing solution 1 (WS1: 1X PBS, 1% (BSA, Sigma), 0.1% Triton X ...
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bioRxiv - Neuroscience 2024Quote: ... tissue supernatant and serum were incubated 1:1 in 2% Trichloroacetic acid (Millipore Sigma: T9159) overnight (4°C) ...
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bioRxiv - Microbiology 2024Quote: ... rabbit anti-caveolin-1 (1:1,000, 2 hours at room temperature, catalog no. C4490; Sigma), mouse anti-E-cadherin (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM DTT, 1 mM EGTA, 2 mM NaF, 1 μM microcystin, 1 mM benzamidine, 1 mM sodium orthovanadate, and Sigma-Aldrich protease and phosphatase inhibitor cocktails) ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... and then modified with 1:1 volume ratio with 1 mg/ml biotin-5-fluorescein conjugate (Sigma-Aldrich, cat #53608) followed by three washes with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and then with Theobroma cacao extract fraction 5 (100 µg.mL-1) or (-)-epicatechin (80 µg.mL-1) (Sigma-Aldrich, HW101708-1) for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... 10 mM NaPO4 buffer, 5 mM EDTA, 50 % deionised formamide, 1 mg ml-1 tRNA, 1 x Denhardt’s solution [Sigma-Aldrich, D2532] ...
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bioRxiv - Plant Biology 2024Quote: ... 50 μL of extraction buffer (100 mM Tris pH 7.5, 150 mM NaCl, 10 % [v/v] glycerol, 10 mM EDTA, 1 mM DTT, 1 mM PMSF, 1 mM Sigma protease inhibitor and 1 % [v/v] IPEGAL CA-630 ...
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bioRxiv - Molecular Biology 2020Quote: ... The pellets were resuspended at an OD600 of 1 in 5 ml of agroinfiltration buffer (10 mM MgCl2 and 250 μM of 3’,5’-Dimethoxy-4’-hydroxyacetophenone (Sigma-Aldrich)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked for a minimum of 1 hour at room temperature in 5% (w/v) non-fat milk or 5% (w/v) BSA (A4503-50G; Sigma Aldrich) in TBS-T (1x TBS and 0.1% Tween-20 ...
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bioRxiv - Biochemistry 2020Quote: ... HLCs were exposed to either 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate (AICAR; 1mM; Sigma-Aldrich, A1393-50MG), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl cells were spread on a 1 mm glass slide pre-treated with 5 μl poly-L-lysine (Sigma Aldrich). A Nikon 9Oi eclipse microscope was used to capture differential interference contrast (DIC ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were split in two and either 5 µL buffer or 5 µL 1 µg/µL Proteinase K (Millipore Sigma 3115887001) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked using 5% nonfat milk for 30 minutes and incubated overnight in 5% milk with the following antibodies: PHGDH (1:1000, Sigma HPA021241), GAPDH (1:5000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed 5-6x for 5 min with water and then rinsed in 1% Phosphomolybdic acid (Sigma-Aldrich, HT153) for 10 min and rinsed in water for 5 min ...
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bioRxiv - Neuroscience 2023Quote: Medium was exchanged with a 5:1 mixture of pre-warmed culture medium and 5 mg/ml MTT (Sigma-Aldrich, M5655) in PBS for a total volume of 120 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 hour at room temperature in 5% (w/v) non-fat milk (Marvel) or 5% (w/v) BSA (Sigma-Aldrich) depending on the antibody specifications ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 10 min at room temperature or directly washed by centrifugation at 5,000×g for 5 min and resuspension in 5 µl of 1×PBS supplemented with 0.2 µg/ml propidium iodide (PI) (Sigma-Aldrich P4170). Cells were placed on a 1% agarose pad that was supplemented with either 0.2% arabinose or both 0.2% arabinose and 0.1 mM IPTG ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized with 0.25%Triton X-100/PBS for 15 min and blocked for 1 h at RT with 5% NGS (Jackson Immuno Research, 005-000-121) + 5% BSA (Sigma, A2153). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 5 units/μl of DNA polymerase in diluted Polymerase Buffer with Red Fluorescent-labeled Oligonucleotides (1:5, Sigma Aldrich) for 100 minutes at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and the recorded transients were characterized using the same software.65,66 Electrodes were calibrated using background-subtracted cyclic voltammograms obtained with 1 μM 5-HT solution diluted in PBS (5-HT; Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...