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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 3' Methyl 1 1' biphenyl 4 amine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Genetics 2020Quote: ... and the colour reaction was carried out with NBT/BCIP (4-nitroblue tetrazolium chloride/bromo-4-chloro-3-indolyl phosphate; Sigma). Sections were dehydrated and mounted with Vectamount (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Systems Biology 2024Quote: ... and kept at 4°C for a maximum of 4 hours until plasma was separated via centrifugation (2600g for 10 minutes at 4°C) (Sigma 3-16KL, Sigma Laboratory Centrifuges ...
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bioRxiv - Plant Biology 2024Quote: ... AGPs were finally detected using the following substrates: 4 mg of 5-bromo-4-chloro-3-indolylphosphate (BCiP; Sigma, USA) in 1 mL water and 9 mg of nitro-blue tetrazolium (NBT ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Biophysics 2020Quote: ... passaged every 3-4 days by trypsinization with trypsin-EDTA solution (Sigma-Aldrich) and moved to the corresponding Petri dishes ...
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bioRxiv - Plant Biology 2021Quote: Starting reagents 3-hydroxybenzaldehyde 4 and hydrazine hydrate were purchased from Sigma-Aldrich and phenylacetic acid 1 was purchased from Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... noggin and EGF and containing 10 µM nutlin-3 (Sigma #675576-98-4).
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bioRxiv - Neuroscience 2020Quote: ... The odorants used were 0.1% 3-octanol and 0.1% 4-methylcyclohexanol (Sigma Aldrich), controlled via three-way solenoids (Lee Company) ...
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bioRxiv - Immunology 2021Quote: ... and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (Sigma-Aldrich). Air-dried plates were read ...
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bioRxiv - Developmental Biology 2023Quote: ... and blocked overnight at 4°C in DPBS with 3% BSA (Sigma, A9647) and 0.05% TritonX-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×10-4 M monothioglycerol and 300 μg/ml human transferrin (Sigma Aldrich). Cells were plated in petri dishes (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... and used without further purification: 4-Hydroxy-3-methoxycinnamaldehyde (458-36-6, Sigma), 3-ethoxy-4-hydroxybenzaldehyde (121-32-4 ...
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bioRxiv - Bioengineering 2023Quote: ... For ECM conjugation using L-DOPA (3, 4- Dihydroxy-L-phenylalanine, D9628, SIGMA),[23] 2 mg ml-1 L-DOPA solution was prepared using 10 mM Tris-HCL buffer (pH balanced to 10.2 using 1 M NaOH ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were transferred to a 4% solution of (3-aminopropyl) triethoxysilane (Sigma #440140) in acetone for one hour ...
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bioRxiv - Microbiology 2024Quote: ... 4°C with 3 K Amicon® Ultra-15 centrifugal filters (Merck Millipore). All fractions were stored as aliquots at − 80 °C for later use ...
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bioRxiv - Neuroscience 2024Quote: ... followed by perfusion with 4% paraformaldehyde (PFA) for 3 minutes (Sigma-Aldrich, USA) dissolved in 0.1 M Phosphate Saline Buffer (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... and 5-Bromo-4-chloro-3-indolyl phosphate-toluoidine were from Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-adducin phospho S355 antibody (Abmart 20592-1NB-3/C63-S, 1:100, for WB and double transgenics) anti-FLAG antibody (M2, F3165, Sigma-Aldrich 1:100). Myosin Light Chain 2 antibody (ptglab ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was exchanged with 50 μL of fresh medium with 1 mM 3-isobutyl-1-methylxanthine (IBMX) (Sigma-Aldrich, Cat# I7018-100MG), and cells were incubated at 37°C for an additional 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168 ...
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bioRxiv - Neuroscience 2022Quote: ... CAS# 83-34-1), indole (98%, CAS# 120-72- 9), and 3-ethylindole (97%, CAS# 1484-19-1) were provided by Sigma-Aldrich (Milwaukee, WI), ACROS Organics (Geel ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were extracted and post-fixed for at least 1 day in 4% PFA at 4°C and transferred to 30% sucrose (Millipore Sigma) for at least 1 day at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.4) for 1 hour at room temperature and then incubated at 4°C overnight with primary antibodies (anti-multicillin, Sigma Aldrich, HPA058073 1:500 ...
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bioRxiv - Plant Biology 2020Quote: Spin trapping assays with the spin probe 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN; Sigma-Aldrich, St. Louis, USA) to detect the formation of hydroxyl radicals24 were carried out using cyanobacterial cells (OD730 = 0.65 ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged (500g at 4°C for 4 minutes) and resuspended in PBS containing 1 μg/mL DAPI (Sigma Aldrich, D9542-100MG). Analysis of flow cytometric data was preformed using the BD Fortessa X20 and FlowJo v10 software (Ashland) ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed for 1 day in 4% PFA at 4°C before transferring to 30% sucrose (Millipore Sigma) in PBS at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... they were incubated in the primary antibody solution composed of anti-aquaporin 4 (AQP-4) (1:300 dilution in blocking buffer, Millipore Sigma) and anti-LRRC8A antibody (1:200 dilution in blocking buffer ...
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bioRxiv - Bioengineering 2022Quote: Specimens were permeabilized with 4% paraformaldehyde and incubated for 1 hour at room temperature in primary antibody anti-vinculin (1:500, Sigma Aldrich, V4505), secondary antibody Alexa Fluor 488 (1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were incubated overnight at 4°C with primary antibodies, GFP (Novus NB1001770, 1:100) and NeuN (EMD Millipore MAB377, 1:150) in 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162; PAX6 mouse 1:80, DSHB) diluted in solution containing 2.5% NGS and 0.02% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were fixed with 4% PFA and immunostained with mouse anti-HA (Covance, 1:1000) or rabbit anti-Flag (Sigma, 1:1000), followed by incubation with AlexFluor-647-conjugated anti-human Fc γ and species-specific AlexaFluor-488- or -546-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1 h at RT and after washing coverslips were incubated for 4 min with DAPI (1:4000, Sigma-Aldrich #D8417) and mounted with ProLong Gold Antifade reagent (Invitrogen #P10144).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and applied on previously seeded NIH3T3 Flp-In cells at a ratio of 1:1 (vol/vol, supernatant : NIH3T3 growth medium) together with 4 µg/ml Polybrene® (Sigma-Aldrich). Transduced cells were cultured in regular growth medium supplemented with 5 µg/ml blasticidin (Carl Roth GmbH + Co ...
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bioRxiv - Cell Biology 2023Quote: ... cells were harvested on ice and lysed by rotation for 1 h at 4°C in JIES buffer (see above) containing 1× SIGMAFAST protease inhibitor cocktail (Sigma-Aldrich, S8830). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in TBS-T with 5 % non – fat milk for 1 hour in RT following incubation overnight at 4 °C with primary (1:1000) antibodies: Flag (Sigma, Cat. F1804), IL-1β (Cell Signaling Technology ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked in 1% Casein/PBS (1 hour, room temp) and incubated with the following primary antibodies (4°C, overnight): anti-Nup93 (1:1000, Sigma-Aldrich, HPA017937); anti-Nup107 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542) at RT for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies at RT for 2 hours with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542). Afterward ...
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bioRxiv - Molecular Biology 2024Quote: ... and overnight at 4 °C with primary antibody against Nucleolin or NPM1 (Nucleolin - abcam, ab22758 1:1000; NPM1 – Sigma, B0556 1:500). Cells were washed three times with 1X PBT for 5 minutes each and then incubated for 1 h at room temperature with secondary antibody (goat anti-rabbit IgG (H+L ...
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bioRxiv - Genetics 2024Quote: ... in TBS-T at room temperature for 1 h and incubated at 4 °C overnight with mouse-anti FLAG (1:400, Sigma-Aldrich, F3165) or mouse-anti Cas9 (1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...