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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... adding human Apo-transferrin (Sigma, cat.no.T1147), Putrescine dihydrochloride powder ...
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bioRxiv - Biophysics 2024Quote: ... IgM from human serum (Sigma-Aldrich). We prepared the antibody stock solution in 1X PBS (pH 7.2 ...
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bioRxiv - Cell Biology 2024Quote: ... Proliferating AC16 human cardiomyocytes (Sigma-Aldrich SCC109 ...
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bioRxiv - Cell Biology 2024Quote: ... human plasma fibronectin (FC010, Millipore, USA) solution (10 μg/ml in PBS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Human mammary epithelial cells (Sigma-Aldrich) were cultured with Human Mammary Epithelial Cell Growth Medium (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... purified human C1q (10μg/ml; Millipore) or fresh human serum at 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... were coated with human IgM (Sigma) at 1:100 for 1 h at 37°C ...
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bioRxiv - Pathology 2024Quote: ... human anti-FLAG (Sigma, Cat # F1804). Coverslips were placed in 12-mm culture dishes where HEK293 or Neuro2A cells were seeded ...
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bioRxiv - Cell Biology 2024Quote: ... in human serum (P2918; Sigma-Aldrich) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5% human serum (Sigma) at a density of 1.5 x 106 cells/well in a 24-well plate ...
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bioRxiv - Genetics 2024Quote: Human skin fibroblasts (adult, Sigma-Aldrich) were cultured in DMEM (low glucose ...
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bioRxiv - Cell Biology 2024Quote: ... human ZBP1: 5’-CGGTAAATCGTCCATGCTT-3’ (Sigma). The gRNAs were cloned into pSpCas9n(BB)-2A-GFP plasmid (PX461 ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Cell Biology 2020Quote: ... in the presence or the absence of 10 μg N-terminal-His-tagged ubiquitin (Sigma-Aldrich, #U5507), and ≈ 2 μg of purified recombinant mouse GST-Trim39 (WT ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 2% HI-FBS PBS with 1 µg/mL DAPI (Sigma Aldrich, #D9542) to stain dead cells ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant plasmid DNA was transferred to Escherichia coli strain Rosetta 2(DE3)pLysS (Novagen). At least four colonies for each experiment were sequenced to check for errors in the PCR ...
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bioRxiv - Neuroscience 2021Quote: ... Peprotech) and recombinant rat IFNγ (reconstituted in pH 8 sodium phosphate [10mM; Sigma-Aldrich] were diluted to the required concentrations (LTα 1μg + IFNγ 75ng per injection ...
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bioRxiv - Cell Biology 2022Quote: The recombinant GST-tagged Nanobody against GFP69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of recombinant PRDX6 was induced with 1mM isopropyl β-d-thiogalactopyranoside (IPTG, Sigma). The culture was incubated for 20h at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... HMWHA or BCM− was pre-incubated with 300 IU/mL recombinant HYAL2 (Sigma-Aldrich) for 30 min prior to addition to co-cultures.
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bioRxiv - Molecular Biology 2020Quote: ... Digestion was performed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RNase R (30 pmols) or Bovine Serum Albumin (BSA, Sigma-Aldrich, MO, USA) were alone or combined with Csm5 WT or Csm5Δ46 (90 ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Microbiology 2024Quote: ... A luciferase solution containing recombinant luciferase from Photinus pyralis (Sigma-Aldrich, 20 μg/mL), ATP disodium trihydrate (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant murine WNT5a (20 ng/ml, Biotechne) and acetylcholine chloride (100 μM, Sigma Aldrich). Organoids were harvested for downstream analysis on the 7th day after passaging.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 1000 U ESGRO recombinant mouse leukemia inhibitory factor (mLIF; Sigma-Aldrich #ESG1107), on plates coated with growth factor reduced Matrigel (8.7 μg/cm2 ...
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bioRxiv - Bioengineering 2024Quote: ... containing B8 supplemented with 0.8 mg/mL of recombinant albumin (rAlbumin, Sigma Aldrich #A9731)(Stout et al. ...
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bioRxiv - Bioengineering 2022Quote: Human ovarian cancer A2780 and Adriamycin resistant human ovarian cancer A2780-R cells were procured from Sigma Inc ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte- human fibrinogen mixture (Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2023Quote: ... 1µL thrombin from human plasma was added to 3.5µL of the cardiomyocyte-human fibrinogen mixture (Sigma-Aldrich) and 2µL of the mixture is carefully pipetted into the microwell ...
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bioRxiv - Bioengineering 2024Quote: ... the unattached cells were removed using Human Endothelial SFM supplemented with 5% human serum (Sigma, Cat.# P2918) and 10 µM Y27632 ...
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bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
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bioRxiv - Cell Biology 2020Quote: ... 8 µg antibody (Monoclonal ANTI-FLAG® M2, Art. No. F3165, Sigma-Aldrich® / Myc-Tag (9B11) Mouse mAb ...
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bioRxiv - Biochemistry 2022Quote: ... the GST-tag of GFP-N-WASP-VCA and cortactin was cut by PreScission Protease (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies against FLAG tag [M2] (F1804, RRID: AB_262044) and [M2] (F3145, RRID:AB_259529) were purchased from Sigma Aldrich. Antibodies against DDX3X [D19B4] (8192S ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in phosphate-buffered saline (PBS) containing 1/400 anti Rho-tag antibody 4D2 (Millipore), 15mM NaN3 ...
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bioRxiv - Microbiology 2020Quote: ... the mFc and 8xHis tags at the C-terminus were removed by HRV-3C protease (Millipore-Sigma) and then purified using Ni-NTA resin (Invitrogen ...