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Citations for Millipore Sigma :
1551 - 1600 of 2557 citations for PSMD6 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in 50µL of FLAG peptide solution (1µg/µL in RIPA, Sigma-Aldrich, F3290) after the last wash to elute FLAG-tagged Arc and other proteins from beads ...
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bioRxiv - Cell Biology 2021Quote: ... grown for 2.5 hours and arrested by adding 3 μg/ml α-factor (Sigma, custom peptide WHWLQLKPGQPMY) every 60 min for 120 min ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
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bioRxiv - Biochemistry 2021Quote: ... tryptic peptides were acidified to a final concentration of 2% (v/v) with formic acid (Sigma Aldrich) and desalted using StageTips (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... the digested peptides were alkylated with 20 μL iodoacetamide (5 mg/mL) (Life Science, Sigma-Aldrich, GmbH) and incubated for 30 min in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound proteins were eluted using IP buffer containing 0.25 mg/mL of 3x FLAG peptides (Sigma-Aldrich) at 25 °C for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
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bioRxiv - Immunology 2020Quote: ... peptide was dissolved in anhydrous dimethyl sulfoxide (DMSO) at 10mg/ml and anhydrous triethylamine (TEA) (both Sigma) was added stoichiometrically to convert the peptide salts into their free forms in order to deprotonate the primary amine from the lysine side chain ...
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bioRxiv - Neuroscience 2021Quote: ... these cells were cultured in the presence of the OVA257-264 peptide (GenScript RP10611 or Sigma S7951). After 12 hours ...
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bioRxiv - Neuroscience 2021Quote: ... The phosphorylated peptides were eluted from the beads by incubation with 1.5% ammonium hydroxide solution (Sigma-Aldrich), pH 11.3 ...
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bioRxiv - Cell Biology 2021Quote: ... The pellets were resuspended in 250 μL of IP lysis buffer with addition of Flag peptide (Sigma), and the mixture was rocked overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Peptide fractions were incubated on a rotating platform with IMAC beads (PHOS-Select iron affinity gel (Sigma)) for 1hr at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... The FLAG-IP was eluted twice with 300 µl 250 µg/ml 3X FLAG peptides (Sigma, F4799) in TBS (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... Peptide hydrolysis for ecClpX and ecClpP was monitored with the substrate Suc-Leu-Tyr-AMC (Millipore Sigma) under the same conditions ...
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bioRxiv - Genomics 2020Quote: ... Expression of Cas9 was confirmed by Western blot using an anti-2A peptide antibody (Millipore Sigma MABS2005).
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bioRxiv - Systems Biology 2021Quote: ... Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma) were used as external standards for absolute protein quantification (more details in Supp ...
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bioRxiv - Biophysics 2019Quote: ... and eluted with 5 mL of buffer C containing 0.1 mg/mL FLAG peptide (Sigma-Aldrich, F3290). BLM was further purified using a 1 mL HiTrap SP (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... and eluted with 5 mL of buffer C containing 0.1 mg/mL FLAG peptide (Sigma-Aldrich, F3290). DNA2 was further purified using a 1 mL HiTrap SP (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The elutes were combined and the peptides were further purified using C18 ZipTip Pipette Tips (Millipore, USA) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells were cultured with overlapping peptide pools at 2LJμg/ml or PHA (Sigma Aldrich, St Louis, MO) at 10 µg/ml as a positive control for 16-18 hours at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... or eluted by competition with Flag peptide at 200ug/ml (five incubations of 5 minutes, P4799, Sigma). Samples were finally concentrated using 3KDa columns (Millipore).
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bioRxiv - Cell Biology 2021Quote: ... the proteins bound to the beads were eluted by the addition of FLAG peptide (#F3290, Sigma-Aldrich). Proteins in the eluate were separated by SDS-PAGE to detect Tanmp-interacting proteins by Western blotting or by silver staining ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated proteins were eluted with 0.5 mg/mL (diluted in lysis buffer) 3X FLAG peptide (Sigma, F4799) for 30 minutes on a rotator ...
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bioRxiv - Biochemistry 2022Quote: ... bound proteins were eluted with lysis buffer containing 300 μg/ml Flag peptide (Sigma-Aldrich, Cat# F3290) at 4 °C for 45 min ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were separated using a gradient from 92% A (0.1% formic acid, FA, Sigma, in deionized water) and 8% B (0.1% FA in acetonitrile ...
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bioRxiv - Microbiology 2022Quote: ... anti- FLAG M2 magnetic beads were incubated with 100 µg/ml 3x FLAG peptide (Sigma, #F4799- 25MG) in FIB buffer + 10% glycerol for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides from both consecutive digestions were combined and recovered by solid phase extraction using C18 ZipTips (Millipore), eluted in 15 μl 50% acetonitrile 0.1% formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 4 h in presence of SIINFEKL peptide + 10 µg/ml of brefeldin A (Sigma-Aldrich). Specific CD8+ T cells were evaluated by incubation with SIINFEKL257-264-APC-Labeled or SIINFEKL257-264-BV421-Labeled tetramers (NIH Tetramer Core Facility at Emory University) ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were eluted twice (15 min. at RT) by incubation with 3x-FLAG peptide (Sigma #F4799) at a concentration of 100 mg/mL in PBS buffer ...
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bioRxiv - Microbiology 2024Quote: ... was utilized to measure the binding kinetics of three peptides to lipid A (L5399, Sigma, Shanghai, China). Peptides were diluted with 75 mM NaCl solution at concentrations of 100 ...
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bioRxiv - Microbiology 2024Quote: ... Bound material was eluted by incubating the beads with HA peptide (Sigma Aldrich, St. Louis, MO, USA) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore, Cat# ZTC18S096), and resuspended in 0.1% formic acid for analysis by liquid chromatography-mass spectrometry (LC-MS/MS) ...
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bioRxiv - Cell Biology 2023Quote: ... Following primary antibodies were used for IHC and IF: mouse anti-2A peptide (Millipore, MABS2005, 1:200), rabbit anti-Active YAP1 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and each peptide fraction was then cleaned up using 2 ug capacity C18 ZipTips (Millipore, Billerica, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Peptides collected for the DIA spectral library were desalted with ZipTips C18 column (EMD Millipore, Burlington, VT), dried in speedvac and resuspended in 300µl of 0,1% TFA ...
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bioRxiv - Molecular Biology 2023Quote: ... Complexes were eluted with 5 CVs of wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). Eluted protein was analysed by SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... To generate CD8+ T lymphoblasts responsive to ovalbumin peptide (OVAp; SIINFEKL OVA257–264, Cat# S7951, Sigma-Aldrich), T cells were incubated for 48h with OVAp (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... or 790 peptide (Ac-IQAQ ARGQ LMRI EFKK)15 emulsified in complete Freund’s adjuvant (CFA, Sigma-Aldrich) supplemented to 5 mg/ml heat-killed Mycobacterium tuberculosis strain H37Ra (BD Biosciences) ...
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bioRxiv - Biochemistry 2024Quote: ... Bound protein was eluted off agarose gel with 30-50 μL 1X 3XFLAG peptide (Sigma catalog #F4799) at 4 °C with rotation for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... INO80 was eluted by 1 ml of the same buffer with 0.5 mg/ml 3xFLAG peptide (Sigma), followed by 2X 1ml of buffer with 0.25 mg/ml 3xFLAG peptide ...
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bioRxiv - Neuroscience 2024Quote: ... Purified PAK6 was then eluted with in Buffer B supplemented with 150 µg/ml 3xFlag peptides (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... and saturated with blocking solution for 30 min before immune-staining with Duolink by using PLA Technology (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with blocking solution containing 10% normal donkey serum (DNS) and 1% Triton X-100 (Sigma-Aldrich) in PBS with 0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS overnight, incubated with a blocking solution (PBS, with 0.5% Triton X-100 (Sigma-Aldrich) and 10% goat serum (Sigma-Aldrich) ...