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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The polyclonal anti-Hcp antibody was developed after immunization of six-week-old female BALB/c mice with three subcutaneous injections of the purified His-Hcp protein emulsified in Freund adjuvant (Sigma).
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 250,000 g for 30 min and the supernatant was incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Plant Biology 2023Quote: The auto activity of yeast baits on Sc-His due to minimal HIS3 expression was determined by spotting them on Sc-His media containing 0-30 mM 3-Amino-1,2,4-triazole (3-AT) (A8056, Sigma-Aldrich, USA). The minimum concentration of 3-AT that completely inhibits the growth of the bait was considered for the Y1H assay (20mM for pdistal ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Biochemistry 2022Quote: ... Elutions from the initial IMAC capture step were directly cleaved with His-tagged HRV 3C protease (produced in-house) prior to concentrating using an Amicon 30 kDa MWCO concentrator (Millipore) and dilution with ion exchange chromatography (IEX ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen cell pellets were thawed and then lysed using ice cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 (Sigma), 10 mM NaCl (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl) using PD-10 columns (GE) and subjected to overnight cleavage at 4 °C with His-tagged TEV protease (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Biochemistry 2023Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Microbiology 2023Quote: ... 30 min at 4°C) and purified by immobilized metal ion affinity chromatography (IMAC) using a His-Select Nickel Affinity Gel (Sigma) in a fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: ... pET28a-His-DNTTIP1 has been generated by in frame insertion of DNTTIP1 cDNA into BamH1/Xho1 sites of pET28a(+) (Novagen, kindly provided by Dr ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by four washes with 200 μl of incubation buffer and incubation with 50 μl of anti-His-HRP antibody (Sigma), diluted 1:2000 in incubation buffer for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... A soluble extract was prepared by centrifugation at 14,000 × g for 30 min and applied to Ni-NTA His-bind Resin (Millipore Sigma) equilibrated with binding buffer (50 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant supplemented with final 10 mM imidazole was incubated with His-Select Nickel Affinity Gel (P6611, Lot No. 110M5166, SIGMA) for 1.5 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered lysate was then incubated for 30 min with 1 ml of equilibrated HIS-Select HF Ni-affinity gel (Sigma) and loaded onto a gravity flow column ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 nM campthothecin (Sigma), 250 nM etoposide (Sigma) ...
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bioRxiv - Biophysics 2020Quote: ... 5% FBS (Sigma-Aldrich) and 1X antibiotic antimycotic (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... containing 5% FBS (Sigma). HEK293T Lenti-X were cultured in DMEM (VWR ...
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bioRxiv - Cancer Biology 2021Quote: ... with 5% FBS (Sigma). Cells were tested for mycoplasma every 3-4 months ...
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bioRxiv - Cell Biology 2020Quote: ... Maraviroc (5 μM, Sigma) was added to the culture medium 30 minutes before adding conditioned medium.
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... 5 % CHAPS (Sigma-Aldrich), 5 mM TCEP (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2020Quote: ... 5% glycerol (Sigma-Aldrich), 1% sodium deoxycholate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... 5 QX314 (Sigma-Aldrich), 0.3 EGTA ...
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bioRxiv - Cell Biology 2022Quote: ... + 5% DMSO (Sigma-Aldrich) for >24 hours in a freezing container before transfer to liquid nitrogen ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... 5% BSA (A7906, Sigma) in PBS (10010-23 ...
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bioRxiv - Genomics 2020Quote: ... 5% BSA (A7906, Sigma) in PBS (10010-23 ...
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bioRxiv - Immunology 2021Quote: ... 5 mM HEPES (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-5-HT (Sigma), anti-PKCα (Santa Cruz Biotechnologies) ...
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bioRxiv - Genetics 2020Quote: ... 5’ sgRNAs (Sigma-Aldrich) were cloned into lentiCRISPR v2 (Addgene 52961 ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μM forskolin (Sigma), 2 μg/ml heparin (Sigma)) ...
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bioRxiv - Developmental Biology 2019Quote: ... 5% goat serum (Sigma), 0.05% Tween 20 ...
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bioRxiv - Developmental Biology 2019Quote: ... 5% goat serum (Sigma) and 0.05% Tween 20 (Sigma) ...
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bioRxiv - Immunology 2019Quote: ... 5 mM cysteamine (Sigma), 50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM G6Pi (Sigma) for redox-balanced lysate or ddH20 (oxidising lysate) ...
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bioRxiv - Developmental Biology 2019Quote: ... or 5 % BSA (Sigma) in TBS containing 0.1% Tween at RT for 1 hour and incubated with primary antibodies at 4 °C overnight ...
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bioRxiv - Molecular Biology 2019Quote: 5-fluorouridine (Sigma, F5130) was added to cell culture with final concentration of 2mM ...
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bioRxiv - Immunology 2019Quote: ... + 5% FBS (Sigma-Aldrich) for 90 min at 37°C with gentle shaking ...
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bioRxiv - Neuroscience 2019Quote: ... IGEPAL solution (5%, Sigma) was added to a final concentration of 0.32% ...
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bioRxiv - Molecular Biology 2020Quote: ... 5-azacytidine (Sigma A2385), and chaetocin (Cayman Chemicals 13156) ...
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bioRxiv - Genetics 2020Quote: ... 5% donkey serum (Millipore), 1.5% horse serum (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... 5 mM MgCl2 (Sigma), 0.1 % Triton-X 100 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM MgCl2 (Sigma)] ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 mM ethanolamine (Sigma), 50 nM sodium selenite (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM retinol (Sigma) and 100 mM palmitic acid (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... and 5% donkey (Sigma) or goat (Dako ...