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Citations for Millipore Sigma :
1551 - 1600 of 3780 citations for IGSF11 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells were matched-paired and incubated in CO2-free conditions in L-15 medium (supplied with 10% FCS 2mM L-glutamine and 10μg/ml Pen/Strep) and LPS (Sigma-Aldrich) or CSF-1 (Miltenyi Biotec Australia Pte Ltd) ...
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bioRxiv - Microbiology 2020Quote: ... with 8% fetal calf serum (FCS; Bodinco) supplemented with 100 IU/ml of penicillin and 100 μg/ml of streptomycin (Sigma), and 2 mM L-Glutamine (PAA) ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Developmental Biology 2020Quote: Spinal cords were incubated at 38°C for 20 minutes with F12 medium supplemented with 5% FCS (F7524; Sigma-Aldrich), 20mM HEPES buffer (15630-049 ...
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bioRxiv - Microbiology 2020Quote: ... containing 4.5 g l−1 D-glucose and L-glutamine supplemented with 10 % fetal calf serum (FCS; Sigma Aldrich, USA), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin (PAN Biotech ...
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bioRxiv - Genetics 2022Quote: ... and 10% heat-inactivated fetal calf serum (FCS) and stimulated with 25 μg/ml lipopolysaccharide (LPS) (Salmonella typhosa origin, Sigma). CD8 T cells were stimulated using anti-CD3 (10μg/ml plate-bound ...
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bioRxiv - Microbiology 2022Quote: ... then cells were replenished with DMEM containing 10% (v/v) FCS and 50 μg/ml gentamicin (Sigma Aldrich, cat# G1397) and incubated for 72 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the culture medium was exchanged to medium without growth factors but supplemented with 1% fetal calf serum (FCS, Sigma Aldrich). The cells were differentiated for 5 days followed by fixation or preparation for WB or RNA isolation ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown for 3 days in phenol red-free media supplemented with 10% charcoal-stripped FCS (-E2) and subsequently treated with 100nM E2 (Sigma) for the indicated times.
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bioRxiv - Cancer Biology 2023Quote: ... 4T1 cells expressing shRNA to knock down Beclin 1 or Atg7 expression or control 4T1 cells expressing scrambled shRNA were treated with mouse rPD-1-Fc (1 μg/mL) for 30 minutes and subsequently incubated in medium containing doxorubicin (0.5 μM; Sigma #D1515) for one hour ...
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bioRxiv - Immunology 2023Quote: Cells were cultured in R10 medium, (RPMI 1640 containing 10% heat inactivated FCS, 50μM 2-mercaptoethanol with penicillin and streptomycin, (Sigma-Aldrich) and incubated at 37°C ...
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bioRxiv - Immunology 2023Quote: ... USA) cells and the respective sublines were maintained in RPMI-1640 medium containing 8% fetal calf serum (FCS) (Sigma, F7524), 2 mM L-glutamine ...
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bioRxiv - Immunology 2023Quote: ... 8129) cells were maintained in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS, Sigma cat. F7524) and 100 U/ml of penicillin/streptomycin ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Biochemistry 2023Quote: ... the Siglec-7-Spy Tag-TEV-Fc was concentrated using an AMICON (Molecular Weight Cut Off 30 kDa – Merck Millipore) to approximately 0.15 mg/ml measured via NanoDrop as described above ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated by incubating the pooled tissue in 250gl PBS supplemented with 10% fetal fetal calf serum (FCS) and 1 mg/ml collagenase (Sigma) for 45 minutes at 37° C with shaking at 750 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... Inocula were removed and replaced with DMEM containing 1% FCS with a semi-solid overlay consisting of 1.5% carboxymethyl cellulose (Sigma-Aldrich). Cells were incubated for 4-5 days ...
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bioRxiv - Immunology 2023Quote: ... Pellets were recovered in PBS 2% FCS and erythrocytes were removed using a red blood cell lysis buffer (Sigma-Aldrich) before being filtered onto a 40-µm cell strainer (Dutscher) ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained for 30 min on ice in PBS containing 2% FCS and 0.1% sodium azide (cat. S2002, Sigma-Aldrich). To examine intracellular cytokine production ...
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bioRxiv - Immunology 2024Quote: ... All bacteria cultures were then centrifuged at 3,000 rpm for 10 min and the bacterial pellet was resuspended into 300μl of RPMI/5% FCS with gentamycin (Sigma Aldrich) (30 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HEK cells used for electrophysiological study were adhered to the poly-L-lysine-coated (MW 30,000-70,000, Sigma-Aldrich; 0.1 mg/mL for 2 hr at 37 ºC) coverslips (CS-5R ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
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bioRxiv - Cell Biology 2020Quote: ... fibrinogen (human plasma derived) and WKYMVm were from Sigma Aldrich. Recombinant fNLFNYK ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with human rhinovirus 3C protease (8 units/ml; Novagen), and incubated without agitation (4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Human Dermal Fibroblasts were purchased from Sigma (cat # 106-05A). A vial of cells was thawed ...
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bioRxiv - Immunology 2021Quote: ... and human interferon-a 2B (15 ng/mL, Sigma-Aldrich). All cell counts were done manually using a hemocytometer and trypan blue exclusion.
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bioRxiv - Molecular Biology 2021Quote: ... human IPMK cDNA was subcloned into pGEX4T plasmid (Sigma Aldrich), expressed in Escherichia coli ...
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bioRxiv - Biochemistry 2020Quote: Human serum-derived Factor IX was purchased from Sigma Aldrich, MO ...
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bioRxiv - Microbiology 2022Quote: Human Cerebral Microvascular Endothelial cells hCMEC/D3 (obtained from Millipore) were grown in EndoGRO-MV complete media (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse anti-human nuclei clone 235-1 (1:250; Millipore), rabbit anti-histone H3.3K27M mutant (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant human type I or type III collagen (Millipore Sigma) were immobilized on a CM5 sensor chip (Cytiva ...
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bioRxiv - Genomics 2020Quote: ... human collagen type III (88 ng per well; Sigma-Aldrich) or a combination of human collagen type I and type III (88 ng of each collagen type per well) ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 5% (v/v) Human AB serum (Sigma-Aldrich), 1% (v/v)
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bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Bioengineering 2019Quote: Human Umbilical Vein Endothelial Cells (UVECs) were obtained from Sigma® (200P-05N ...
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bioRxiv - Genetics 2019Quote: ... A rabbit anti-human FAH antibody (Cat. no. HPA041370; Sigma) was added in PBT at 1:200 and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
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bioRxiv - Genomics 2020Quote: ... and/or 0.4μg human GST-tagged PP2A-α (Sigma, SRP5336) in the presence or absence of AZ’5576 or okadaic acid (Abcam ab120375 ...
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bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4 °C.
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bioRxiv - Immunology 2019Quote: ... cells were blocked in 10% human serum (Sigma-Aldrich, H4522) for 10 minutes at 4°C prior to antibody staining.
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bioRxiv - Bioengineering 2019Quote: ... 1 U/ml human thrombin (EMD Millipore, Burlington, MA, USA) and 2 μg/ml ciprofloxacin (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20ng/ml human epidermal growth factor (hEGF) (Sigma, Cat. No.E9644) and allowed to proliferate over one or two passages ...
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bioRxiv - Immunology 2021Quote: ... were coated with either goat anti-human IgG (Sigma #I2136) or IgM (Sigma #I1636 ...