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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature with an antibody against mHTT protein (1-82 aa, Millipore, MAB5492 1:500), and either NeuN (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of extracted protein was transferred to Eppendorf tubes to incubate with anti-FLAG-antibody (1:1000; Millipore Sigma) or anti-HIS-antibody (1:300 ...
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bioRxiv - Genetics 2021Quote: ... glial fibrillary acidic protein (GFAP; polyclonal 1:1000, ZO334, DAKO-Agilent, Santa Clara, CA, USA; monoclonal 1:1000, MAB 3402, Millipore, Burlington ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP ...
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bioRxiv - Neuroscience 2022Quote: ... using rabbit antisera to the microglial protein Iba1 (1:1000; Fujifilm Wako Pure Chemical Corp) and AlexaFluor Donkey anti-rabbit 594 (1:1000; Millipore). Slides were cover slipped with Vectashield containing DAPI (Vector laboratories).
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with primary antibodies against protein of interest (NPR, Santa Cruz, sc-39008, 1:100; GFP, Aveslab, GFP-1020, 1:5,000; FLAG, Sigma, F1804 ...
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bioRxiv - Immunology 2020Quote: ... plasma samples were incubated at a dilution of 1:100 and bound antibodies were detected with goat anti-human IgM/HRP (Sigma: cat. A6907, 1:6’000 dilution), goat anti-human IgG/HRP (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... Micropatterned coverslips were functionalized with a solution of 10 µg mL-1 FN from human plasma (Sigma Aldrich #F1056) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... solutions of 89Zr-labeled TDM1 (4 μCi/μg) were prepared in PBS (pH 7.5) containing 1% w/v human serum albumin (HSA, Sigma) and 0.1% w/v sodium azide (NaN3 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1×105 ESCs were plated on a 3.8 cm2 plate coated with human plasma fibronectin (Millipore, cat. no. FC010) and cultured in N2B27 medium supplemented with 12 ng/ml bFGF (R&D Systems ...
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bioRxiv - Cancer Biology 2019Quote: The AsPC-1 and Capan-2 human PC cell lines were obtained from Sigma-Aldrich (St. Louis, MO, USA) and Thermo Fisher (Waltham ...
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bioRxiv - Neuroscience 2019Quote: ... the eye cup was treated for ∼ 15 minutes with human plasmin (∼ 50 µg mL−1, Sigma or Haematologic Technologies) to aid vitreous removal.
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bioRxiv - Bioengineering 2020Quote: ... Three sections at varying depths within the tumor are immunostained with mouse anti-human nuclei (clone 235-1, Millipore) followed by secondary Dylight 488 horse anti-mouse (Vector) ...
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bioRxiv - Microbiology 2021Quote: ... THP-1 (ATCC® TIB-202™) human monocyte cell line was cultured in RPMI-1640 medium (Sigma, R8758) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed with PBST and incubated with a goat anti-human Fc−peroxidase antibody diluted 1:5,000 (Sigma) in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were then washed and incubated with a 1:5,000 dilution of peroxidase-conjugated anti-human Fc antibody (Sigma) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... 50% Neurobasal Medium, 0.5% N2 supplement, 1% B27 without vitamin A [Life Technologies, 12587-010], 0.025% [v/v] human insulin [Sigma, I9278] ...
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bioRxiv - Cancer Biology 2022Quote: ... human tumor slides were stained by immunohistochemistry with an antibody against Tenascin-C (1:300, EMD Millipore, Cat. ab19011), a strain-sensitive ECM protein known to be present in LUAD [9 ...
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bioRxiv - Bioengineering 2019Quote: ... The antibodies used were mouse anti-human HSA (1:500; clone HSA-11, A6684; Sigma Aldrich; St. Louis, MO) and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40 ...
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bioRxiv - Pathology 2020Quote: ... the suspension adjusted to 1 × 106 parasites/mL in PBS and was incubated with 20% human complement (Sigma Aldrich). After 30 min ...
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bioRxiv - Biochemistry 2019Quote: ... ti-sodium citrate and sodium hydroxide were purchased from Merck Company and Human serum albumin (96%) and ANS (1-anilino-8-naphthalene sulfonic acid) were purchased from Sigma-Aldrich.
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bioRxiv - Neuroscience 2019Quote: After extensive washes in PB and 0.05 M Tris-buffered saline (pH 7.4; TBS) sections were blocked in 1 % human serum albumin (HSA; Sigma-Aldrich) in TBS ...
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bioRxiv - Genetics 2020Quote: ... were co-expressed with the C-terminal domain of human DNMT3L (residues 178–386 of NCBI accession NM_175867) on a modified pRSFDuet-1 vector (Novagen), in which the DNMT3B or DNMT3A sequence was preceded by a hexahistidine (His6 ...
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bioRxiv - Immunology 2022Quote: ... Secondary incubation was performed with 1:20 000 dilution of HRP-conjugated anti-human IgG antibody (Sigma Aldrich SAB701283) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and AP-labeled goat anti-human IgA (α-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) was used for detection ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were extensively washed in 0.1M PB and TBS and blocked in 1% human serum albumin (HSA; Sigma-Aldrich) solution for 1 h ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The probe was resuspended in a solution containing 38 ng/μL human Cot-1 DNA (Millipore Sigma, Cat # 11581074001), 256 ng/μL yeast tRNA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins precipitated using protein-A Agarose (Sigma-Aldrich) prior to analyses by Western blotting.
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bioRxiv - Microbiology 2022Quote: ... proteins proteins were electrophoretically transferred to nitrocellulose membranes (Millipore) using a Transblot semi-dry transfer cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... were transduced when reaching around 50-70% confluence with 1x concentrated virus diluted in ADF+++ and supplemented with hEGF and 5 µg/µl polybrene (Sigma). The cells were incubated for 12-18 h with the viral suspension ...
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bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The parental HEK293T cells were transduced with this virus and the HEK293T-ACE2 cell line was selected and maintained with medium containing puromycin (Sigma). ACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... N2a cells’ media was exchanged with virus-containing supernatant at 48- and 72-hours post-transfection with 8µg/ml polybrene (Sigma #H9268). N2a cells were expanded and FACS sorted for mCherry+ cells.
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bioRxiv - Biochemistry 2020Quote: ... The medium was replaced after 72 h and cells containing the integrated virus were selected with Puromycin (50ng/μl) (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Viruses were titrated by infecting THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 μg/ml, Sigma) and incubating for 48 h ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Molecular Biology 2021Quote: HEK293FT-rKSHV cells were generated by infecting HEK293FT cells with concentrated rKSHV.219 virus (43) in the presence of 8 µg/mL polybrene (Sigma) and by spinoculation (800 g for 30 min at room temperature) ...
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bioRxiv - Microbiology 2019Quote: ... or PMA-treated THP-1 cells (2×105 cells/ml) with dilutions of sucrose purified virus in the presence of polybrene (8 µg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD ...
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bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
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bioRxiv - Cell Biology 2022Quote: ... 50,000 MBS-MEFs were seeded in 6-well plates and exposed to the virus particles mentioned above in serum-free media supplemented with 6 µg/ml polybrene (Sigma). Cells with high or low expression of tdTomato were selected by FACS (BD Biosciences Aria III).
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Cancer Biology 2022Quote: ... The same method was used for virus to be used for GSCs (DMEM was changed to NBM).The shRNA construct number TRCN0000152163 from Sigma human TRC shRNA library was used for knockdown studies of human FMOD ...
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bioRxiv - Microbiology 2020Quote: ... Mock- and virus-infected cells were harvested at 16 h post infection and stored at −80°C in RNALater (Sigma) until RNA extraction ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was then passed through a 0.45 µm filter to remove debris and virus was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Sigma, UFC9100). For this ...
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bioRxiv - Cancer Biology 2019Quote: ... Functional titration was performed by transduction of cells with serially diluted virus in the presence of polybrene (4 μg/ml, Sigma) for 6 hours followed by puromycin (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... media was removed and replaced with a mixture of culture media (50%) and crude virus (50%) containing Polybrene (8 μg/ml, Sigma). Transduction media was removed and replaced with fresh culture media 24 h later ...
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bioRxiv - Neuroscience 2021Quote: ... was used to obtain 5×105 cells single-cells per well of a Matrigel-coated 6-well plate that were incubated with concentrated virus in mTeSR1 with 10 μM ROCK inhibitor and 8 μg/ml polybrene (Sigma) for 8 hours ...