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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Five microliters of a 5 mg mL−1 solution of protein G (Sigma-Aldrich, Tokyo, Japan) was added to the flow chamber (about 5 μL) ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein production was induced by adding 1 mM IPTG and 10 µM all-trans retinal (Sigma) at an absorbance of 0.8–0.9 at 600 nm ...
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bioRxiv - Genetics 2022Quote: ... hPSC-CMs were stained with sarcomreirc protein TNNT2 (Catalog #: HPA017888 Lot #: A91786, Sigma, dilution 1/200), secondary antibody Goat anti-Rabbit IgG Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
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bioRxiv - Physiology 2019Quote: ... was from BD Biosciences; citrate synthase (CS; SAB2701077) and mitochondrial fission protein 1 (FIS1; HPA017430) were from Sigma Aldrich; vitamin D receptor (D-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM of isopropyl-β-D-thiogalactoside (IPTG) (Sigma Aldrich). Expression was continued in a bacterial shaker at 30°C and 150 rpm for 6h ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Cell Biology 2020Quote: ... the culture medium was supplemented with 1 μM Rho-associated protein kinase (ROCK) inhibitor (Y27632, Millipore). Recombination was induced with 100 nM 4-hydroxytamoxifen (4OHT ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μg of proteins were mixed with 0.004% Bromophenol blue and 1% β-Mercaptoethanol (Sigma-Aldrich), incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 140μg of mitochondrial protein was incubated with 1:100 of 1M dithiothreitol (Sigma-Aldrich, SKU 43815), followed by 1:50 dark incubation in 1M iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2023Quote: ... with the protein aqueous solution (1 mg/ml of PLL, Sigma-Aldrich, in PBS pH 10.4), at a 1:2 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and bands were normalized to total protein and the housekeeping gene Gapdh (1:10,000, Millipore, NP_002037).).
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 10k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 1% FBS (v/v) and protein expression enhancing sodium butyrate (65) solution (SIGMA-ALDRICH) to a final concentration of 2 nM were added to the cells to make up to the final volume of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was determined using the bradford protein assay (Sigma-Aldrich). Proteins were separated in 8% SDS-polyacryamide gel (PAGE-SDS ...
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bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: Protein concentrations were determined using the BCA protein assay (Sigma-Aldrich) using bovine serum albumin as a standard ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was measured by BCA Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Thirty microliters of protein G/protein A agarose beads (EMD Millipore) were washed with lysis buffer (containing protein inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... The protein concentration was determined by a BCA protein assay (Sigma). A total of 50 µg protein extract was mixed with 5× Laemmli loading buffer (50% glycerol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 50 μg were resolved on an SDS/PAGE denaturing gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined using the BCA protein determination reagent (Sigma), and 60 μg were resolved on an SDS/PAGE gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein quantification was performed with the BCA Protein Assay Kit (Millipore); for each sample ...
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bioRxiv - Microbiology 2024Quote: ... then removed the proteins with a 10K MWCO protein concentrator (Millipore) and quantified the resulting HOSCN solution (typically 3.5 to 4 mM ...
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bioRxiv - Biochemistry 2020Quote: LX-2 human hepatic stellate cells [47] (Millipore) were cultured in DMEM supplemented with 10% fetal bovine serum at 37°C under 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10 μg/ml recombinant human insulin (Sigma). BT474 cells were cultured in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: F4883-500MG) were purchased from Sigma Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... human plasma-derived (Sigma, Cat. No #: T6884-100UN) and Fibronogen ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg/ml of recombinant human insulin (Sigma), 2 IU/ml heparin (Affymetrix) ...
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bioRxiv - Cell Biology 2022Quote: ... Human cells were pretreated with glycine (Millipore, 104201) at a concentration of 50 mM for at least 10 minutes before stimuli was added.