Labshake search
Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... fibrinogen (FG) from human plasma (30 mg/mL, Sigma-Aldrich), thrombin from human plasma (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: Human IGF2BP2 was cloned into p3XFLAG-myc-CMV 26 (Sigma) and pLVX-E1Fα-IRES-mCherry-Puro (Takara Bio ...
-
bioRxiv - Cell Biology 2024Quote: ... The single cells were incubated with human IgG (Sigma-Aldrich) at 4°C for 15 min to block nonspecific binding sites ...
-
bioRxiv - Developmental Biology 2023Quote: ... injection of human chorionic gonadotropin (5 IU, #CG5; Sigma-Aldrich) 48 h later ...
-
bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
-
bioRxiv - Cell Biology 2023Quote: The following primary and secondary antibodies were used at indicated concentrations: anti-human α-actinin1 (mouse monoclonal, BM75.2, Sigma Aldrich, A5044; WB 1:1000), anti-human talin (mouse monoclonal ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
-
bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
-
bioRxiv - Microbiology 2024Quote: ... using 100 mg of human stool sample per 1 ml of PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma-Aldrich, St-Louis, MO, USA). The suspension was incubated for 10 min to allow large particles to settle ...
-
bioRxiv - Biochemistry 2019Quote: Insulin and amylin concentration in plasma were determinate by ELISA (Insulin, Cat # EZRMI-13K and Amylin, Cat # EZHA-52K, Merck Millipore) following manufacturer manual ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Insulin concentration of the supernatants was measured with a mouse/rat insulin ELISA (EZRMI-13K, EMD Millipore, Billerica, MA, USA).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plates were then rewashed three times with PBST before the addition of 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, UK) and incubated for 12 minutes in the dark at RT ...
-
bioRxiv - Immunology 2019Quote: ... The peritoneal lavage fluids were collected and concentrated for ELISA analysis with Amicon Ultra 10 K filter (UFC900308) from Millipore. Peritoneal exudate cells were collected and analyzed by FACS.
-
bioRxiv - Microbiology 2021Quote: ... and stored at −80°C until particle concentration assessment by p24 ELISA (Innogenetics) while the rest of the supernatant was concentrated by filtration on Centricon units (Centricon Plus-70/100K, Millipore) and frozen at −80°C for RNA extraction ...
-
Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsybioRxiv - Neuroscience 2023Quote: ... Lysates were removed and protein antibody-conjugates linked to the bottom of the plate were washed three times with 300 µL of ELISA wash buffer (100mM Tris buffered saline, pH8.2, 2mM MgCl2, 0.5% Tween-20 – all Sigma-Aldrich, Ireland). Protein was eluted by incubating wells in 2 X Laemelli buffer for 10 mins ...
-
bioRxiv - Immunology 2022Quote: ... antibody levels were detected using a secondary anti-murine IgG conjugated with alkaline phosphatase and the substrate for alkaline phosphatase yellow (pNPP) liquid substrate system for ELISA (cat. P7998, Sigma). After 30 minutes of incubation ...
-
bioRxiv - Cell Biology 2021Quote: VCAM-1 in the media (1:100) from MCECs stimulated with MG was measured using commercial kits from Sigma, according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
-
bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
-
bioRxiv - Bioengineering 2020Quote: ... MSCs embedded in the PEG-NB gels were stained for α-SMA with FITC-conjugated mouse monoclonal anti-human α-SMA (F3777, Sigma; dilution 1:300), cellular extra domain-A (ED-A ...
-
bioRxiv - Immunology 2021Quote: ... 50 μl/well of 3,3’-5,5’-tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (T040, Sigma-Aldrich Co. LLC., St. Louis, MO) was added to the plate ...
-
bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
-
bioRxiv - Immunology 2020Quote: ... Absolute OVA-specific IgG1 concentration was determined for each sample by ELISA as described above using OVA-14 (Sigma, MO USA) as a standard and equalized across all samples ...
-
bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
-
bioRxiv - Immunology 2019Quote: ... The resulting hybridomas were screened by ELISA for reactivity with STm whole cells and purified LPS (Sigma-Aldrich, St. Louis, MO).
-
bioRxiv - Microbiology 2020Quote: ... The ELISA plates were washed 4 times by PBST and then 100 µL of 2000-fold diluted anti-strep-HRP (Millipore Sigma) were added into each well for another 1 hr at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...
-
bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
-
bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
-
bioRxiv - Molecular Biology 2023Quote: ... Plasma was collected from blood during the GTT to determine insulin levels by ELISA (EZRMI-13K, EMD Millipore Corporation, Burlington, US). Mice were sacrificed at day 7 after a 3h fast ...
-
bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype control were diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
-
bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype were control diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
-
bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
-
bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
-
bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
-
bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
-
bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
-
bioRxiv - Neuroscience 2020Quote: ... 25μg/ml human insulin (Sigma-Aldrich, MO, US; Cat. No. #I9278) and 100 nM RA (Sigma-Aldrich ...
-
bioRxiv - Genomics 2020Quote: ... The Milliplex Map Human TH17 Magnetic Bead Panel (Millipore, Burlington, MA) was used to measure IL-33 levels in thawed supernatants at the University of Chicago Immunology Core facility using standard protocols ...
-
bioRxiv - Biophysics 2019Quote: ... or fibrinogen (0.02 mg/mL, from human plasma, Sigma # F-3879) overnight at 4 °C unless otherwise stated.
-
bioRxiv - Systems Biology 2019Quote: ... with standard curves of purified human RBP4 (72) or TTR (Sigma) protein on each blot (57) ...
-
bioRxiv - Biochemistry 2019Quote: ... Human serum albumin (catalog # A3782) was from Sigma-Aldrich (Taufkirchen, Germany), fetal bovine serum was from Gibco ...
-
bioRxiv - Microbiology 2019Quote: ... filter’s membranes were coated with type IV human placenta collagen (Sigma) for 24 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...
-
bioRxiv - Cell Biology 2019Quote: ... Other media components tested were: Human Long R3 IGF1 (Sigma, 91590C), thiazovivin (LC Labs ...
-
bioRxiv - Cancer Biology 2019Quote: ... resuspended in culture medium containing 100ng/ml human IFNγ (Sigma-Aldrich), dispensed into 384-well plates (BD Falcon ...