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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and biotinylated-Hyaluronan Binding Protein (Millipore, 1:50) at 4°C ...
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bioRxiv - Systems Biology 2024Quote: ... The proteins were reduced with TCEP (Sigma-Aldrich), final concentration 5 mM for 30 min at 37 °C and alkylated with 10 mM IAA (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Parallel protein A/G beads (EMD Millipore, #IP0515ML), H3K4Me (Cell Signalling Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to nitrocellulose (Sigma #10600001) using a wet transfer apparatus (Bio-rad #1704070) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1% protein inhibitor cocktail (100×) (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... and proteins alkylated with 55 mM iodoacetamide (Sigma) in 100 mM NH4HCO3 for 20 mins at RT (protected from light) ...
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bioRxiv - Cancer Biology 2024Quote: ... 50μg protein emulsified with complete Freund’s adjuvant (Sigma) was subcutaneously (s.c. ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using BCA1 from Sigma. Thirty micrograms of protein lysate were separated by 6% Bis-Tris Gel ...
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bioRxiv - Neuroscience 2024Quote: ... 200 µL of protein-A Sepharose beads (Sigma) was added to the 50 mL of supernatant in the presence of anti-protease (Sigma ...
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bioRxiv - Microbiology 2024Quote: Total protein was extracted by RIPA (Sigma-Aldrich) buffer supplemented with protease and phosphatase inhibitors and protein concentration was determined by a BCA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and lysed with BugBuster Protein Extraction Reagent (Millipore). The protein was batch bound to Ni-NTA resin ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was then transferred to PVDF membranes (Millipore) and blocked (BioRad Everyblot 12010020 ...
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Integration of distinct cortical inputs to primary and higher order inhibitory cells of the thalamusbioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Green Fluorescent Protein (Millipore/MAB3580, Monoclonal), Chicken Anti-Green Fluorescent Protein (MilliporeSigma/AB16901 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to a nitrocellulose membrane (Millipore) by semidry blotting and stained with the indicated antisera in PBS/ Intercept® (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... anti-myelin basic protein (MBP) (EMD Millipore, MAB386), chicken IgY anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were detected using ECL reagent (Millipore) and the Amersham Imager 680 (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore) using a semi-dry transfer system at a constant current of 400mA for 30 minutes in transfer buffer (Tris 22.5 mM ...
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bioRxiv - Plant Biology 2024Quote: ... and Protein A agarose/salmon sperm DNA (Millipore) were used for immunoprecipitation ...
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bioRxiv - Neuroscience 2024Quote: Proteins were reduced (10 mM DTT, EMD Millipore) and alkylated (30 mM iodoacetamide ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.1% protein inhibitor cocktail (Sigma- Aldrich: P8849)] ...
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bioRxiv - Immunology 2024Quote: ... Transfer the separated proteins onto PVDF membranes (Millipore) for immunoblotting.Immunoblot the PVDF membranes (IPVH00010 ...
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bioRxiv - Genomics 2024Quote: ... and EZview Red Protein G beads (Sigma, E3403) were incubated with RNA for 3 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was expressed in BL21-Rosetta(DE3) (Novagen) E ...
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bioRxiv - Immunology 2024Quote: ... Protein was transferred to a PVDF membrane (Millipore) using a Trans-Blot Turbo transfer system (BioRad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 mg/ml lysozyme (Sigma-Aldrich, protein ≥90%), 0.5 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Cancer Biology 2024Quote: ... Magna ChIP protein G magnetic beads (Sigma Aldrich) were blocked overnight with 0.05% BSA (Roche) ...
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bioRxiv - Cancer Biology 2024Quote: ... 15 µl of Protein G magnetic beads (Millipore) is then added to the Protein-Antibody complex and incubated at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were subsequently transferred to PVDF membrane (Millipore). Blocking was performed at room temperature for 1 hour with 2.5% BSA ...
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GRASP55 Safeguards Proper Lysosome Function by Controlling Sorting of Lysosomal Enzymes at the GolgibioRxiv - Cell Biology 2024Quote: ... proteins were transferred to PVDF membranes (IPVH00010, Millipore), except for M6P and phospho-TFEBS211 blots for which nitrocellulose membranes (#10600002 ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred to PVDF (Sigma) membranes at 40 V for 2 h ...
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Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: ... The protein was removed by Amicon concentrators (Millipore), and the concentrate was washed two times with three volumes of water ...
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Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: ... The protein was removed by Amicon concentrators (Millipore), and the concentrate was washed two times with three volumes of water ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with MS-approved Trypsin (Sigma). Protease digestion was stopped by addition of trifluoroacetic acid to 0.5% ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was mixed with 6x LSB (Sigma-Aldrich) and 4% DTT (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The horseradish peroxidase (HRP) secondary antibodies polyclonal antibody against FLAG and polyclonal antibody against HA were from Sigma-Aldrich. All secondary antibodies conjugated with Alexa Fluor dyes for immunostaining were from Life Technologies.
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bioRxiv - Immunology 2021Quote: ... Liver triglyceride and total cholesterol concentrations were measured using commercial kits (all from Instruchemie) and expressed as nanomoles per milligram of total protein content using the Bradford protein assay kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: CDK5 was immunoprecipitated from cells samples at different circadian times (CTs) (circa 200 μg of protein extracts. After immunoprecipitation at 4°C overnight with 2x Protein A agarose (Sigma-Aldrich), samples were diluted in washing buffer and split in two halves ...
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: Protein adsorption to the films was analyzed by incubation in a bovine serum albumin-FITC protein solution (1mg/ml, Sigma Aldrich) overnight at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... pLysS cells expressing His-topoIB and T18 tagged genome segregation proteins in different combinations using the kit Protein G immunoprecipitation from Sigma-Aldrich as described previously (Maurya et ...
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Systems Biology 2021Quote: ... the gel was scanned for total protein quantification and the proteins were subsequently transferred to an Immobilon-FL PVDF membrane (CAT # IPF00010, EMD Millipore). The membrane was probed with Rabbit anti-RAN (CAT # PA 1-5783 ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: Native PAGE-separated proteins or SDS PAGE-separated proteins were then transferred to PVDF membranes (EMD Millipore, catalog #IPVH00010, lot #R1EB02212) and subjected to Western blotting analysis as described previously (Li et al ...
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bioRxiv - Plant Biology 2023Quote: ... then proteins were extracted by adding 5 volumes of protein extraction buffer containing 1X MS-SAFE protease and phosphatase inhibitor (Sigma MSSAFE) and 1mM PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... The ATP level for each sample was normalized to the protein content of each sample using the BCA protein assay kit (Sigma-Aldrich). Results are expressed as fold change ± S.E.M ...
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...