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Citations for Millipore Sigma :
1551 - 1600 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore; Cat # IPFL00010), and nonspecific protein binding was blocked with 3% BSA in Tris-buffered saline for at least one hour at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Tissue proteins were isolated by homogenization in RIPA buffer (Sigma-Aldrich) supplemented with cOmplete Mini protease inhibitor cocktail tablets (Roche Diagnostics ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were concentrated using Amicon Ultra filters (Millipore, Ireland), and confirmed by SDS-PAGE.
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bioRxiv - Biochemistry 2019Quote: ... proteins were transferred to 0.45 µm Immobilon-P PVDF membranes (Millipore) pre activated in methanol ...
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were probed with anti-tubulin (Sigma, 1:10,000 dilution) and anti-GFP (Takara Bio ...
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bioRxiv - Neuroscience 2019Quote: ... protease inhibitors and protein phosphatase inhibitors I and II (Sigma-Aldrich). After an incubation of 30 min on ice and centrifugation at 10 000g for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... All proteins were expressed in BL21 Rosetta DE3 pLysS cells (Novagen) and purified using IMAC as described in detail previously41.
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bioRxiv - Cell Biology 2019Quote: ... proteins transferred to PVDF membranes (Immobilon-FL or Immobilon-P, Millipore) were detected by fluorophore- or HRP-conjugated to secondary antibodies (Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2019Quote: ... and 80 ug/mL myelin basic protein (MBP, Sigma-Aldrich #M1891) in the presence of 25 uM ATP (Sigma-Aldrich A7699) ...
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bioRxiv - Cell Biology 2019Quote: Proteins/peptides were labelled with atto-488 NHS ester (Sigma, 41698). Free dye was separated from labelled protein using G25 desalting columns ...
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bioRxiv - Systems Biology 2019Quote: ... Secondary incubation with anti-Protein G HRP conjugated (Millipore 18-161) at 1:10,000 was performed at RT during one hour ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The total protein extracted was quantitated using Bradford Reagent (Sigma, USA). Bovine Serum Albumin (BSA ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were electro-transferred to PVDF membranes (Merck Millipore, MA, USA). Immuno-detection was performed with polyclonal antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... mouse anti-glial fibrillary acidic protein (GFAP)–Cy3 (Sigma, 1:2000) to show astrocyte structure ...
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bioRxiv - Genetics 2020Quote: ... Proteins were immunoprecipitated using ANTI-FLAG® M2 Affinity Gel (Sigma) for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins expression was detected using an enhanced chemiluminescence kit (EMD Millipore).
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bioRxiv - Immunology 2021Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Systems Biology 2021Quote: ... Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and image acquisition using a ChemiDoc Imaging system (Bio-rad) ...
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bioRxiv - Cell Biology 2020Quote: ... EZview™ Red Protein G Affinity Gel was from Sigma Aldrich. Click-iT™ EdU Cell Proliferation Kit for Imaging ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using Amicon Ultra Centrifugal Filters (Merk Millipore). Selenomethionine derivated PrgL32-208 was purified following the same protocol with the exception that 0.5 mM tris(2-carboxyethyl)-phosphine (TCEP ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blotted with anti-Protein A (1:10,000, Sigma), anti-HA (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... before adding 15 µL of Protein A-Sepharose beads (Sigma-Aldrich) and allowing the incubation to continue for further16 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... the purified protein was concentrated using Amicon Ultra 50 k (Millipore).
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bioRxiv - Biochemistry 2021Quote: ... or protein A/G agarose beads (EMD Millipore, cat. no. IP05) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... the N protein was labelled with ATTO 488 dye (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Heterologous protein expression was achieved using pET28b(+) (EMD Millipore, Burlington, MA), in which open reading frames for the enzymes were cloned into BamHI/XhoI doubly digested vector ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins from peak fractions were concentrated using centrifugal ultrafiltration devices (Millipore) to final concentrations of ∼1 mg/ml (RBD-8h proteins) ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated by ultracentrifugation (Amicon Ultra-15, Millipore) to ~15-20 mg/ml and stored in 5 mM bis-tris propane–HCl (BTP-HCl) ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted protein was concentrated using 100-kDa cut off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore, Massachussets, United States).
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Alzheimer Precursor Protein (APP) 22C11 (1:100, MAB348, Millipore), and mouse anti-β-Tub III/Tuj 1 (1:4000 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reconstituted in sterile DMSO (Millipore-Sigma, Burlington, MA, USA) to stock concentrations of 10 mM ...
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bioRxiv - Neuroscience 2020Quote: ... Bound DNA and proteins were eluted with 16 mM biotin (Sigma) in water (at pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... Crosslinked protein was concentrated using 100-kDa cut-off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein was transferred to a methanol-activated PVDF membrane (Millipore) at 25 V for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... total protein was detected by staining with Ponceau S (Sigma-Aldrich). Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA in Tris-Buffered-Saline-0.1% Tween-20 (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore). Densitometry was carried out by FIJI.
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bioRxiv - Cell Biology 2021Quote: ... Protein quantification was done using the Bradford colorimetric assay (Sigma B6916). 20µg of proteins were separated run on a 10% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2020Quote: ... at 1 ug/ml or control protein Bovine Serum Albumin (Sigma) at 1 ug/ml in PBS were coated onto flat bottom ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Resultant protein concentrations were determined using a Bradford assay (Sigma-Aldrich) and purity of the protein preparation determined by SDS-PAGE followed by silver staining ...
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bioRxiv - Pathology 2021Quote: ... Proteins from the resulting gels were transferred to PVDF membranes (Millipore), and analyzed by western blotting with relevant antibodies ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Plant Biology 2020Quote: ... Purified protein was concentrated using an Amicon Filter 3K (#UFC500324, Millipore). In order to avoid any interference with downstream procedures ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were detected using Immobilon Western Chemiluminescent HRP substrate (Millipore MA) as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined using the Bradford Reagent (Sigma, Cat# B6916), SDS-PAGE densitometry ...
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bioRxiv - Biochemistry 2020Quote: ... and the protein inhibitor mixture was from Sigma (catalog no. S8830). Leupeptin ...
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bioRxiv - Biochemistry 2020Quote: ... The protein bands were transferred to PVDF membranes (Millipore, Cat# PVH00010). After being blocked with 5% non-fat dry milk ...