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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 5-Fluoro- 2’-deoxycytidine and 6-thio-2’-deoxyguanosine were obtained from Sigma-Aldrich (Saint- Louis, MO, USA). Phosphodiesterase I was purchased from Affymetrix (Cleveland ...
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bioRxiv - Cancer Biology 2024Quote: ... we used 2’,7’-Bis (2-carboxyethyl)-5 (6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) (Sigma-Aldrich, Darmstadt, Germany). Cells were starved overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... larval sections were counterstained with DAPI (4′,6diamidino-2-phenylindole, Sigma-Aldrich; 1:1000 μL PBS dilution) and incubated for 15 min in the dark at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... they were supplemented once per week with 1/4 strength Hoagland No.2 media (Sigma Aldrich H2395). For tissue harvesting ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µL/g) of the psychedelic drug 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) (Sigma Aldrich) was delivered and HTR recorded and quantified ...
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bioRxiv - Microbiology 2022Quote: ... was previously isolated on the International Streptomyces Project 2 medium (ISP2, dextrose 4 g.L-1 [Sigma-Aldrich, St ...
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bioRxiv - Physiology 2024Quote: ... with 1 µg/mL 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride protease inhibitor (AEBSF; SBR00015, Sigma-Aldrich, Inc.;). Total cell lysate protein concentrations were determined with the Better Bradford Kit (23236 ...
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bioRxiv - Neuroscience 2024Quote: ... N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4) (C8417, Sigma-Aldrich, USA) is a selective neurotoxin targeting the noradrenergic system in the locus coeruleus of the brain ...
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bioRxiv - Neuroscience 2023Quote: ... then in the anti-dopamine receptor antibody diluted in the blocking solution for 5-6 days at 4°C (rabbit anti-dopamine D1A receptor, 1:100, Sigma-Aldrich AB1765P). Sections were washed in PBS and incubated for 2 h at RT in the secondary antibody (goat anti-rabbit Alexa Fluor 555) ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Neuroscience 2020Quote: ... or (4) 2 mg/mL FITC (Millipore Sigma #1.24546) (Figure S2A) ...
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bioRxiv - Neuroscience 2020Quote: ... 2 µM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone FCCP (Sigma), and 0.5 µM of rotenone (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 5,6,7,8-tetradeutero-4-hydroxy-2- heptylquinoline (HHQ-d4, Sigma) was used as an internal standard ...
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bioRxiv - Plant Biology 2024Quote: ... 2-cis 4-trans-abscisic acid (Sigma, Cat # 862169) at 10µM was prepared in ethanol and sterile filtrated with 0.2 µm filter ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 107 cells were fixed in 4% formaldehyde (Sigma) for 10 minutes at room temperature then quenched with glycine ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 mM tris (2-carboxyethyl) phosphine (TCEP) (Sigma-Aldrich)) with 4% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... 2 × 10−4 M L-cystine (C7602, Sigma-Aldrich), 100 μg/mL sodium pyruvate (11360070 ...
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bioRxiv - Biochemistry 2024Quote: ... a 4X 4-(2-pyridilazo)resorcinol (PAR, Sigma-Aldrich) working solution (400 μM ...
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bioRxiv - Microbiology 2024Quote: ... at 4 °C in a 6–30% OptiPrep (Sigma-Aldrich, MilliporeSigma, Burlington, MA, USA) density gradient ...
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bioRxiv - Physiology 2021Quote: ... The resulting CX3CR1CreERT2(+/−)::Ptger4fl/fl offspring were injected at 6 weeks of age with 2 doses of tamoxifen (T5648, Sigma, 4 mg s.c., prepared in corn oil) 48 hours apart ...
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bioRxiv - Biophysics 2023Quote: ... The final master mold received a hydrophobic coating through vapor deposition of 1H,1H,2H,2H-perfluorooctyl-trichlorosilane (Sigma-Aldrich 448931, CAS 78560-45-9) in a vacuum desiccator for 1 day ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 4-Nitroquinoline 1-oxide (4-NQO) (Sigma–Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 4-nitroquinoline 1-oxide (4-NQO) (Sigma-Aldrich #N8141) was added to a concentration of 1 μg/mL or 5 μg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Sprtn KO was induced by treating 4×105 cells with methanol (MeOH) (vehicle control) or 2 μM (Z)-4-hydroxytamoxifen (4-OHT) (Sigma) for 48 h ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fabricated with a final concentration of 1×105 AFC/mL (P3-5) and incubated in EGM-2 +/- 1 mg/mL of the plasmin inhibitor 6-aminocaproic acid (Sigma, A2504) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
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bioRxiv - Cancer Biology 2022Quote: ... cellular suspensions were obtained from cultures and 1 x 106 million tumour cells were resuspended in 6 μL of 2% PVP (Polivinilpirrolidin, Sigma-Aldrich). Microinjection needles were crafted from borosilicate capillaries (1mm outer diameter x 0,58 mm inner diameter ...
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bioRxiv - Cancer Biology 2024Quote: ... were washed two times with PBS and lysed by scraping into 500 μl per plate of lysis buffer (40 mM Tris/HCl pH 7.6, 8 M urea, EDTA-free protease inhibitor complete mini [Roche], and phosphatase inhibitor cocktails 1, 2, and 3 [Sigma-Aldrich] at 1× final concentration according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The stained cells were mounted on a coverslip with a mounting medium containing 1 µg/mL 4ʹ,6-diamidino-2-phenylindole (DAPI) dilactate (Sigma, D9564) and 100 µg/mL p-phenylenediamine (PPD ...
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bioRxiv - Cancer Biology 2024Quote: ... Heat-induced antigen retrieval was performed in a hot water bath in pH 9 Tris/EDTA buffer or pH 6 citrate buffer and stained with primary antibodies anti-beta-2-microglobulin antibody (1:1000, HPA006361 Sigma-Aldrich), anti-beta-2-microglobulin antibody (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.05% Triton X-100 in PBS) for 1h at room temperature and incubated with anti-NeuN (Millipore #MAB377, RRID:AB_2298772, 1:500-1:1000) and anti-NeuroD (Santa Cruz Biotechnology #sc-1084 ...
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bioRxiv - Biochemistry 2024Quote: ... Wells were blocked with 200µL 1% BSA prepared in 1X TBS for 1h and then incubated with anti-FLAG M2-HRP (1:10,000) (Sigma-Aldrich, Cat. no: A8592) for another 1h ...
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bioRxiv - Neuroscience 2021Quote: ... 30 μm coronal sections containing the mHb and the IPN were blocked and permeabilized in 5% NDS and 0.3% TX-100 in PBS for 1h and incubated with rabbit anti GluA1 (1:400, AB1504, Millipore) and mouse anti GluA2 (1:400 ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... cleaved viral particles bearing GPCL were first generated by incubation with thermolysin (THL, 1 mg/ml, pH 7.5, 37°C for 1h; Sigma) or recombinant human cathepsin L (CatL ...
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bioRxiv - Neuroscience 2022Quote: Brain sections were first incubated for 1h at room temperature (RT) in a blocking solution containing 1% BSA (Sigma), 2% Donkey Serum (Biowest ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were then incubated 1h at RT with the rabbit polyclonal antiTWIST1 (1/50, ABD29, Millipore, Burlington; MA, USA); or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200 ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with the following primary antibodies for 1h: mouse anti-α-Tubulin (1:1000, T5168, Sigma-Aldrich) and rabbit anti-Lamin A (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... sample could be incubated also for 1h with oligo-dT-dig probe (1:1000, polyT-dig Sigma HA09131354-004). Antibodies were diluted 1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with 5% normal donkey serum for 1h at room temperature and incubated with Nissl (1:2000, in 0.1M PB, Sigma) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed samples were washed rapidly twice with PBS and 3 times for 15 min with PBS-0.3% Triton X-100 (PBT) before being pre-incubated for 1h in PBT-1% bovine serum Albumin (BSA, Sigma). Samples were incubated overnight at 4°C with primary antibody diluted in PBT-1% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... (3-[1-[3-(Dimethylamino)-propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione (GӦ6983, 100 nM) or with the vehicle dimethyl sulfoxide (DMSO, 1:1000 dilution, Sigma-Aldrich), as control ...
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bioRxiv - Neuroscience 2022Quote: ... mice were injected with the peripheral muscarinic antagonist scopolamine methyl nitrate (1 mg/kg s.c.; #S2250, Sigma Aldrich) to reduce the peripheral effects of pilocarpine ...
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bioRxiv - Biochemistry 2024Quote: ... the medium was changed to DMEM containing 10% FBS and 1 mM Methyl-o- nitropiperonyllysine (mNPK, Sigma-Aldrich) for #2-#4 ...
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bioRxiv - Molecular Biology 2024Quote: Methyl Cellulose Assay: A 10 cm-diameter watch glass was filled with 1% (w/v) methylcellulose (Sigma, Denmark) up to a 4cm radius and 10ml total volume ...
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bioRxiv - Microbiology 2024Quote: ... recombinase expression was induced with 1 mM 3-methyl-benzoate (Sigma Aldrich T36609; m-toluic acid; “3-MB”), and cultures were allowed to resume growth overnight before being used in the tolerization experiment (as described earlier) ...
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bioRxiv - Cell Biology 2020Quote: ... acetylated tubulin (mouse, 6-11B-1, Sigma-Aldrich), ARL6 (rabbit ...