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Citations for Millipore Sigma :
1501 - 1550 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the reaction mixture (in duplicate) was quenched into a MultiScreenHTS Durapore 96-well filter plate (MSHVN4B50; Millipore Sigma) containing 200 µl of 10% charcoal in 0.5% HCl and 50 µl of wash buffer (1 M HCl with 200 mM NaPPi) ...
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bioRxiv - Neuroscience 2020Quote: ... mixed together in a ratio of 10,000 GABAergic neurons:10,000 Glutamatergic neurons:5000 astrocytes directly out of thaw onto 96 well imaging plates coated with Poly-L-ornithine (Sigma-Aldrich) at 2 μg/cm2 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... samples were centrifuged at 10,000g for 5 min and the Acetyl-CoA measurement was performed in a 96-well plate using the Acetyl-Coenzyme A Assay Kit (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: All image-based experiments were conducted on L1 animals which were collected from mixed-stage plates and isolated using a 96 well 11 µM Multiscreen Nylon Mesh filter plate (Millipore: S5EJ008M04) as described previously (Spensley et al. ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 3000 BaF3 cells with the indicated mutation were seeded into a 96 well plate in 150ul of RPMI 1640 (Sigma Aldrich) +10%FBS (Fisher)+1%Penn/Strep (Life technologies) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a non-adherent surface was obtained by covering the surface of a U-bottomed 96-well plate with poly(2-hydroxyethyl methacrylate) (PolyHEMA; #P3932, Sigma-Aldrich) dissolved in ethanol (5 mg/mL) ...
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bioRxiv - Immunology 2020Quote: ... 1×105 neutrophils were seeded into a white 96-well plate (Costar 3917) in the presence of the luminol reagent (1 mmol/L; Sigma-Aldrich). Conidia (MOI 1:2 ...
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bioRxiv - Microbiology 2019Quote: ... Glycerol content for all supernatant samples was measured simultaneously in a single 96-well plate using a Glycerol Assay Kit (Sigma-Aldrich) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The two freshly isolated lenses from each animal were placed into a single 96-well plate containing 200 μL Medium 199 (Sigma-Aldrich) maintained at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of cell supernatant was transferred to a 96-well plate and incubated with an equal volume of sulphanilamide (Sigma, S9251) and N-alpha-naphthyl-ethylenediamine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Samples or kinetics buffers (PBS, PBST: PBS containing 0.02% Tween-20) were dispensed into 96-well black microtiter plates (Millipore, Billerica, MA) with 200 μL in each well ...
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bioRxiv - Cancer Biology 2020Quote: ... 160 µL of the filtrate is transferred to 96- well Griener UV Star® analysis plates (Sigma–Aldrich, St. Louis, MO) containing 40 µL of acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Immunology 2020Quote: ... 50 µl of beads and 50 µl of diluted plasma was added to 96-well microtiter plates (MSBVS 1210, Millipore, USA) pre-wetted with ABE ...
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bioRxiv - Molecular Biology 2021Quote: ... The MIC was determined using the broth dilution method on 96-well plate in cation-adjusted Mueller-Hinton broth 2 (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... were transfected into mouse hypothalamus Pomc neuronal cells (mHypoA-POMC/GFP-1 from Sanbio #CLU500) or human astrocytes (CCF-STTG, ATCC) grown in 96-well plates of 132 using X-tremeGene (Sigma, 6366244001) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... First larval-stage (L1) worms were isolated from mixed-stage plates using 96-well 11 μM nylon mesh filter plates (Millipore Multiscreen) and diluted in M9 to a concentration of ~6 worms/μL ...
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Immunology 2022Quote: ... 5×105 reporter-expressing Jurkat cells were plated in a 96-well plate and cultured for 24 hours with complete RPMI supplemented with the indicated (10ng PMA (Sigma-Aldrich) / 200ng ionomycin (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... Equal numbers of cells were lysed in 96-well plates using 100 μl lysis buffer (1% Triton X-100 (Sigma Aldrich), 25 mM Tricine (VWR) ...
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bioRxiv - Molecular Biology 2023Quote: ... L1 worms were collected by washing mixed-stage plates with M9 buffer and isolated using a 96 well 11 µM Multiscreen Nylon Mesh filter plate (Millipore: S5EJ008M04) to a final concentration of ∼120 animals per well ...
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bioRxiv - Molecular Biology 2022Quote: ... were grown in TYG medium (with/without kanamycin; 8 µg/ml) in 96 well microtiter plates after washing with PBS (Nunclon; Sigma-Aldrich). Optical density at 600nm was measured to examine the growth in replicates at 32 °C for 18 h using Synergy H1 Hybrid multi-mode microplate reader ...
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bioRxiv - Microbiology 2022Quote: ... Aerobic assays were incubated in a 28°C shaker at 225 rpm in 96-well plates sealed with Breathe-Easy membrane (Sigma-Aldrich). All other O2 conditions were generated in a gas-controlled chamber (Ruskinn Invivo2400 ...
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bioRxiv - Microbiology 2023Quote: ... and/or eGFP fluorescence (Ex/Em: 488 nm / 510 nm) was measured in clear 96 well plates sealed with a Breathe-Easy® membrane (Sigma) with linear shaking at 37°C for 48 h using a Spark 10M plate reader (Tecan).
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bioRxiv - Cancer Biology 2023Quote: ... The HLA complexes bound to the beads were then directly eluted into preconditioned Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters, 186002321) using 1% trifluoroacetic acid (TFA; Sigma Aldrich). After two washes of 1mL 0.1% TFA ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded according to FUJIFILM Cellular Dynamics user protocol onto the 96-well plates coated with poly-L-ornithine (P3655, Sigma-Aldrich) and laminin (L2020 ...
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bioRxiv - Neuroscience 2023Quote: ... 30µl of sample was transferred into a 96-well plate and added 100µl of Hexokinase enzyme reagent (HK) (Sigma-Aldrich, UK), and then mixed and incubated at room temperature for 15mins (Fig ...
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bioRxiv - Plant Biology 2023Quote: Individual 7-day-old Arabidopsis seedlings were transferred to white 96-well plates containing 200 µl reconstitution buffer (2.5 mM MES pH 5.7 (Sigma-Aldrich, Taufkirchen, Germany), 10 mM CaCl2 (Roth ...
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bioRxiv - Bioengineering 2022Quote: ... The mix for the tumour mass was then set into 96-well plates (Corning® Costar®, Sigma-Aldrich, Dorset, UK) and incubated in 37°C for 15 min to allow crosslinking ...
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bioRxiv - Developmental Biology 2023Quote: ... 30 μl was transferred to a 96-well plate and incubated with 100 μl of Free Glycerol Reagent (Sigma F6428-40ML) for 5 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: hα7-nAChR/m5-HT3A chimera-transfected Vero cells (105 cells/mL) were coated onto 96-well plates pretreated with poly-D-Lysine (Sigma-Aldrich), according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Equal volumes of cell suspension were plated onto the centre of the 96-well plates coated with poly-L-ornithine (P- 8638, Sigma-Aldrich). The cells were grown in DMEM/F12 medium (21331–020 ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μL of the diluted cultures were transferred to a 96-well plate and sealed with a Breathe-Easy sealing membrane (Z380059, Sigma Aldrich). Growth at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 105 UFCs from an overnight culture were inoculated in 96-well plates (SPL Life Sciences) containing 200 µL of fresh MH (Oxoid) with doubling concentrations of trimethoprim (Sigma Aldrich). Plates were incubated at 37°C for 20 h without shaking ...
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bioRxiv - Neuroscience 2023Quote: Transfection of all cell lines (SH, NB7 and their Drosha k.o. clones) was performed in TPP 96 well plates (Millipore Sigma # 92696) in 250 μl final volume ...
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bioRxiv - Bioengineering 2023Quote: ... For flow-NMR measurements 250 µL broth were sampled to a 96-deep well plate (DWP) and mixed with 500 µL Acetonitrile (Sigma Aldrich) by pipetting ...
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype control were diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype were control diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Bioengineering 2024Quote: ... 100 µL of sample was transferred to a 96 well plate and 100 µL DMMB solution was added (0.348 mM DMMB (Sigma-Aldrich Ltd.) + 34.78 mM sodium chloride (Sigma-Aldrich Ltd. ...
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bioRxiv - Synthetic Biology 2024Quote: ... Microtiter plates were incubated at 30 °C for the duration of the time course at “high” shake speed setting and linear shaking mode in a 96 well microtiter plate sealed with a Breathe-Easylll transparent membrane (Sigma-Aldrich). Three biological replicates were processed by growthcurver R package and doubling times were found for all strains (Sprouffske and Wagner 2016).
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% (v/v) fetal bovine serum and 1.1%(w/v) methylcellulose (Sigma 94378, Munich, Germany)(Vassella et al. ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed using KRBH Buffer + 1% w/v BSA + 1% v/v Triton X-100 (Sigma). The lysates were sonicated 3 × 10 s in a water-bath sonicator and centrifuged at 10,000 x g for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were pre-treated with 0.01% w/v SDS or 0.1% v/v Triton X-100 (Sigma) with or without Proteinase K ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 1% w/v skim milk with 0.05% v/v Tween 20 (Sigma-Aldrich) in PBS (1% milk PBS-T ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% (v/v) TritonX-100 and protease inhibitor tablet (Sigma). Proteins were immunoprecipitated by incubating cell extracts overnight with either FLAG M2 (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... permeabilized with 0.5% v/v Triton X-100 (Sigma, T8787) in DPBS for 15 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and 0.2% (v/v) Tween-80 (Sigma-Aldrich, MO, USA), and the cells were cultured at 29 °C in cell culture flasks with filter caps ...
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bioRxiv - Developmental Biology 2021Quote: ... 97% (v/v) 2’−2’-thio-diethanol (TDE, Sigma, 166782) was treated for clearing ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1% L-glutamine (v/v) (Sigma Aldrich®, G7513). Cells were cultured at 37°C in a humidified atmosphere containing 5% CO2 ...