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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated in rat anti-somatostatin (1:250, Millipore cat. No. MAB354, RRID:AB_2255365) at 4°C for 48 hours before the addition of primary antibody rabbit anti-c-Fos (1:3000) ...
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bioRxiv - Genetics 2023Quote: ... and rat monoclonal anti-laminin-2 (α2 chain; 4H8-2; Sigma Aldrich; 1:500). Secondary antibodies used were goat polyclonal anti-rabbit Alexa Fluor 594-conjugated (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 1 hour at 4 °C with 2% rat serum (Sigma) to reduce antibody binding to Fc receptors ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used in this study include rat anti-HA (Sigma; 1:1000 dilution), mouse m2 anti-FLAG (1:1,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... as in28 and rat anti-laminin-2 1:100 233 (Sigma-Aldrich, Cat. #L0663). For macrophage staining these included ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with the primary antibody anti-ATE1 (1:200, rat, Sigma-Aldrich), anti- calreticulin (CALR ...
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bioRxiv - Physiology 2023Quote: The following antibodies were used: rat anti-laminin-α2 (1:1500, Sigma-Aldrich, L0663), mouse anti-BA-D5 (MyHC-I ...
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bioRxiv - Cancer Biology 2021Quote: ... anti- Spz (rat, 1:50, requested from Satoshi Goto), Anti-Elav (rabbit, 1:50, DSHB) and Caspase 3 (rabbit, 1:500, Sigma Aldrich). Samples were washed four times for 10 minutes each in PBST and incubated at room temperature in secondary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... loaded to a polyacrylamide gel (30 μg of total protein/lane) and after gel electrophoresis and protein transfer to polyvinylidene difluoride (PVDF) membranes (Millipore, cat. no. IPVH00010, Burlington, MA). The membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PVDF, Millipore, IPVH00010). The membranes were incubated in blocking buffer (5% w/v nonfat dry milk in Tris-buffered saline ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by 8% to 12% SDS–PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Blocking and probing with antibodies were performed as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins (25-50µg) were resolved on 8 to 15% SDS-PAGE and blotted onto PVDF membrane (Millipore). Membranes were blocked for 1 h at room temperature with TBS-0.1% Tween20 and 5% milk or 1% bovine serum albumin (BSA) ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
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bioRxiv - Immunology 2020Quote: ... then 25 ug total protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk and probed with the indicated primary antibodies following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... Separated proteins were transferred to a nitrocellulose membrane and immunoblotted with an antibody recognizing Orai1 (Sigma Aldrich). Each experiment was performed at least 3 independent times and analysed with the program ImageJ (NIH ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were run on SDS-polyacrylamide gel and transferred to Immobilon-P membrane (Millipore). Membranes were incubated with anti-YAP (Novus) ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, 0.2 μm pore, Millipore Corp., MA, USA) and incubated overnight at 4°C with primary antibody solution containing polyclonal rabbit anti-mouse ApoA-II antiserum (diluted 1:3000 ...
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bioRxiv - Plant Biology 2019Quote: ... following which the proteins were transferred from gel to membrane and probed using Anti-FLAG antibody (Sigma).
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bioRxiv - Physiology 2020Quote: ... Equal quantities of proteins were resolved by SDS-PAGE gels and blotted onto polyvinylidene difluoride membranes (Millipore). Then ...
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bioRxiv - Microbiology 2019Quote: ... Total protein (30 μg) was separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Merck, Germany) using a transfer apparatus (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... Equal amounts of total protein were resolved by SDS-PAGE and transferred onto a PVDF membrane (Millipore). The membrane was then blocked with horse blocking buffer (bioWORLD ...
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bioRxiv - Cell Biology 2019Quote: Proteins separated by SDS-gel electrophoresis (7.5–15% polyacrylamide) were transferred to Immobilon-PSQ PVDF membranes (Millipore). After blocking with 5% non-fat dry milk in TBS (50 mM Tris·HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved on SDS-polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Immobilon-P, Millipore). After being incubated with the primary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... followed by the transfer of proteins to polyvinylidene difluoride (PVDF) membranes (EMD Millipore, catalog # IPVH00010, lot # R0HB88840).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS acrylamide gel electrophoresis and transferred to IMMOBILON-FL 26 PVDF membrane (Millipore) probed with the indicated antibodies and visualized either by chemiluminescence (according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of protein lysate were separated by SDS-PAGE and transferred onto a nitrocellulose membrane (Millipore) at 30 V for at least 60 min (overnight for ubiquitination assays) ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg of proteins were separated by 10% SDS-PAGE and transferred to Immobilon-FL membranes (Millipore). For dot-blot assays ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated by SDS–PAGE (4-20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2022Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were separated in SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then transferred to a Amersham™ Protran® Western-Blotting-Membrane (#GE10600044, Sigma-Aldrich) at 120 V for 1 h ...
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bioRxiv - Biochemistry 2021Quote: Denatured proteins were separated by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore IPFL00010). Membranes were incubated in a casein-based blocking buffer (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck Millipore, Burlington, MA) by electroblotting ...
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bioRxiv - Microbiology 2021Quote: ... Equivalent protein amounts were resolved by SDS-PAGE and subsequently transferred to Immobilon PVDF-FL membranes (Millipore). Blots were probed using mouse anti-Flag M2 (1:3000 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins (20 μg per lane) were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore). Membranes were blocked in 4% milk in TBST (0.05M Tris pH7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equivalent amounts of protein per sample were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk or 5% BSA in TBST (Tris-buffered saline containing Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... Identical amounts of protein samples were subjected to SDS-PAGE and transferred to Immobilon-FL membrane (Millipore). Immunoprecipitated samples were subjected to SDS-PAGE and transferred to Immobilon-FL membrane (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... 20-30 μg protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore, USA). After being blocked with 5% non-fat milk for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Equal amounts of protein (20ug) were loaded onto a 10% polyacrylamide gel and 0.45μM nitrocellulose membrane (Millipore, Billerica ...
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride membranes (PVDF, Merck Millipore) for immunoblot analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10% SDS-PAGE gels followed by transfer to polyvinylidene difluoride Immobilon membranes (Millipore). Phospho-Erk was detected using a phospho-Erk antibody (clone 4695P ...
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bioRxiv - Neuroscience 2020Quote: ... The protein extracts were run on a 5%–12% gel and transferred to nitrocellulose (NC) membranes (Millipore). After blocking in a milk solution (5% powdered milk in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... equal amounts of protein were subjected to SDS polyacrylamide gel electrophoresis and transferred to PVDF membrane (Millipore) by wet transfer ...
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bioRxiv - Biochemistry 2021Quote: ... The separated proteins were transferred by electrophoresis to polyvinylidene difluoride transfer membranes (Immobilon-P, Millipore, MA, USA). The membranes were treated with diluted affinity-purified anti-p-eIF2α (p-eIF2α ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were separated by SDS-PAGE and then transferred to a PVDF membrane (Millipore, Burlington, Massachusetts). Membranes were stained with 1:1000 dilution of Anti-SARS-CoV SΔ10 antibody ([1A9] ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were run in SDS-PAGE and transferred to Immobilon Polyscreen PVDF transfer membranes (#IPVH00010, Merck Millipore) or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: The indicated proteins were loaded to SDS-PAGE gel and transferred to methanol-activated PVDF membranes (Millipore), which were blocked in 5% non-fat milk containing 0.2% Tween for 1 h at room temperature (RT) ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...