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Citations for Millipore Sigma :
1501 - 1550 of 2421 citations for RPL3L Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Protein elution was carried out under native conditions by competition with commercially available 3× FLAG peptide (Sigma). Eluted proteins separated in 4-20% polyacrylamide gels in denatured conditions were visualized for purity after Coomassie blue (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides from both consecutive digestions were recovered by solid phase extraction using C18 ZipTips (Millipore, Cat# ZTC18S096), and resuspended in 0.1% formic acid for analysis by liquid chromatography-mass spectrometry (LC-MS/MS) ...
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bioRxiv - Cell Biology 2023Quote: ... Following primary antibodies were used for IHC and IF: mouse anti-2A peptide (Millipore, MABS2005, 1:200), rabbit anti-Active YAP1 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant protein was then eluted in lysis buffer containing 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich) for 1 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and each peptide fraction was then cleaned up using 2 ug capacity C18 ZipTips (Millipore, Billerica, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Peptides collected for the DIA spectral library were desalted with ZipTips C18 column (EMD Millipore, Burlington, VT), dried in speedvac and resuspended in 300µl of 0,1% TFA ...
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bioRxiv - Molecular Biology 2023Quote: ... Complexes were eluted with 5 CVs of wash buffer supplemented with 150 µg/mL 3xFLAG peptide (Sigma). Eluted protein was analysed by SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... To generate CD8+ T lymphoblasts responsive to ovalbumin peptide (OVAp; SIINFEKL OVA257–264, Cat# S7951, Sigma-Aldrich), T cells were incubated for 48h with OVAp (10 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were eluted twice (15 min. at RT) by incubation with 3x-FLAG peptide (Sigma #F4799) at a concentration of 100 mg/mL in PBS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 4 h in presence of SIINFEKL peptide + 10 µg/ml of brefeldin A (Sigma-Aldrich). Specific CD8+ T cells were evaluated by incubation with SIINFEKL257-264-APC-Labeled or SIINFEKL257-264-BV421-Labeled tetramers (NIH Tetramer Core Facility at Emory University) ...
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bioRxiv - Microbiology 2024Quote: ... was utilized to measure the binding kinetics of three peptides to lipid A (L5399, Sigma, Shanghai, China). Peptides were diluted with 75 mM NaCl solution at concentrations of 100 ...
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bioRxiv - Microbiology 2024Quote: ... Bound material was eluted by incubating the beads with HA peptide (Sigma Aldrich, St. Louis, MO, USA) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and saturated with blocking solution for 30 min before immune-staining with Duolink by using PLA Technology (Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with blocking solution containing 10% normal donkey serum (DNS) and 1% Triton X-100 (Sigma-Aldrich) in PBS with 0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed with 0.2% gluteraldehyde/4% PFA at room temperature and incubated overnight in hybridization buffer (5% Dextran sulphate, 2% blocking powder from Roche, 5X SSC, 0.1% TritonX, 0.1% CHAPS from Sigma Aldrich, 50% formamide ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed in PBS overnight, incubated with a blocking solution (PBS, with 0.5% Triton X-100 (Sigma-Aldrich) and 10% goat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... plates were blocked with 200 μL/well blocking buffer containing 1% (w/v) Bovine Serum Albumin (BSA, Sigma Aldrich) in PBS for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed with PBS and incubated for 2 h in blocking solution (0.1 M PBS, 0.25% Triton, 5% normal goat serum, Sigma Aldrich). Tissue was treated with primary antibody ...
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bioRxiv - Cancer Biology 2019Quote: Immunoblots were performed as previously described [23] using 5% milk powder for blocking and developed using ECL (Sigma-Aldrich). Details of the antibodies used in Additional file 1 ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated for 1-3h in blocking solution (10% v/v Sigma-Aldrich sheep serum in PBS-T) at 21°C ...
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bioRxiv - Genomics 2019Quote: ... the blocking solution was aspirated and primary antibodies were added (mouse anti-GFP antibodies [Sigma] at 1:100 dilution). After incubation for 1 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: Sections were incubated in a permeabilization and blocking solution of 0.3% Triton X (A4975, PanREAC AppliChem) and 3% BSA (A7906, Sigma) in PBS for 24 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... BBMV were centrifuged and resuspended in binding buffer (20 mM Tris, 150 mM NaCl, 1 mM MnCl2, pH 7.4, 0,04% Blocking reagent from Sigma Aldrich). Competition binding experiments were conducted by incubating 1.4 μg of BBMV protein with 0.65 nM 125I-Vip3Aa in a final volume of 0.1 ml of binding buffer for 90 min at 25°C in the presence of increasing amounts of unlabeled Vip3Aa ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Membranes were blocked with Odyssey blocking buffer in PBS (Li-COR) and incubated with anti-FLAG M2 (F1804; Sigma) and anti-c-Myc (C3956 ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated in blocking reagent and then with FLAG M2 antibody (1:100 dilution, Sigma-Aldrich #1804) at 4 °C overnight ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... an additional blocking step was performed by incubating cells with 2 mg/ml γ-globulins from human blood (Sigma) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Blocking and permeabilization was performed with 10% goat serum prepared in PBS with 0.25% Triton X-100 (Sigma-Aldrich). Cells were incubated with anti-PDGFRβ (28E1 ...
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bioRxiv - Neuroscience 2021Quote: ... and blocked for 1 hour at room temperature in blocking solution (1% (w/v) bovine serum albumin (Sigma A9418), 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed gonads were rehydrated in PBST (PBS and 0.1% Tween 20) and blocked with Roche Blocking reagent (Sigma 11096176001) for 35 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Sections were defrosted at room temperature and subjected to epitope retrieval at 95 ºC for 30 min followed by a blocking step at room temperature for 30 min with a solution containing 0.01% Triton X-100 (Sigma) and the appropriate 10% normal serum in PBS ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Biophysics 2022Quote: ... permeabilization step was followed by an hour of incubation with blocking buffer comprising 1% bovine serum albumin solution (Sigma) with 0.1% tween (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Five floating sections per mouse were incubated in a blocking solution composed of 10% normal goat serum (NGS; Sigma) and 1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: Brain sections were washed with 0.1M phosphate buffer (PB) and incubated with blocking solution containing donkey serum (5%, Sigma) in PB for 60 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h.The membrane was incubated overnight in Pierce SuperBlock Blocking Buffer (Pierce #37353) with 0.1% Tween 20 (Sigma #P7949) and validated primary antibodies ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Neuroscience 2022Quote: Brain sections were first incubated for 1h at room temperature (RT) in a blocking solution containing 1% BSA (Sigma), 2% Donkey Serum (Biowest ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were incubated with the secondary antibody (1:1000 in blocking solution) and 1µg/ml DAPI (#D9564, Sigma) for 2 hours at room temperature (RT) ...
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bioRxiv - Genetics 2021Quote: ... in PBS for 20 minutes at 4°C followed by a 1 hour room temperature incubation in blocking solution of 5% donkey serum (Biosera) in 0.3% Triton-X-100 (Sigma) in PBS (0.3% PBST) ...
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bioRxiv - Cancer Biology 2021Quote: ... washed again with PBS followed by a blocking step for 1 hr with PBS + 0.1% Tween-20 (Sigma-Aldrich), 2% BSA (PAA Laboratories GmbH ...
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bioRxiv - Neuroscience 2020Quote: Sections were washed in 0.1M PBS solution before blocking by incubation with 3% normal donkey serum (NDS, Sigma; D9663), 0.2% PBS-Triton for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... the cryosections were rinsed in PBS for 5 min and incubated with blocking solution (5% Normal Donkey Serum [Sigma-Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... for 30 minutes at room temperature and non-specific blocking with 1% bovine serum albumin (BSA; A2153, Sigma-Aldrich) and 10% normal goat serum (NGS ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were then treated with a permeabilization and blocking solution comprised of 0.3% Triton X (PanREAC AppliChem) and 0.5% BSA (Sigma–Aldrich) in PBS for 30 m ...