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Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for 6 Chloro 3 methyl 4 pyridinemethanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: C57BL/6N female mice (4-6 weeks old) were superovulated by injection with 5 IU of pregnant mare serum gonadotropin (PMSG) (Millipore), followed 5 IU of human chorionic gonadotropin (hCG ...
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Microbiology 2020Quote: ... and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Cover slips were placed over the samples and kept at room temperature for ∼ 30 minutes before cells were imaged.
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bioRxiv - Physiology 2020Quote: ... Dead cells and debris were excluded by measurements of forward-versus side-scattered light and DAPI (4′,6-diamino-2-phenylindole) (Sigma) staining ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... After incubating in primary antibodies for two hours and DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride, Sigma Aldrich, 10,236,276,001 Roche) for the last ten minutes ...
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bioRxiv - Cell Biology 2021Quote: NCMs were seeded at a density 4 or 8 × 105 cells/well in 6-well plates coated with 0.01% of collagen (Sigma-Aldrich) and cultured in a medium containing DMEM/Medium199 (4:1) ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue and cells were incubated in 2-(4-amidinophenyl)-1H -indole-6-carboxamidine (DAPI, D-6564, Sigma, 1:1000). Biotinylated-secondary antibodies were from Vector Labs ...
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bioRxiv - Immunology 2022Quote: ... For cell sorting cell suspensions were stained for viability with 1:3000 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) immediately before sorting.
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bioRxiv - Developmental Biology 2022Quote: ... sections were washed in PBS and then incubated for 10 minutes in 1:10000 4’,6-diamidino-2-fenilindol (DAPI; Sigma) for nuclei counterstaining ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing sections were incubated 10 min at room temperature (RT) with 0.001 % DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma, D9542) in PBS for nuclear staining ...
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bioRxiv - Immunology 2022Quote: ... The sections were washed in PBS and incubated 10 min with 4’,6-diamidino-2-phenylindole (DAPI, 1/10,000, Sigma-Aldrich) to visualize the cell nuclei ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were washed three times with 1x PBS and incubated for 5 minutes in 300 nM 4′,6-diamidino-2-phenylindole (Sigma) before a final 1x PBS wash ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were further incubated for 10 min at room temperature with DAPI (4’,6-diamidino-2-phenylindole, 1:5000, Sigma). Incubation with the secondary antibodies lasted for 3h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... and resuspended in 50 to 80 µl FC buffer containing 0.1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Primary tumor samples were stained with CD45-APC-Cy7 (Biolegend ...
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bioRxiv - Developmental Biology 2020Quote: ... Next the slides were mounted with a coverslip using Mowiol (6 g glycerol, 2.4 gr polyvinylalcohol 4-88 (Sigma, 81381), 6 ml MQ and 12 ml 0.2 M Tris HCL pH 8.5).
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were washed 5 times in PBT and incubated for 2h at RT with secondary antibodies and 4’,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma) (1µg/mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... An incubation was done 45 min at room temperature with a mix of 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, 1µg/mL, 10236276001, Sigma-Aldrich) and the secondary antibody Donkey anti-Mouse Alexa Fluor 555 in PBS 1X ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... RIPK3 inhibitor GSK 872 (N-(6-(Isopropylsulfonyl)quinolin-4-yl)benzo[d]thiazol-5-amine) was purchased from Millipore Sigma and frozen stocks were maintained in DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... then stained in 1x PBS + .5M NaCl with 50 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich #D9542).
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bioRxiv - Physiology 2021Quote: ... 6 × 105 cells were seeded on 6-well multiwell plates and counted every two days from day 4 to day 14 using Trypan blue (Sigma) and a Neubauer chamber.
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... inactivated with 10 µg/ml mitomycin C and seeded at 4 × 105 per 6 cm dish) in DMEM/F12/KSR medium (D-MEM/F12 (Sigma) supplemented with 20% KSR additive ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in medium with 10% fetal calf serum and 1 × 105 cells were cultured for 4 to 6 days with different concentrations of ConA (Sigma) or co-cultured with (2.5 × 104 ...
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bioRxiv - Neuroscience 2021Quote: ... a pool of 4 brains was used (n = 6) and mixed with 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). The homogenate was centrifuged at 2400 g for 10 min at 4 °C and the supernatants were collected for the analyses of the following parameters ...
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bioRxiv - Biochemistry 2022Quote: ... a total of 0.4 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Sigma) and cells were fixed 4 days later ...
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bioRxiv - Immunology 2022Quote: ... The sections were then washed with PBS/0.5% Triton and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich). Digitally scanned sections were analysed using Image Scope software (Scanscope XT ...
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bioRxiv - Immunology 2022Quote: ... Progenitors were spinfected in 6-well plates at 23-32°C for 30 minutes at 1000 x g with 4 µg/mL polybrene (Sigma) and 1 µM β-estradiol at 0.25 × 106 cells/mL for single gene knockouts ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were washed several times in PBS and incubated with 1 µg/mL of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) to visualize the nuclei.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Chromosomes were stained with 4′,6-diamidino-2-phenylindole at a concentration of 5 μg/ml (DAPI; Sigma-Aldrich, D8417).
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... in PBS for 2 h at room temperature and counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Sigma-Aldrich) to identify cell nuclei.
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bioRxiv - Neuroscience 2024Quote: Pharmacological experiments were carried out on PCs during simultaneous somatic and dendritic recordings after 10 minutes of control recording using ACSF with the following drugs: 20 µM 4- (N-ethyl-N-phenylamino)-1,2 dimethyl-6-(methylamino)pyrimidinium chloride (ZD7288) (Sigma-Aldrich), or 1 µM TTX ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT cells were plated in 10-cm dish with culture medium containing only blasticidin S following co-transfection with Flp recombinase expressed plasmid pPGKFLPobpA and tetracycline-responsive plasmid pcDNA5FRT/TO containing TDP-43 Q331K or pcDNA5FRT/TO only as isogenic sham control in the ratio 6:4 using PEI (polyethyleneimine; Sigma) transfection method ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... i.p.) young adult (4-week-old) C57BL/6 male mice and transferred to ice-cold DMEM/F12 media (Sigma Aldrich) in 6 well plates ...
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bioRxiv - Immunology 2024Quote: ... slides were washed in PBS and incubated in 5 μg mL-1 4’,6-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) in PBS (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Biochemistry 2022Quote: ... cells were mounted in Sigma-Aldrich DuoLink in situ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich), allowed to incubate at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
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bioRxiv - Immunology 2023Quote: 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to exclude dead cells and debris (Sigma Aldrich, St. Louis, MO). All antibodies are from Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... Gene mutation was induced 4 days after organoid passage during 6 days with 400 ng/ml doxycycline (D9891, Sigma-Aldrich). When indicated ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS and the cells’ nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL, Sigma-Aldrich) for 5 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...