Labshake search
Citations for Millipore Sigma :
1501 - 1550 of 10000+ citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: We used tartrazine (T0388, industrial grade, ≥85%) and 4-aminoantipyrine (4-AA, A4382, reagent grade) sourced from Sigma Aldrich, along with low-melting-point agarose (A9414 ...
-
Anisotropic Rod-Shaped Particles Influence Injectable Granular Hydrogel Properties and Cell InvasionbioRxiv - Bioengineering 2021Quote: ... The microparticles were collected into HFE-7500 containing 20 vol.% of 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma) to destabilize the surfactants ...
-
bioRxiv - Bioengineering 2022Quote: ... Slides were then placed into an anhydrous ethanol solution (9.5 mL) containing trichloro-1H,1H,2H,2H-perfluorooctyl silane (97%, Sigma) (500 μL ...
-
bioRxiv - Bioengineering 2022Quote: ... The wafer with the etched pattern underwent trichloro (1H, 1H, 2H, 2H-perfluorooctyl) silane (Sigma Aldrich, St. Louis, MO) treatment for 24 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... The wafer was then plasma-activated and silanized with vapored trichloro (1H,1H,2H,2H-perfluorooctyl) silane (Sigma-Aldrich) overnight ...
-
bioRxiv - Bioengineering 2022Quote: ... Afterward, the pre-mold was silanized with 2 μL of trichloro (1H, 1H, 2H,2H-perfluorooctyl) silane (Sigma Aldrich) and incubated for 1 h at 80°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... The recovered emulsion was then broken with 100 μL of 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich), and the aqueous phase was mixed with 100 μL of 200 μg/μL total yeast RNA (Ambion).
-
bioRxiv - Cell Biology 2020Quote: ... then rinsed 3 times in PBS (5 minutes each) and embedded in 4% low gelling temperature agarose in PBS (Sigma-Aldrich A0701). 55 μm slices of the ovaries were cut with a vibratome (Leica VT 1000 S ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4 μg of pSC2G-CFTR23 plasmid was co-transfected with 1 μM single-stranded DNA repair template (synthesized by Sigma, Table 3) comprising the CFTR-W1282X and silent protospacer adjacent motif (PAM ...
-
bioRxiv - Developmental Biology 2020Quote: ... Wild-type ICR females (3-4 weeks old) were super-ovulated by sub-cutaneous injection of pregnant mare’s serum gonadotropin (PMSG) (Sigma-Aldrich, Rehovot, Israel) (5 units ...
-
bioRxiv - Microbiology 2020Quote: ... primary antibodies were mouse-anti-ROP2/3/4 (1:250 dilution, 4A7, (58)) and rat anti-HA (1:500, clone 3F10, Sigma Aldrich, 11867431001), and secondary antibodies were goat anti-mouse IgG-Alexa 647 (1:2000 dilution ...
-
bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
-
bioRxiv - Molecular Biology 2020Quote: ... The bound probe was visualized using 5-bromo-4-chloro-3 indoyl phosphate (NBT/BCIP; Sigma-Aldrich Corp., St. Louis, MO, USA). Bright field images were captured on the A1+ Nikon confocal microscope fitted with a DS-Ri1-U3 color camera at 20x magnification and 4076×3116 pixel resolution ...
-
bioRxiv - Cell Biology 2020Quote: ... The liver tissue was cut into 3-4 mm pieces and incubated with 1 mM EGTA (Sigma-Aldrich, Cat. no. E0396-10G) in 1 x DBPS (Gibco™ ...
-
bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a tip resistance of 3 to 4 MΩ were filled with (in mM): K-gluconate (135 mM, Sigma; G4500-100G), EGTA (0.2 mM ...
-
bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Surgical specimens were washed twice in phosphate buffered saline (PBS) and fixed for approximately 3 hours in 4% paraformaldehyde (Sigma, St. Louis). Tissue was washed two times in PBS and incubated at 4°C for 3-5 days in 30% sucrose ...
-
bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
-
bioRxiv - Biochemistry 2020Quote: ... were pooled together (6 mL) and concentrated/buffer exchanged using an Amicon® Ultra-4 Centrifugal Filter Units (3 kDa, Millipore, Ireland). The final volume of the purified bacteriocin protein fraction was reduced to 200 μL ...
-
bioRxiv - Plant Biology 2020Quote: ... Detection was performed by adding 200 μl of western blue substrate (SIGMAFAST™ BCIP®/NBT = 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium, Sigma) followed by incubation at room temperature in a dark and moist environment until the reagent’s color became visible ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated for 3 days at 37°C before overlay medium was removed and cells were fixed with 4% PFA (Sigma-Aldrich, MA) for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... COMMD2 immunoblots were blocked for 1 hr at room temperature in 3% milk TBST and probed overnight at 4°C with the primary antibody (Sigma ref# HPA044190) suspended in 3% milk TBST ...
-
bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
-
bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
-
bioRxiv - Neuroscience 2023Quote: ... Fixed cells were incubated with staining solution (1mg/mL of X-Gal,5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside; Sigma Aldrich #11680293001)(Lorenzo ...
-
bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
-
bioRxiv - Immunology 2024Quote: ... Peritoneal macrophages (PerMacs) were collected from the peritoneal cavity via gavage 4 days after intraperitoneal injection of 3% brewer thioglycolate medium (Sigma-Aldrich, B2551) and plated in in medium with 10% FBS ...
-
bioRxiv - Genomics 2024Quote: ... Samples were stained with 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542) for 5-10 minutes at room temperature manually and rinsed three times with 1xTBS with 0.1% (v/v ...
-
bioRxiv - Genomics 2024Quote: ... formamide in 1xTBS with 0.1% (v/v) Tween-20 and 3 mg/mL PVSA with 2ug/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, D9542). Readout probes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Buffer on top of cells was collected and further incubated with 3 mM 4-nitrophenyl-N-acetyl-β-D- glucosaminide substrate (Sigma, N9376) for 1 h at 37 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked for a minimum of overnight and up to three days at 4°C in a blocking buffer of filtered 3% bovine serum albumin (Sigma A9418- 50G), 0.02% Tween-20 (Thermo 28320) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Ovary of both medaka and trout were fixed overnight at 4°C by 4% paraformaldehyde (PFA) in 0,01 M phosphate buffer saline pH 7.4 (PBS, Sigma-Aldrich P4417). Ovaries were washed in PBS and conserved in PBS + 0.5% (w/v ...
-
bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by 4% paraformaldehyde in 0.1 M phosphate buffer (4% PFA-PB; pH 7.4; Sigma-Aldrich, St Louis, MO, USA). Lumbar spinal cords were collected and placed into 4% PFA-PB for 2 hours at 4 °C ...
-
bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sma-CreERT2 or Cx40-CreERT2 males were crossed with R26-Confetti females and low doses of 4-Hydroxytamoxifen (4-OHT H6278, Sigma) was injected intraperitoneally to pregnant females in a single injection ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were subsequently fixed in paraformaldehyde (PFA) 4% with 4% sucrose for 10 min and permeabilized with 0,1% Triton X100 (Sigma-Aldrich) for 5 min ...
-
bioRxiv - Neuroscience 2020Quote: 4% hydrogel monomer solution (HMS) was prepared for perfusion by mixing 40% (w/v) acrylamide (4% final concentration; V900845, Sigma), 2% (w/v ...
-
bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated at 37°C for 30 minutes prior to fixation with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Extracted membranes were separated from insoluble debris by centrifugation (130,000 × g for 1 h at 4 °C) and incubated for 2h with 4 mL of Ni2+-NTA beads (Novagen) equilibrated in buffer III (25 mM Tris-HCl ...
-
bioRxiv - Neuroscience 2020Quote: Cells were washed with phosphate buffered saline (PBS) and fixed for 30 min at room temperature with 4% paraformaldehyde (containing 4% sucrose; Sigma). Cells were permeabilized in 0.5% NP40 in PBS for 5 min at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... and 0.26g (12.48 x 10−4 mol, 1:4 stoichiometric ratio) cross-linking agent tetraethyl orthosilicate (TEOS – Sigma-Aldrich, 131903) were mixed at 3500rpm for 1 min using a DAC 150FV2-K speedmixer ...
-
bioRxiv - Cell Biology 2021Quote: ... Chromatin was pelleted by spinning 5 minutes at 10,000 g at 4°C and subsequently incubated for 1 hour at 4°C in HEPES buffer containing 500 units of Benzonase (Millipore) and 2 µg Pol II antibody (ab5095 ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
-
bioRxiv - Neuroscience 2020Quote: ... NestinCreERT2 - /+ TDT +/+ IR fl/fl generated secondary spheres were allowed to grow for 4 DIV and then 0.5 µM of 4-hydroxy tamoxifen (Sigma-Aldrich) or vehicle (ethanol ...
-
bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
-
bioRxiv - Neuroscience 2020Quote: ... Induction of recombination in Ai9 mice electroporated with Cre inducible plasmids was achieved by intraperitoneal injection of pregnant dams with the faster-acting and shorter-lived tamoxifen metabolite 4-hydroxy-tamoxifentamoxifen (4-OHT) (Sigma) (1 mg/20 g of body weight ...
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: Cells were seeded on coated glass cover (Assistent) slips 2 days before fixation with 4 % paraformaldehyde (Sigma, 30525-89-4) for 15 minutes ...