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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Small ribosomal subunit protein uS12 RPS23 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Purified immunoglobulin (Ig) was purified using an antibody purification system based on protein A-affinity chromatography (PURE-1A; Sigma-Aldrich). The manufacturer’s instructions were as described in [8] ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... Lysates were collected by centrifugation and 500 μg of protein were incubated with 0.5 μl of Flag antibody (#F3165, Sigma-Aldrich) at 4 ℃ overnight ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were analysed by standard Western blotting procedures using an HRP conjugated secondary antibody and Immobilon chemiluminescence substrate (Millipore-Sigma). Membranes were imaged on an ChemidocTM MP (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with primary antibodies against protein of interest (NPR, Santa Cruz, sc-39008, 1:100; GFP, Aveslab, GFP-1020, 1:5,000; FLAG, Sigma, F1804 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Plant Biology 2022Quote: ... SDS-PAGE/Western blot analysis was used to identify proteins in the sample with use of anti-FLAG M2 antibody (Sigma) and anti-MYC antibody (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Non-antigen specific IgG and RBD-specific IgG were isolated from serum and eluted RBD-specific antibodies respectively by protein G beads (Millipore). Purified IgG was denatured and treated with PNGase enzyme (New England Biolabs ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed with a free-floating section staining protocol for immunofluorescence using unconjugated primary antibodies (βIV-Tubulin, T7941, Sigma‒Aldrich, dilution 1:400; GFAP, glial fibrillary acidic protein, G-A-5, Sigma‒Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mNG (Vanderbilt Antibody and Protein Resource Core, VU527) (1:1000) or anti-ɑ-tubulin (Sigma-Aldrich Cat# T5168, RRID:AB_477579) (1:5000 ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of proteins was validated by performing a Western blot analysis against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). For the EMSA assays ...
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bioRxiv - Cell Biology 2024Quote: ... and following centrifugation immunoprecipitations were done using supernatant (I mg protein) and anti-FLAG antibody (Sigma #F1804; dilution 1:1000) and Protein G agarose beads (60 µL ...
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bioRxiv - Plant Biology 2024Quote: ... The pull-down ubiquitinated proteins were detected by immunoblotting using an anti-ubiquitin antibody (Millipore Sigma, 05-1307, clone Apu2) at a concentration of 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... 50 µl of the crude extracts were kept for input and 350 µl of the crude extracts were mixed with 30 µl of anti-Myc antibodies (1:5,000 dilution, MBL: M192-3) bound to Protein G Sepharose beads (Sigma- Aldrich) for overnight at 4°C on a nutator (BenchMark) ...
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bioRxiv - Microbiology 2024Quote: ... Cuf1-FLAG protein levels were analyzed using a monoclonal Anti-FLAG M2-Peroxidase antibody (1:5000 dilution) (Sigma, αFLAG-HRP) using a rabbit anti-β-tubulin at 1:500 dilution in 5% milk + TBS-T and 0.02% sodium azide (abcam ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for expression of the fusion proteins by Western Blotting with an α-FLAG M2-HRP antibody (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Molecular Biology 2024Quote: ... HCLE cells were stained with 10 ug of the EcMBP-ABS fusion-protein conjugated with AF594 along with anti-alpha tubulin antibody (Sigma) conjugated with AF488 for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The protein extracts from the tagged lines were probed with anti-FLAG monoclonal antibody (Sigma, #F3165; dilution 1:1 000) and sheep anti-mouse HRP conjugate antibody (NA 931 Amersham ...
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bioRxiv - Biochemistry 2024Quote: ... Transiently expressed 3xFLAG-AAGAB was precipitated using Protein A/G agarose beads and anti-FLAG M2 antibodies (Millipore-Sigma, #F1804) at a final concentration of 0.5 μg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Transiently expressed 3xFLAG-AAGAB was precipitated using Protein A/G agarose beads and anti-FLAG M2 antibodies (Millipore-Sigma, #F1804) at a final concentration of 0.5 μg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... proteins were transferred onto PVDF membranes and probed with antibodies against Vinculin (V9131, 1:1000, Sigma, St. Louis, MO, USA) and LDHA (#2012 ...
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bioRxiv - Cell Biology 2024Quote: ... 1,000 µg of cell lysate was incubated at 4°C overnight with 30 µg of Protein G magnetic beads (Tamagawa Seiki) covalently bound to HA-antibody (Sigma) using BS3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... while GroEL protein levels were measured using a 1:10,000 dilution of anti-GroEL rabbit antibody (Sigma-Aldrich Cat# G6532). Detection of primary antibodies was performed with a fluorescent secondary antibody (Rabbit IgG (H&L ...
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bioRxiv - Neuroscience 2021Quote: ... a small hole was made on the flat end of the egg and either VPA (Sodium Valproate, Sigma Aldrich, 35 μmoles) or vehicle (double distilled injectable water ...
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bioRxiv - Immunology 2021Quote: ... Perfused lungs were cut into small pieces and digested with 1 mg/mL collagenase D (Sigma-Aldrich, St. Louis, MO, USA) and 100 μg/mL DNAase (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated [APOE4/E4]-Ki astrocytes with 100 nM of a small molecule structure corrector for APOE4 named PH-002 34 (Calbiochem, Sigma-Aldrich) during 24 hours ...
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bioRxiv - Immunology 2021Quote: ... tissue from the distal third of the small intestine was flushed with 10% neutral buffered formalin (Sigma, St Louis, MO, USA), then ‘swiss-rolled’ and fixed overnight ...
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bioRxiv - Neuroscience 2020Quote: ... These small tissue squares were then dehydrated with serial alcohol diluations and infiltrated with LR White acrylic resin (Sigma Aldrich L9774), and placed in a gel-cap filled with LR White to polymerize overnight at 50 °C ...
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bioRxiv - Microbiology 2021Quote: Tissue from the lower third of the small intestine was flushed with 10% neutral buffered formalin (Sigma, St Louis, MO, USA), then ‘swiss-rolled’ and fixed overnight ...
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bioRxiv - Microbiology 2021Quote: ... subtilis was achieved by 5 min incubation with small cation specific channel-forming antimicrobial peptide Gramicidin ABC (10 µM; gABC; Sigma Aldrich), and membrane permeabilisation by 5 min incubation with pore-forming lantibiotic Nisin (10 μM ...
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bioRxiv - Cancer Biology 2021Quote: Subcutaneous tumors following 20-30 days of implantation were chopped into small pieces and digested in collagenase (1 mg/mL in DMEM; Sigma-Aldrich) at 37°C for 30 minutes and filtered through a 70-μM cell strainer ...
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bioRxiv - Cell Biology 2021Quote: Primary astrocytes were transfected with either 50 μmol of a HMG20A small interfering (si)RNA or a luciferase siRNA (Sigma-Aldrich) as previously described [17] ...
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bioRxiv - Cell Biology 2022Quote: ... thereafter differentiations were kept in RB- without small molecule inhibitors until day 3 (d3) before the WNT inhibitor IWR-1 (4 μM, Sigma, #I0161) was added for 48 h ...