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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Recombinant Mouse lgfbp4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... difficile spores were incubated with 10 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) with PBS for 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... 20 µM DTT and phosSTOP in the presence of recombinant cyclin A2/cyclin-dependent kinase (CDK)1 (Sigma-Aldrich) and 10 mM ATP incubated for 2 h at 30 °C ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
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bioRxiv - Immunology 2023Quote: ... SRBCs in Alsever’s (TCS Bioscience) were conjugated to recombinant HEL3X (R. Brink) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma) as previously described104 ...
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bioRxiv - Biochemistry 2023Quote: ... Mouse primary cortical cultures were then treated with 0.5 μg/ml human recombinant tissue inhibitor of metalloproteinase-2 (TIMP2, Sigma) for one biological replicate or 10 μM BB94 for two biological replicates ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: Primary rat cortical cultures were treated with recombinant Tat 1-86 (Diatheva, Italy) and with cocaine-HCl (Sigma Chemicals) as described in each Figure Legend ...
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bioRxiv - Developmental Biology 2024Quote: ... as controls and analyzed for promoter activation in the presence of 3mg/l recombinant human SPARC (SRP3159, Sigma-Aldrich) using the Dual-Luciferase® Reporter Assay System (E1910 ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Plant Biology 2023Quote: 6xHistidine-Glutathione-S-transferase (GST) tagged proteins of the predicted intracellular region of SOBIR1 (termed the KD) was cloned into pCDF-Duet vector (Novagen, EMD Chemicals, San Diego, CA) and expressed in E ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Genetics 2020Quote: ... Specific primers for mouse leptin and 2 mouse housekeeping genes used for normalization (β-actin and 34B4 mouse genes) were purchased from Sigma (Sigma, France). We used primers for Leptin (forward ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-phospho-S345 mouse MLKL (Millipore MABC158; clone 7C6.1; 1:2000/1:200), rabbit anti-phospho-S345 mouse MLKL (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... Mouse monoclonal anti-Actin (A5441) and mouse anti-HA (H3663) were purchased from Sigma-Millipore ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Microbiology 2020Quote: ... mouse anti-FLAG (Sigma, F3165) and mouse anti-αtubulin (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... mouse IgG (12-371; Millipore) coupled with beads were incubated with sheared chromatin at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-T7 (EMD Millipore), mouse anti-HA (HA.11 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti FLAG (F1804, Sigma), anti β actin (A-1978 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-FLAG (Sigma F1804) and mouse anti-tubulin (Sigma T5168 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse Flag (Sigma F3165). Secondary antibodies include anti-mouse HRP (Pierce) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-HA (12CA5, Sigma), rabbit anti-Clb2 and guinea pig anti-Sic1 [42] ...
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bioRxiv - Genetics 2021Quote: ... mouse anti-HA (Sigma-Aldrich), mouse-anti-Flag (Sigma-Aldrich) ...
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bioRxiv - Genetics 2021Quote: ... or mouse anti-Flag (Sigma), rabbit anti-H3 antibodies (Abcam).
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bioRxiv - Genetics 2021Quote: ... mouse-anti-Flag (Sigma-Aldrich), mouse anti-VSG3 (Antibody & Bioresource Core Facility ...
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bioRxiv - Neuroscience 2020Quote: ... αFlag-HRP (mouse, A8592, Sigma), αGFP (chicken ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-HA (Sigma, H7411), mouse anti-V5 (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-Rab10 (Sigma, Sab5300028), rabbit anti-phospho-Thr71 Rab29 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-GFAP (Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (Mouse; 1:2000; Sigma), Cre (Rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-NeuN (Millipore; RRID:AB_2298772), mouse anti-CC1 (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-CC1 (Millipore; RRID:AB_2242783), mouse anti-Ermin (Gift from E ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-MAP2 (Millipore MAB3418), Cy3 goat anti-mouse (Jackson 115-165-068) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-GAPDH (Millipore, #MAB374), rabbit anti-LIMP2 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Map2 (Sigma, mouse, 1:200), SAFB1 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse-anti-tubulin (EMD Millipore), mouse-anti-FLAG (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-TUBULIN (Sigma, T6793), mouse anti- HA (Abcam ...