Labshake search
Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Recombinant Human IL34 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
-
Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
-
bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
-
bioRxiv - Molecular Biology 2023Quote: ... or full length DDX50) and 50 ng N-terminally tagged HaloTag-GID4 using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 30kDa MWCO concentrator (EMD Millipore) to 0.2mL ...
-
bioRxiv - Immunology 2024Quote: ... cells expressing GFP-tagged CD80 (Omar S Qureshi et al., 2011) in CHO cell medium (DMEM containing 25 mM D-glucose (Sigma), 1 mM sodium pyruvate (PAA) ...
-
The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
-
bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
-
bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml) and 2i (1μM PD325 ...
-
bioRxiv - Neuroscience 2021Quote: ... the astrocytes were stimulated with TNF recombinant (10 ng /ml) (Sigma-Aldrich Corporation, Missouri, EUA) or MCM (300 µl ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
-
bioRxiv - Microbiology 2019Quote: Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was purchased from Sigma. The protease inhibitor dec-RVKR-CMK was obtained from Tocris ...
-
bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
-
bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
-
bioRxiv - Neuroscience 2021Quote: ... in situ probes were removed using DNAse (Dnase I recombinant; Sigma Aldrich ct. no. 04716728001) as described15 ...
-
bioRxiv - Immunology 2022Quote: 10ug recombinant LCMV glycoprotein in PBS was diluted 1:1 in Sigma Adjuvant System (Sigma) and administered i.p ...
-
bioRxiv - Microbiology 2022Quote: ... High binding 96 well-plates were coated with recombinant fibronectin (2 μg/mL; Millipore Sigma) and laminin (1 μg/mL ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was obtained from Sigma.
-
bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
-
bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
-
bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
-
bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
-
bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
-
bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
-
bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
-
bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
-
bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
-
bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
-
bioRxiv - Biochemistry 2023Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
-
bioRxiv - Cell Biology 2019Quote: Primary human osteoblasts were purchased from Sigma. Co-culture cells were titrated to determine the highest viable seeding density to allow continuous culture for the duration of the osteoclast differentiation assay ...
-
bioRxiv - Molecular Biology 2021Quote: ... 25 IU human chorionic gonadotropin (hCG, Sigma) was injected 76 h later (14:00 on day 4 of estrous cycle ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 nM human gastrin I (Sigma-Aldrich), 500 nM A 83-01 (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... coated with human plasma fibronectin (Millipore Sigma) diluted to 100 μg/mL in PBS ...
-
bioRxiv - Bioengineering 2019Quote: ... Human B-lymphoid (Sigma-Aldrich, Buchs Switzerland), 293T Flp-in T-REX (Life Technologies ...
-
bioRxiv - Immunology 2019Quote: ... 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum ...
-
bioRxiv - Developmental Biology 2020Quote: ... and human chorionic gonadotropin (hCG) (Sigma Aldrich). Fertilized oocytes were then transferred into M2 medium (Millipore ...
-
bioRxiv - Bioengineering 2019Quote: ... human tenascin-C (Millipore Sigma, Catalog #CC065), and human plasma fibronectin (Millipore Sigma ...
-
bioRxiv - Cancer Biology 2019Quote: ... 5 µg/mL human transferrin (Sigma-Aldrich), 92 nM FeCl3 (Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Human embryonic kidney (HEK) 293T cells (Sigma) were maintained in DMEM supplemented with 10% fetal bovine serum ...
-
bioRxiv - Biochemistry 2020Quote: Human neuroblastoma SH-SY5Y cells (Sigma-Aldrich) were grown in a 1:1 mixture of minimal essential medium (HyClone ...
-
bioRxiv - Cancer Biology 2019Quote: ... and Human Adipocyte Magnetic Bead Panel (Millipore), a Bio-Plex 200 plate reader and the Bio-Plex Manager 6.1 software (Bio-Rad Laboratories) ...
-
bioRxiv - Neuroscience 2020Quote: ... goat anti-human IgG-peroxidase (Sigma-Aldrich), and mouse monoclonal anti-NeuN (clone A60 ...