Labshake search
Citations for Millipore Sigma :
1451 - 1500 of 2423 citations for OAT Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... Samples were rinsed with 1x PBS three times and incubated with blocking buffer (0.5% BSA (Sigma-Aldrich A7030) in 1x PBS ...
-
bioRxiv - Immunology 2023Quote: ... during 5 minutes at RT followed by blocking with 10 μg/mL human γ-globulin (Sigma-Aldrich, USA) in TBS-0.5% blocking reagent (Roche ...
-
bioRxiv - Cell Biology 2023Quote: ... Sections were then blocked for 1 hr in blocking solution (DPBS containing 10% goat serum (Sigma Aldrich, G9023), and 0.3% Triton X-100) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... membranes were soaked in blocking solution +0.02% Tween 20 with 0.15 ug/ml GFP antibody (Millipore-Sigma 11814460001) for 30 min ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... membranes were soaked in blocking solution +0.02% Tween 20 with 0.15 ug/ml GFP antibody (Millipore-Sigma 11814460001) for 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... The sections were treated with blocking buffer (TEM-BB) composed of 1% fish skin gelatin (Sigma-Aldrich / Merck) dissolved in 0.1M HEPES for 1 hour ...
-
bioRxiv - Neuroscience 2023Quote: ... Following blocking for 30 min in PBT (PBS with 0.05% Triton X-100, Sigma Aldrich, 9002-93-1) containing 5% normal goat serum (Sigma Aldrich ...
-
bioRxiv - Neuroscience 2024Quote: ... washed with PBS twice and blocked in Blocking Buffer containing 10% donkey serum (NDS, Millipore-Sigma, S30-M) with 0.3% Triton X-100 (Millipore Sigma ...
-
bioRxiv - Neuroscience 2024Quote: ... washed with PBS twice and blocked in Blocking Buffer containing 10% donkey serum (NDS, Millipore-Sigma, S30-M) with 0.3% Triton X-100 (Millipore Sigma ...
-
bioRxiv - Developmental Biology 2024Quote: ... Embryos were then rinsed in PBSDT and incubated in blocking solution 1× sheep serum (SS, Sigma Cat# S2263) for at least 4 hrs at RT ...
-
eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... the slices were treated with a blocking solution (5 % goat serum and 0.3 % TritonX-100 (Sigma-Aldrich GmbH) in PBS ...
-
bioRxiv - Immunology 2024Quote: ... After cell permeabilization and blocking with 0.1% Triton-X and 1% bovine serum albumin (BSA, Sigma, catalog # 5217), respectively ...
-
bioRxiv - Molecular Biology 2021Quote: ... Exo1-FLAG variants were eluted using wash buffer II containing 0.1 mg/mL 3x-FLAG peptide (Sigma). After applying elution buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2x 21.5 µl (42.5 µl total) of a 200 mg ml-1 dilution of FLAG peptides (Sigma) in 1x TBS was used.
-
miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Labelled peptides were separated on Chromolith Caprod RP-18e HR capillary column (150 × 0.1 mm; Merck Millipore) using linear gradient of buffer B (98% ACN and 0.05% TFA in water ...
-
bioRxiv - Cell Biology 2022Quote: ... The immunoprecipitate was then eluted from the beads by competition with FLAG peptide (Sigma-Aldrich cat #F47799) as follows ...
-
bioRxiv - Biochemistry 2019Quote: ... and eluted with 200 μl of wash buffer supplemented with 0.15 mg/ml 3xFLAG peptide (Sigma-Aldrich). Telomerase quantity ...
-
bioRxiv - Neuroscience 2019Quote: Preadsorption with NPY peptide was carried out to demonstrate the specificity of the anti-NPY antibody (Sigma) used in this study ...
-
bioRxiv - Molecular Biology 2019Quote: ... FLAG-M2 agarose (F2426 or A2220-5ML) and FLAG peptide (F3290-4MG) were purchased from Sigma-Aldrich. RNF219 specific antibodies were produced using an internal (RNF219-A ...
-
bioRxiv - Cancer Biology 2019Quote: ... Peptide mixtures were desalted and concentrated using Ziptip™ μ-C8 pipette tips (Millipore Corp, Bedford, MA). An equal volume of eluted digests was injected at least three times for each sample into Ultimate™ 3000 RSLCnano (ThermoScientific ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The synthesized peptides were purified using 10 μL slurry of anti-FLAG M2 Magnetic Beads (Sigma Aldrich) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
-
bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times and FLAG-tagged proteins eluted using 3X FLAG peptide (Sigma; cat. F4799). Rhodamine-Azide labeling reactions were performed using 1.7 mM TBTA ...
-
bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich). E-buffer (20 mM Na·HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... the beads were resuspended in 50µL of FLAG peptide solution (1µg/µL in RIPA, Sigma-Aldrich, F3290) after the last wash to elute FLAG-tagged Arc and other proteins from beads ...
-
bioRxiv - Cell Biology 2021Quote: ... grown for 2.5 hours and arrested by adding 3 μg/ml α-factor (Sigma, custom peptide WHWLQLKPGQPMY) every 60 min for 120 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... proteins were eluted twice with 2 ug/uL V5 peptide in water (Sigma-Aldrich, St. Louis, MO) at 37°C for 15 minutes with shaking at 1000rpm ...
-
bioRxiv - Biochemistry 2021Quote: ... tryptic peptides were acidified to a final concentration of 2% (v/v) with formic acid (Sigma Aldrich) and desalted using StageTips (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... the digested peptides were alkylated with 20 μL iodoacetamide (5 mg/mL) (Life Science, Sigma-Aldrich, GmbH) and incubated for 30 min in the dark ...
-
bioRxiv - Molecular Biology 2022Quote: ... Bound proteins were eluted using IP buffer containing 0.25 mg/mL of 3x FLAG peptides (Sigma-Aldrich) at 25 °C for 5 min ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were eluted by the addition of elution buffer containing 3 x Flag peptide (Sigma, cat. #F4799) at 4°C for 30 min ...
-
bioRxiv - Immunology 2020Quote: ... peptide was dissolved in anhydrous dimethyl sulfoxide (DMSO) at 10mg/ml and anhydrous triethylamine (TEA) (both Sigma) was added stoichiometrically to convert the peptide salts into their free forms in order to deprotonate the primary amine from the lysine side chain ...
-
bioRxiv - Neuroscience 2021Quote: ... these cells were cultured in the presence of the OVA257-264 peptide (GenScript RP10611 or Sigma S7951). After 12 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... The phosphorylated peptides were eluted from the beads by incubation with 1.5% ammonium hydroxide solution (Sigma-Aldrich), pH 11.3 ...
-
bioRxiv - Cell Biology 2021Quote: ... The pellets were resuspended in 250 μL of IP lysis buffer with addition of Flag peptide (Sigma), and the mixture was rocked overnight at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Peptide fractions were incubated on a rotating platform with IMAC beads (PHOS-Select iron affinity gel (Sigma)) for 1hr at RT ...
-
bioRxiv - Molecular Biology 2019Quote: ... The FLAG-IP was eluted twice with 300 µl 250 µg/ml 3X FLAG peptides (Sigma, F4799) in TBS (50 mM Tris-Cl ...
-
bioRxiv - Microbiology 2019Quote: ... Peptide hydrolysis for ecClpX and ecClpP was monitored with the substrate Suc-Leu-Tyr-AMC (Millipore Sigma) under the same conditions ...
-
bioRxiv - Genomics 2020Quote: ... Expression of Cas9 was confirmed by Western blot using an anti-2A peptide antibody (Millipore Sigma MABS2005).
-
bioRxiv - Systems Biology 2021Quote: ... Yeast proteins digestion was performed on excised bands from gel gradient and digested peptides of UPS2 (Sigma) were used as external standards for absolute protein quantification (more details in Supp ...
-
bioRxiv - Biophysics 2019Quote: ... and eluted with 5 mL of buffer C containing 0.1 mg/mL FLAG peptide (Sigma-Aldrich, F3290). BLM was further purified using a 1 mL HiTrap SP (GE Healthcare ...
-
bioRxiv - Biophysics 2019Quote: ... and eluted with 5 mL of buffer C containing 0.1 mg/mL FLAG peptide (Sigma-Aldrich, F3290). DNA2 was further purified using a 1 mL HiTrap SP (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... The elutes were combined and the peptides were further purified using C18 ZipTip Pipette Tips (Millipore, USA) as per manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured with overlapping peptide pools at 2LJμg/ml or PHA (Sigma Aldrich, St Louis, MO) at 10 µg/ml as a positive control for 16-18 hours at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... or eluted by competition with Flag peptide at 200ug/ml (five incubations of 5 minutes, P4799, Sigma). Samples were finally concentrated using 3KDa columns (Millipore).
-
bioRxiv - Cell Biology 2021Quote: ... the proteins bound to the beads were eluted by the addition of FLAG peptide (#F3290, Sigma-Aldrich). Proteins in the eluate were separated by SDS-PAGE to detect Tanmp-interacting proteins by Western blotting or by silver staining ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated proteins were eluted with 0.5 mg/mL (diluted in lysis buffer) 3X FLAG peptide (Sigma, F4799) for 30 minutes on a rotator ...
-
bioRxiv - Biochemistry 2022Quote: ... bound proteins were eluted with lysis buffer containing 300 μg/ml Flag peptide (Sigma-Aldrich, Cat# F3290) at 4 °C for 45 min ...
-
bioRxiv - Cell Biology 2022Quote: ... peptides were separated using a gradient from 92% A (0.1% formic acid, FA, Sigma, in deionized water) and 8% B (0.1% FA in acetonitrile ...
-
bioRxiv - Microbiology 2022Quote: ... anti- FLAG M2 magnetic beads were incubated with 100 µg/ml 3x FLAG peptide (Sigma, #F4799- 25MG) in FIB buffer + 10% glycerol for 30 minutes at room temperature ...