Labshake search
Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Integrin Alpha M CD11b ITGAM Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... the NGN2-iNs were quenched with 0.2 M Glycine (Sigma-Aldrich) in PBS for 20 mins and permeabilized with 0.05% Triton X100 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... 1% (v/v) of 1 M HEPES (Sigma-Aldrich, H-4034) at Ph 7.2–7.5 ...
-
bioRxiv - Neuroscience 2023Quote: ... 0.32 M sucrose and permeabilised using 0.5% Triton-X-100 (Sigma). Nuclei were recovered by spinning at 450xg for 5 minutes at 4°C and tagmentation was performed using Illumina Tagment DNA TDE1 Enzyme and Buffer Kits for 30 minutes at 37°C37C ...
-
bioRxiv - Biochemistry 2023Quote: ... m-coumaric acid and methzimazole (MTM) were purchased from Sigma-Aldrich/Millipore (St ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 μL 0.5 M EDTA (0.5 mM final conc. - Sigma, A8806), 899 μL DPBS (no Ca ...
-
bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with 4 M ammonia bicarbonate (Sigma Aldrich), and followed by a standard reduction and alkylation procedures as stated above ...
-
bioRxiv - Biochemistry 2023Quote: ... The dried glycoproteins were reduced using 1,4-dithiothreitol (Sigma; 0.18 M) followed by carboxymethylation using iodacetamide (Sigma ...
-
bioRxiv - Bioengineering 2023Quote: ... and 1 µL of betaine (5 M; Sigma-Aldrich, B0300-5VL), 3.13 µL bovine serum albumin (BSA ...
-
bioRxiv - Plant Biology 2024Quote: ... 50 µL of stop solution (3 M NaOH, ≥98%, Sigma-Aldrich) was introduced to each well ...
-
bioRxiv - Cancer Biology 2024Quote: ... 0.6 μM Carbonyl Cyanide m-Chlorophenylhydrazone (CCCP, mitochondrial uncoupler; Sigma-Aldrich) together with 2 mM pyruvate to achieve maximal respiration ...
-
bioRxiv - Plant Biology 2024Quote: ... Excess staining was removed with 15 M chloral hydrate (Sigma-Aldrich). Samples were moved to 50% glycerol for storage and mounting ...
-
bioRxiv - Biochemistry 2023Quote: ... the cells were harvested and lysed with Cell Lytic M (Sigma). After ultracentrifugation to remove the insoluble fraction ...
-
bioRxiv - Cancer Biology 2024Quote: ... Fixation was stopped using 2.5 M Glycine solution in water (Sigma). Fixed cells were washed with chilled PBS-Igepal (0.5% Igepal CA-630 in PBS ...
-
ERK signaling promotes resistance to TRK kinase inhibition in NTRK fusion-driven glioma mouse modelsbioRxiv - Cancer Biology 2024Quote: ... with 50 ml of 0.15 M tartrate sodium (Sigma Aldrich PHR1409). 10 ml of the Tartaric acid buffer stock were subsequently mixed with 40 ml of Saline Solution (0.9% ...
-
bioRxiv - Neuroscience 2024Quote: ... slices were washed in 0.1 M Tris buffer (TB; Sigma-Aldrich) and permeabilized with 1% Triton X-100 (TX ...
-
bioRxiv - Microbiology 2023Quote: ... The m/z of a GlcNAc-6-P standard (Sigma-Aldrich) was determined as 300.049 with a column retention time of 12.41 minutes (data not shown) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.2 M succinic acid (Sigma Chemical Company, St. Louis, MO, USA) and 2.4 mM nitroblue tetrazolium (NBT ...
-
bioRxiv - Biochemistry 2024Quote: ... 0.2 M lithium acetate (Sigma Aldrich, St. Louis, MO, United States) and 0.1 M dithiothreitol (DTT ...
-
bioRxiv - Developmental Biology 2020Quote: L1 larvae were loosely synchronized (using small scale embryo prep) and treated with 10 μg/mL alpha-amanitin (Sigma A2263), as in Butuci et al ...
-
bioRxiv - Genetics 2020Quote: ... cells were either harvested (for D0 timepoint) or medium was changed to differentiation medium (MEM alpha supplemented with 10% FBS, 50 μg/ml sodium ascorbate (Sigma) and 10 mM β-glycerophosphate (Roth ...
-
bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
-
bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). As for the fibroblasts ...
-
bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
-
bioRxiv - Biochemistry 2020Quote: ... was destarched by incubation with amylase (0.2 mL, Termamyl 120 L, alpha-amylase from Bacillus licheniformis – Type XII-A, Sigma-Aldrich) in sodium phosphate buffer (125 mL ...
-
bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
-
bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
-
bioRxiv - Immunology 2022Quote: ... 0.6μL was deposited on a thin layer of an air-dried saturated solution (0.6μL) of the matrix alpha-cyano-4-hydroxycinnamic Acid (4-HCCA, Sigma Aldrich, France) in pure acetone ...
-
bioRxiv - Immunology 2020Quote: ... and for ChIPseq experiments cells were subsequently fixed in PBS with 1 % paraformaldehyde (Alpha Aesar) and 10 % FBS for 10 minutes at room temperature (reaction was quenched with 125 mM Glycine (Sigma)) ...
-
bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
-
bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
-
bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
-
bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples were rehydrated through the same EtOH series and incubated at 37 °C overnight in alpha-amylase (Sigma-Aldrich A4551), 0.3 mg.mL-1 phosphate buffer 20 mM pH 7.0 ...
-
bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
-
bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
-
bioRxiv - Developmental Biology 2021Quote: ... CST, 4967), Alpha-Tubulin (1/3000; CST, 2144) at +4°C followed by incubating with HRP conjugated anti-rabbit (Sigma-Aldrich) or anti-mouse (CST ...
-
bioRxiv - Molecular Biology 2021Quote: ... Larvae were blocked in 10% sheep serum in PBDT for 1 hour at room temperature and then incubated in mouse anti-alpha acetylated tubulin (Sigma-Aldrich Canada Ltd ...
-
bioRxiv - Immunology 2019Quote: ... Delta-like1 (OP9-DL1) (Schmitt and Zuniga-Pflucker, 2002) was cultured in Minimal Essential Medium Alpha Modification (SAFC Biosciences, Sigma Aldrich) supplemented with 10% (v/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... after that glutamine-free medium in rescue condition was replaced by the medium containing 10% of dialyzed FBS and 2 mM of dimethyl-alpha-ketoglutarate (α-KG) (Sigma). Cells were incubated for additional 24 h and used for clonogenic survival assay.
-
bioRxiv - Cell Biology 2022Quote: ... Collected blebs were then pelleted with a centrifugation at 16100 g for 5 min and incubated in a solution containing an exogeneous ATP regeneration system (energy mix) and alpha-toxin to permeabilise the bleb membrane (5% A-Hemolysin alphatoxin (1 mg/mL; H9395, Sigma Aldrich), 2% energy mix (50 mg/mL UTP ...
-
bioRxiv - Cell Biology 2021Quote: ... Haploid cells were selected in SD-Leu (for Mat alpha selection) and -Arg-Lys with toxic amino-acid derivatives canavanine and thialysine (Sigma-Aldrich) to select against diploids ...
-
bioRxiv - Physiology 2021Quote: ... L4 stage transgenic animals were exposed to chemicals by top-coating with 500 μl of each reagent (alpha-amanitin (Sigma #A2263), cycloheximide (Sigma #C7698) ...
-
bioRxiv - Genetics 2019Quote: ... Membranes were incubated overnight with agitation at 4 °C with 1:2000 monoclonal mouse anti-alpha-tubulin (Sigma-Aldrich; clone DM1A) and 1:1000 monoclonal mouse anti-Cas9 (Biolegend ...
-
bioRxiv - Molecular Biology 2021Quote: ... Successfully transformed cells were cultured in YPD media and eventually arrested in late G1 phase by the addition of alpha factor (Sigma-Aldrich). Cells were washed and resuspended in YPGal to induce the expression of wild-type telomerase ...
-
bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Water (>18.2 MΩ cm-1 at 25 °C) was obtained from an Alpha-Q Ultrapure water system from Millipore (Bedford, MA) and used for purification and HPLC purposes ...
-
bioRxiv - Bioengineering 2021Quote: ... Number 78/07).78 Cells were cultured at 37 °C in a humidified atmosphere at 5% CO2 in minimal essential medium with alpha modification and nucleosides (MEMα; Sigma-Aldrich) supplemented with fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
-
bioRxiv - Neuroscience 2022Quote: ... were seeded at 15,000 cells.cm−2 in 96-well plates and incubated 24 hours later with human Tumor Necrosis Factor alpha (100ng.mL−1) or 0.1% Bovine Serum Albumin (Sigma Aldrich, A0281-10G) in Astrocyte medium (ScienCell ...
-
bioRxiv - Microbiology 2023Quote: ... and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich) in 50% (v/v ...