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Citations for Millipore Sigma :
1451 - 1500 of 3554 citations for Gastric triacylglycerol lipase LIPF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cells were normalized to OD600=0.001 in 1XPBS (phosphate-buffered saline) with 10% of Heat inactivated Fetal Bovine Serum (HI-FBS) (Sigma-Aldrich F9665) and incubated at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant His-tagged proteins were purified from cell lysates on a nickel-chelating column (Ni-nitrilotriacetic acid agarose; His-select high flow nickel affinity gel; Sigma-Aldrich), as described previously [53] ...
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bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich, catalogue No ...
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bioRxiv - Cell Biology 2019Quote: ... IP or His-PD Samples were resolved by SDS-PAGE (10-15% gels) and transferred to Immobilon-P membranes (Millipore Inc.), which were then immunoblotted with the following antibodies against ...
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bioRxiv - Neuroscience 2020Quote: ... was tagged with 6xHis at the C-terminus using PCR amplification and the modified Tau-His cassette was introduced into pET3a plasmid (Novagen, DE) linearized with NdeI enzyme succeeding in pET3a/htau40/6xHis plasmid for the protein expression in E.coli ...
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bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
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bioRxiv - Plant Biology 2020Quote: ... The protein was separated on a 10% SDS-PAGE gel and further analyzed by immunoblotting using anti-His and anti-GST antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were mixed as indicated in the figure and rotated with either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare ...
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bioRxiv - Physiology 2020Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... cells were normalized to OD600=0.001 in 1x PBS (phosphate-buffered saline) with 10% of Heat inactivated Fetal Bovine Serum (HI-FBS) (Sigma-Aldrich F9665) and incubated at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin, Millipore Sigma #5893682001) at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Immunology 2022Quote: ... To prepare HI-WCA-OT-109 copies/ml of bacterial suspension was made up in R10 media: RPMI 1640 (Sigma, R0883) containing 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Plant Biology 2023Quote: ... All ATG3 recombinant proteins (wild-type and Cys-to-Ser mutants) were purified by affinity chromatography on a His-Select Nickel Affinity Gel (Sigma, P6611) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 10% input aliquots were taken and the rest of the sample was incubated with HIS-Select® Nickel Magnetic Agarose Beads (Sigma) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... was concentrated from the flow-through of the second passage through the His-trap column using an Amicon® Ultra Centrifugal Filter (Millipore) with a 30 kDa cut-off ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... 293FT were exposed to supernatants containing pMXs-IRES-Blast-STING-His retroviruses and 4 μg/mL polybrene (Millipore, TR-1003-G). Two days post transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... the soluble fraction (obtained by centrifugation with 13000g at 4°C for 30 min) was applied to His-Select Nickel Affinity Gel (Sigma-Aldrich) in presence of 5 mM imidazole for 2h on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... 1.8 mM KCl and 1.25 mM NaHCO3) before they were transferred to dissociation buffer [HBSS supplemented with 5% heat-inactivated fetal bovine serum (HI-FBS, Sigma, F2442) and 10 mM HEPES (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL from the supernatant and cell lysates were applied on a nylon membrane and two different antibodies (an HRP-conjugated anti-His tag from Qiagen and an HRP-conjugated anti-strep tag from Sigma-Aldrich) were used on separate blots to detect PR3 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissues were mashed through a 70-μm cell strainer in PBS / 2% heat-inactivated fetal bovine serum (HI-FBS; Sigma-Aldrich) / 10 mM EDTA-Na (pH 8.0) ...
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bioRxiv - Physiology 2023Quote: ... Samples were stored at −20°C and insulin secretion was measured using a mouse insulin radioimmunoassay kit (Millipore Cat# HI-14K).
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant containing the nanobody proteins was then purified by immobilized metal-ion affinity chromatography (IMAC) using HIS-Select® Cobalt Affinity Gel (Sigma H8162) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 1:5000 diluted Goat anti-human secondary antibody was added into each well and incubated at 37°C for 1 hr (Goat Anti-Human IgG γ Chain Specific HRP conjugated, species Adsorbed (Human IgM, IgD, and IgA) Polyclonal Antibody for IgG (Cat# AP504P, EMD Millipore, Burlington, MA), Goat Anti-Human IgA ...
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bioRxiv - Immunology 2020Quote: ... 5×105 cells (or 2×106 cells for human bone marrow cells) in 100 μL of PBS including 1% (w/v) BSA were incubated with 10% (v/v) human serum (Sigma Aldrich; for human cells) or 1% (v/v ...
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... and recombinant human epidermal growth factor (EGF; Sigma-Aldrich, E9644) were added to the medium at a concentration of 5 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... human insulin to 10µg/mL (Sigma Aldrich, St. Louis, MO), human epidermal growth factor to 20ng/mL (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... coated with 0.005 mg/ml human plasma fibronectin (EMD Millipore) at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ug/ml human insulin (Sigma-Aldrich, Gillingham, UK). D6 ...
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bioRxiv - Cell Biology 2020Quote: ... fibrinogen (human plasma derived) and WKYMVm were from Sigma Aldrich. Recombinant fNLFNYK ...