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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Sprtn KO was induced by treating 4×105 cells with methanol (MeOH) (vehicle control) or 2 μM (Z)-4-hydroxytamoxifen (4-OHT) (Sigma) for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM NaF, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 1 mM PMSF, 75 mU/ml aprotinin-Sigma-, 50 mM Tris-HCl, pH 7.5) containing 1% TX-100 ...
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bioRxiv - Biochemistry 2020Quote: ... and 4×10^-4 1-Thioglycerol (Sigma Aldrich), and incubated for 72 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-hydroxytamoxifen (4-OHT; 1 μM; #H7904 Sigma) or vehicle was added to the cell culture medium to activate Cre-LoxP recombination ...
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bioRxiv - Microbiology 2020Quote: ... Strains were routinely grown under yeast conditions (media at 30°C) in YPG (1% yeast extract, 2% Bacto peptone, 2% glucose) or under filament-inducing conditions using RPMI medium (Sigma, St. Louis, MO) with MOPS (morpholinepropanesulfonic acid ...
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bioRxiv - Plant Biology 2021Quote: ... total soluble protein was extracted from 50 mg of ground frozen shoot material in 250 µl 2× Laemmli buffer supplemented with 1% (v/v) phosphatase inhibitor cocktail 2 (Sigma-Aldrich, Steinheim, Germany). Proteins were denatured for 10 min at 90°C and separated using 10% (w/v ...
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were infected at low MOI 1-2 in EBM-2 media with supplements in the presence of 8 μg/mL polybrene (Sigma-Aldrich, St.Louis, USA) and spinoculation at 450g for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... zebrafish larvae were injected at 2 dpf with a 1:5 dilution of LC in PBS + 2% phenol red (Millipore Sigma, Cat # P0290) in the caudal vein ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid 3-azido-1-propanol ester chain transfer agent (CTA-azide) were purchased from Sigma-Aldrich (Macquarie Park, Australia). DBCO-modified Ang-2 peptide (TFFYGGSRGKRNNFKTEEYC(Mal-DBCO) ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Biophysics 2022Quote: ... the pancreas was transferred to a foil-covered glass vial containing a 1:1 solution of MeOH (100%) and BABB (1:2 (v/v) benzyl alcohol (402834-500ml, Sigma-Aldrich, Gillingham, UK) to benzyl benzoate (B6630-1L ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Samples were weighed and a digestion solution of 1:1 (vol/vol) nitric acid (Fisher, 7697-37-2) and hydrogen peroxide (Sigma, 7722-84-1) was added to the samples (20-70 mg faecal weight ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated in sucrose buffer (0.1 M Tris-HCl pH = 7.5, 2 mM MgCl2, 1 M sucrose, 1 % Protease Inhibitor Cocktail (Sigma Aldrich), 200 μg.mL−1 RNase A and 10 μg.mL−1 DNase (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Neuroscience 2021Quote: ... and 2 % of FBS for 1 h at room temperature (RT) and incubated with PSD95 (1:1.000, MAB1598; Millipore), PrP (1:500 ...
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bioRxiv - Genomics 2022Quote: ... G418 foci were washed with 1× PBS and fixed using 2% Formaldehyde/0.2% Glutaraldehyde in 1× PBS (Sigma-Aldrich) fixing solution at room temperature for 30min ...
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bioRxiv - Cell Biology 2020Quote: ... 2% horse serum and 1% P/S) supplemented with Gibco™ B-27™ Supplement (50X, 1:50, Sigma) for a further 10 days.
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: Percoll gradients (1-2 per sample depending on harvest density) were formed by vortexing 1 ml Percoll (Sigma P1644) with 110 µl 10x PBS in 2 ml tubes and centrifuging 15 min at 15,000 g ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in the primary antibody (anti-Kv3.2 antibody, 1:200, Cat.no. APC-011, alomone lab, Israel; anti-GAD67 antibody, 1:200, Cat.no. MAB5406, Sigma, USA) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1% Phosphatase inhibitors cocktail 2 Sigma-Aldrich Cat:P5726 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lipids were extracted using a 1:1:2 v/v ratio of methanol (Biosolve) containing 0.01% w/v butylated hydroxytoluene (Sigma), water (Biosolve ...
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bioRxiv - Biochemistry 2022Quote: ... A 1:2 serial dilution (concentration range from 1 mM to 0 mM) of Calcium chloride (#C5080, Sigma-Aldrich), Magnesium chloride (#M2393 ...
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bioRxiv - Biophysics 2022Quote: ... Concentrated Cav1.2(ΔC)/Cavβ3/Cavα2δ-1 sample was immediately incubated with gabapentin (final concentration 2 mg ml-1, 11.7 mM) (Sigma-Aldrich) on ice for four hours prior to cryo-EM sample preparation ...
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bioRxiv - Developmental Biology 2023Quote: Embryos used later than 34 hours post-fertilization were kept transparent by soaking them in embryo medium (Danieau’s Solution) with 1% 1-phenyl-2-thiourea (PTU) (Sigma) to inhibit pigment formation (M. ...
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bioRxiv - Microbiology 2023Quote: ... D-alginate was produced by enzymatically degrading 2% alginate with 1 unit ml-1 of alginate lyase (Sigma Aldrich) at 37 °C for 24 hours ...
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bioRxiv - Plant Biology 2024Quote: ... 0.1% Triton X-100) containing 1% protease inhibitor cocktail (here) and 1% Phosphatase Inhibitor Cocktail 2 (Sigma-Aldrich, P5726). After extraction ...
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl, 1% deoxycholic acid, 0.2% SDS, 2% NP-40 with protease inhibitors, 1 μM ADP-HPD [Sigma 118415] ...
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bioRxiv - Immunology 2024Quote: ... or (E)-2-Benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one (BCI, DUSP 1/6 inhibitor, Cat# 317496, Sigma) at different concentrations as indicated for each experimental condition ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit mAb Phospho-MAPK (Erk 1/2 Thr202/Tyr204) (D13.14.4E) (1:1,000; #4370; Cell Signaling Technology, mouse mAb GAPDH (1;5000, G8795, MERCK/Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Nuclear DNA was counterstained with DAPI (40,6-diamidino-2-phenylindole, 1 mg/ml, diluted 1:12 000; Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral vectors pLKO.1 encoding shRNAs targeting MYCN (TRCN0000020695 for shMYCN.1 and TRCN0000020697 for shMYCN.2, Sigma-Aldrich) were used to knock down MYCN and pMOS028:Mitycam lentiviral vector (Addgene plasmid #163046 ...
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bioRxiv - Biophysics 2022Quote: ... We dissolve 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM) powder (Sigma Aldrich) in HEPES buffer (pH 7.18 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the coupling reagent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich). Stock solutions were prepared in phosphate-buffered saline at concentrations of 140 mM (PDH ...
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bioRxiv - Microbiology 2024Quote: ... CPSs were incubated with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, Sigma-Aldrich). The mixture was then ultrafiltered to remove extra DMTMM and mixed with carboxyl latex beads (2 μm ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Neuroscience 2020Quote: PF-4778574 [N-<(3R,4S)-3-[4-(5-cyano-2-thienyl)phenyl]tetrahydro-2H-pyran-4-yl>propane-2-sulfonamide] was obtained as a powder from Sigma (catalog #PZ0211) and solubilized (2.5 mg/ml ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... Cells were washed with PBS and incubated with 100 µM 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose (2NBDG) (Sigma Cat# 186689-07-6) containing RPMI1640 (HiMedia Cat# AT150 ...
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bioRxiv - Microbiology 2022Quote: ... EIF4E2 shRNA#2 (sh4EHP#2) (Sigma, TRCN0000280916) and EIF4E2 shRNA#3 (sh4EHP#3 ...
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bioRxiv - Microbiology 2022Quote: ... GIGYF2 shRNA#2 (shGIGYF2#2) (Sigma, TRCN0000138937) and GIGYF2 shRNA#3 (shGIGYF2#3 ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5% 2-Mercaptoethanol (Sigma, 60-24-2), and sonicated ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma), 100 U/ml penicillin and 100 µg/ml streptomycin sulfate (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2020Quote: ... with 2% v/v 2-mercaptoethanol (Sigma). The samples are immediately frozen on dry ice and subsequently stored at −80° C until library preparation and sequencing ...
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bioRxiv - Molecular Biology 2021Quote: 2-deoxyglucose (2-DG, Sigma, D6134-250MG) was dissolved in 1x DPBS ...