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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... TRAP staining of osteoclasts was performed on day 6 using a leukocyte acid phosphatase kit (Sigma-Aldrich; #387A). TRAP+ multinucleated cells were scored as mature osteoclasts and quantified ...
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bioRxiv - Immunology 2023Quote: ... 300 µg of each sample was loaded onto an S-Trap mini column and digested using trypsin (Sigma) at 1:20 wt:wt ...
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bioRxiv - Genetics 2022Quote: ... 5’-fluoroorotic acid (5’FOA; Sigma) was used at a concentration of 1 g/L in PMG containing 45 mg/L uracil ...
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bioRxiv - Systems Biology 2020Quote: ... Pressure of the media perfusion was held constant at 10.8 kPa in a microfluidic plate designed to trap haploid yeast (Millipore). To ensure that the yeast cells adapted to conditions within the microfluidic chamber ...
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bioRxiv - Immunology 2021Quote: ... LPS (Escherichia coli serotype 0111: B4) and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Plant Biology 2020Quote: The ability of pollen to remove OH• (generated in a Fenton reaction) was analyzed in experiments with spin trap DMPO (5,5-dimethyl-1-pylloline-N-oxide) (Sigma). First H2O2 and spin trap were added to a suspension of pollen ...
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bioRxiv - Neuroscience 2021Quote: Three-week-old Astrocyte-TRAP mice of both sexes were intraperitoneally injected with PTZ at 60 mg/kg (Sigma P6500 ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were fixed and stained with Tartrate-resistant acid phosphatase (TRAP) kit according to the manufacturer’s instructions (Sigma) to quantify osteoclast numbers ...
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bioRxiv - Cell Biology 2023Quote: ... Sections of 3 μm in the thickness were conducted for Safranin O and fast green and TRAP (Sigma-Aldrich) staining ...
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bioRxiv - Immunology 2023Quote: ... The peptides generated by pepsin digestion were subjected to on-line SPE on a PepMap300 C18 trap column (1 mm x 15mm) and washed with 0.1% FA (Sigma) for 60s ...
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bioRxiv - Immunology 2023Quote: ... osteoclasts were formed and TRAP staining was carried out according to the manufacturer’s instructions (Sigma-Aldrich, Cat. 386A-1KT). Cell counts were determined by capturing three images in the same region of each well ...
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bioRxiv - Cancer Biology 2021Quote: 5-Fluoro-5’-Deoxyuridine (Sigma Aldrich, F8791) and Oxaliplatin (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml of HNO3 5% (Sigma, 84378) were added to each sample and they were placed 1h at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... and 5-PVA (5-phenylvaleric acid, Sigma) were dissolved in equimolar amount of NaOH to prepare a 0.5M stock solution with pH 7.
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bioRxiv - Microbiology 2021Quote: ... 5-aza-cytidine (5-AZAC, Sigma-Aldrich) and 5-deoxy-azacytidine (DAC-5 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5-fluorouacil (5-FU; Sigma-Aldrich) were dissolved in DMSO ...
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bioRxiv - Physiology 2024Quote: ... 5-hydroxytryptamine (5-HT, serotonin) (Sigma-Aldrich) and histamine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 5 × 10−5 M 2-mercaptoethanol (Sigma), and 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... His-Trap fractions containing GFP-nanobody-6xHis were pooled and concentrated using a 10 kDa MWCO centrifugal filter (Amicon Ultra, Millipore). Concentrated GFP-nanobody-6xHis was applied to Superdex S75 Increase 10/300 GL Column connected to an AKTA FPLC (Cytvia ...
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bioRxiv - Cell Biology 2021Quote: ... and RANK-L (100 ng/ml) for 4 to 6 days followed by staining for Tartrate-resistant acid phosphatase (TRAP) using a kit (Sigma) per manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... The combined organic phase was dried using CentriVap Cold Traps (Labconco) and derivatized using bis-(N,N,-trimethylsilyl)-tri-fluoroacetamide (BSTFA; Sigma) as described previously (Franke et al. ...
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bioRxiv - Microbiology 2020Quote: ... 30 million eHAP cells were seeded and transduced with gene-trap retrovirus harvested from three combined harvest in the presence of 8 μg/mL protamine sulfate (Sigma). The transduced cells were expanded and passed for a period of 10 days upon transduction ...
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bioRxiv - Immunology 2020Quote: ... at a density of 50,000 cells/well for indicated time were fixed in 2.5% Glutaraldehyde and Tartrate-Resistant Acid Phosphatase in osteoclasts were stained using TRAP staining kit according to manufacturer’s instruction (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... 30 µl GFP-Trap agarose beads (proteintech) were blocked with 2% (w/v) BSA and 0.1 mg/ml yeast tRNA (Sigma-Aldrich) in lysis buffer for 1 h at 4°C to minimize non-specific interactions ...
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bioRxiv - Biochemistry 2024Quote: ... Tspan12 siRNA duplexes (#1: 5’-GCUUAUCUUUGCCUUCUCCTT-3’ and 5’-GGAGAAGGCAAAGAUAAGCTT-3’; #2: 5’-AUGAGGGACUACCUAAAUATT-3’ and 5’-UAUUUAGGUAGUCCCUCAUTT-3’) (Sigma) using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... and serotonin (5-HT, Sigma-Aldrich, Figure 5) or using blue light on the lumbar spinal cord of ChR2-expressing pups (Vglut2Cre;ChR2floxed ...
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bioRxiv - Neuroscience 2020Quote: ... 5 mM 5-Fluro-2’-deoxyuridine (SIGMA U3003). Cells were transfected at DIV17-19 with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... 20 μM 5-fluorouracil (5-FU, Sigma-Aldrich) was added to prevent progeny growth ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol with addition 5 mM desthiobiotin (Sigma). For CENP-HIKM ...
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bioRxiv - Microbiology 2022Quote: ... 5-fluorouracil (5-FC, Sigma-Aldrich, cat#F6627) was prepared in drinking water at a final concentration of 2.0 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and 5-deoxy-azacytidine (DAC-5, Sigma-Aldrich). In each case ...
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bioRxiv - Neuroscience 2021Quote: ... and 5’-fluoro-2’-deoxyuridine (Sigma, 5 μM) were added to the culture medium at days in vitro (DIV ...
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bioRxiv - Cancer Biology 2020Quote: 5-FluoroUracil (5-FU) (Sigma Aldrich, Milan, Italy) was used at different concentrations and for different time points based on the specific experiment ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 nM 3,3’,5-triiodo-L-thyronine (Sigma), 1 μM dexamethasone (Sigma) ...
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bioRxiv - Genomics 2022Quote: ... 5(6)- carboxyfluorescein (5(6)-FAM) (Sigma Aldrich) (46 ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-hydroxy-L-tryptophan (5-HTP, Sigma-Aldrich); 3-hydroxybenzilhydrazine (NSD-1015 ...
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bioRxiv - Developmental Biology 2022Quote: ... serotonin (5-hydroxytryptamine hydrochloride, 5-HT, Sigma-Aldrich); L-tryptophan (tryptophan ...
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bioRxiv - Cell Biology 2023Quote: ... 5-fluorouracil (5-FU) : 3 µM (F6627; Sigma), cisplatin (CDDP ...
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bioRxiv - Neuroscience 2023Quote: ... 5 mM 5-Fluro-2’-deoxyuridine (SIGMA U3003). Cells were transfected at DIV17-19 with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... For one dose 5-fluorouracil (5-FU, Sigma) treatment ...
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bioRxiv - Cell Biology 2022Quote: ... pLKO.1-puro-CMV-tGFP plasmids targeting SNX5 (5’-ACTATTACAATAGGATCAAAG-3’, 5’-CTGAGTATCTCGCTGTGTTTA-3’) and SNX6 (5’-AGTAAAGGATGTAGATGATTT-3’, 5’-GCCGAAACTTCCCAACAATTA-3’) (Sigma-Aldrich) were lentivirally transduced ...
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bioRxiv - Cancer Biology 2021Quote: ... MYC forward 5’-TGAGGAGGAACAACAAGATG-3’ and reverse 5’-ATCCAGACTCTGACCTTTTG-3’ and GAPDH forward 5’-CTTTTGCGTCGCCAG-3’ and reverse 5’-TTGATGGCAACAATATCCA-3’) (Sigma-Aldrich), the Radian SYBR Green Lo-ROX qPCR Kit (Alkali Scientific ...
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bioRxiv - Immunology 2022Quote: ... The sequences of forward and reverse primers for εGLT (fw: 5′-CTGTCCAGGAACCCGACAGA-3′; rev: 5′-TGCA GCAGCGGGTCAAG-3′) and γ1GLT (fw: 5’-CCAGGGCAGGGTCAGCA-3’; rev: 5’-GGTGCTCTTGGAGGAGGGT-3’) (Sigma-Aldrich) and their corresponding FAM-labelled MGB probes (εGLT ...
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bioRxiv - Biophysics 2023Quote: ... 5 % D2O (Eurisotop) and 5 mM DSS-d6 (2,2-dimethyl-2-silapentane-5-sulfonate, Sigma) as internal reference (Wishart and Sykes ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-GATCCCCAAATCT-3’ and 5’-GATCAGAT[BtndT]TGGG-3’ with 5’ end phosphate (Sigma-Aldrich), were annealed (81 µl of each Oligo 100 µM ...
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bioRxiv - Developmental Biology 2021Quote: ... vorticoides at different developmental stages were incubated in 10-5 M serotonin (5-HT) and 5-hydroxytryptophan (5-HTP) (Sigma-Aldrich, USA) for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’-GACAUAUUUGAUAAACUUAAA-3’, 5’-GUUAUCAGUCUGAGCCAGG-3’), human DRP1 (5’-GCAGAAGAAUGGGGUAAAU-3’, 5’-UCCGUGAUGAGUAUGCUUU-3’, 5’-GAGGUUAUUGAACGACUCA-3’) non-targeting scrambled siRNA (Sigma, SIC001-1NMOL) for 48-72 hours using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Osteoclast generation was verified directly by phase contrast microscopy or after staining for Tartrate-Resistant Acid Phosphatase (TRAP) (Leukocyte Acid Phosphatase kit, Sigma-Aldrich). Human TNFα and CCL2 in M-MØ culture supernatants were measured using commercially available ELISA (BD Biosciences).
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bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted from the other 50 μL of beads (80% of volume for GFP-Trap) using TRI Reagent (Sigma, T9424) and then treated with TURBO DNase (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...