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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Recombinant Mouse Tnf None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged NiV-F and mutants were detected by the anti-FLAG mouse monoclonal antibody (Sigma-Aldrich, F1804) and an Alexa Fluor® 647 conjugated donkey anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... FITC-tagged NeuN antibody (Sigma, MAB377) was prepared following manufacturer’s instructions (Abcam ...
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bioRxiv - Biochemistry 2021Quote: Genes for His-tagged or Strep II-tagged nanobodies were cloned into the pET 26b vector (Novagen). The expression and purification of all His-tagged nanobodies have been described previously 12 ...
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bioRxiv - Physiology 2022Quote: ... Tumor necrosis factor alpha (TNF-α, 20 ng/mL; Sigma-Aldrich) and cycloheximide (chx ...
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bioRxiv - Bioengineering 2021Quote: ... TNF-α (0.5 or 1 µg/mL, Sigma Aldrich, Cat# SRP3177) or lipopolysaccharides (LPS ...
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bioRxiv - Neuroscience 2021Quote: ... or TNF-α and IFN-γ (10 ng/mL; Sigma-Aldrich). In a second set of experiments D3 cells in triplicate wells were treated with 200 μl of the following ...
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bioRxiv - Molecular Biology 2022Quote: ... Western blots of different fractionations during the RNA polymerase immunoprecipitation and polysome gradient fractionation were performed to assay efficiency of flag-tagged RNA polymerase enrichment using the monoclonal Anti-Flag M2 antibody from mouse (F1804, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μg/ml prolactin (mouse recombinant for qPCR, western blot, immunohistochemistry and contraction control; Sigma or Peprotech) or sheep pituitary prolactin for time-lapse and confocal imaging ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Wells were washed thrice with PBST and monoclonal primary antibody (anti-his-tagged mouse antibody, 1:5000 diluted, Sigma-Aldrich, USA) was added and incubation was continued for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of 3x-FLAG tagged S was performed with 1:400 monoclonal ANTI-FLAG® M2 antibody [F3165] produced in mouse (Sigma). 1:50,000 Alexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG [115-625-174] (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2021Quote: ... Lyophilized cytokines (TNF-α, IL-1α, C1q) were obtained from Sigma-Aldrich, resuspended in molecular biology grade water to make stock solutions ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and TNF-α were measured simultaneously using the multiplexed kit “Mouse High Sensitivity T Cell Magnetic Bead Panel” (MHSTCMAG-70K, MILLIPLEX, EMD Millipore Corp., Billerica, MA, USA). The assay was performed according to the manufacturer’s instructions with an overnight incubation step (17 hours ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
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bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Biophysics 2021Quote: ... GST tagged was bought from Sigma-Aldrich (SRP5172). G-actin and biotinylated actin were bought from Cytoskeleton ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
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bioRxiv - Immunology 2022Quote: ... and TNF using a fluorometric bead-based array Multiplex kit (Millipore; Billerica, MA) and a Luminex Multiplex Instrument (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Recombinant Fetuin-A (Sigma; SRP6217) was diluted in 0.9% saline vehicle solution ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant PAK1 (Millipore, 14-927) and agitated at 30°C for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant bacterial sphingomyelinase (S7651, Sigma); 2-bromopalmitate (320-76562 ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant BG4 (recBG4) (Millipore Merck) was added as control.
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Genetics 2019Quote: ... 15 U recombinant DNase1 (Sigma) and 10 U DNA polymerase I (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant DNAse I (Sigma) in MACS buffer (PBS/2mM EDTA/ 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: Recombinant apoE2 (Millipore-Sigma, SRP4760), apoE3 (Millipore-Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 10nM recombinant human gastrin (Sigma), 50ng/mL EGF (Peprotech) ...
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bioRxiv - Physiology 2022Quote: Lyophilised recombinant Aβ42 (#AG912, Millipore) and scrambled control peptide (ScrAβ42 ...