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Citations for Millipore Sigma :
101 - 150 of 3790 citations for Human MOG His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Immunology 2022Quote: ... All cells were incubated for 72 hours with Myelin Oligodendrocyte Protein (MOG) (2.5ug/ml; Sigma-Aldrich, M4939), with or without rIL-12 (25ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin, Millipore Sigma #5893682001) at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Microbiology 2020Quote: ... 7.5 μM mVP40 proteins or His-tag alone were incubated with 50 μg/ml Ni-NTA-Atto 550 dye (Millipore Sigma, Burlington, MA) in a final volume of 500 μl ...
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bioRxiv - Systems Biology 2021Quote: ... The N-terminal His-tag was digested at room temperature overnight using up to 12 NIH Units per mL of bovine thrombin (Sigma-Aldrich T4648-10KU). Proteins were then purified using size exclusion chromatography (HiLoad 26/600 Superdex 200 pg column from GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... in the same buffer containing 1/5000 of primary antibody raised against poly-His tag directly coupled with peroxidase (Sigma, catalog no. A7058). After washing 3x by PBS 1x ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... mice were administered murine MOG 35-55 (Johns Hopkins Peptide Synthesis Core Facility, Baltimore) in incomplete Freund’s adjuvant (Sigma-Aldrich) supplemented with heat-killed mycobacterium tuberculosis (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: Active EAE was induced in female mice by subcutaneously injection of 400 μg of purified recombinant MOG (N1-125) in complete Freund’s adjuvant (freshly made by adding 10 mg/mL Mycobacterium tuberculosis H37 Ra, Sigma-Aldrich). Then ...
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bioRxiv - Neuroscience 2024Quote: ... using a subcutaneous injection of 200mg of MOG peptide (Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35 to 55; from Charité) emulsified in complete Freund’s adjuvant (from Sigma-Aldrich) that contained 200mg of Mycobacterium tuberculosis H37RA (from Difco) ...
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bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-tag (Sigma F3165), Actin (Sigma A2066) ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (M2, Sigma), TOP2A (M042-3 ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tag (Sigma F3165), Tubulin (Sigma T6557) ...
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bioRxiv - Immunology 2019Quote: The sequence-encoding residues Val77–Leu233 of human TNFα was cloned and fused to the N-terminal 6xHis tag in pET-28a+ vector (Novagen) and transformed into Escherichia coli Rosetta (DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Removal of the GST-tag from human and zebrafish Vps29 was performed using on-column cleavage with thrombin (Sigma-Aldrich) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: The C19orf43.WT plasmid is comprised of an N-terminal 3x Flag tag followed by human full length C19orf43 cloned into the p3XFlag-CMV-10 vector (Sigma). C19orf43.R7G was obtained by mutating the arginine at position 7 to glycine and C19orf43.R106G was obtained by mutating the arginine at position 106 to glycine ...
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bioRxiv - Neuroscience 2023Quote: The pCMV20-Igκ_SP-FLAG vectors used for cloning were constructed by inserting a human Igκ signal peptide (SP) sequence in front of the FLAG tag of the pCMV20 plasmid (Sigma). Full-length (36-713 ...
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...