Labshake search
Citations for Millipore Sigma :
101 - 150 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... 17α-hydroxyprogesterone [H-105-1ML] and 11-deoxycortisol [D-061-1ML] were purchased from Sigma-Aldrich. LC-MS grade water [115333] and methanol [106035] ...
-
bioRxiv - Cell Biology 2020Quote: ... pH 4.5 buffer with 200 μM Ala-Ala-Phe 7-amido-4-methylcoumarin (AAF-AMC, Sigma), for 3 hours at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: Wild-type yeast cells were cultured in SM media supplemented either with 12C-glucose (12C-glu; Sigma #G8270) or 13C-glucose (13C-glu ...
-
bioRxiv - Developmental Biology 2024Quote: ... The sperm suspension was capacitated for 1 h in 200 μl HTF medium (Millipore, MR-070-D). Subsequently ...
-
bioRxiv - Microbiology 2022Quote: Asp-pNA was synthesized from protected Fmoc-Asp(Ot-Bu)-OH (Sigma) by coupling with 4-niroaniline (pNA ...
-
bioRxiv - Neuroscience 2022Quote: ... In vitro recombination was achieved using OH-TAM (H7904, Sigma-Aldrich, USA) at a final concentration of 1 µM to 25 cm2 glia culture flasks at least 3 days before harvesting microglia ...
-
bioRxiv - Genetics 2023Quote: ... Medium containing vehicle (ethanol) or 500 nM 4-OH-tamoxifen (Sigma, H7904) was added after BME polymerized ...
-
The ATP-dependent protease ClpYQ degrades cell division proteins DivIVA and Mbl in Bacillus subtilisbioRxiv - Biochemistry 2024Quote: ... and ClpYQ activity was confirmed through hydrolysis of the fluorogenic Cbz-Gly-Gly-Leu-AMC (Millipore Sigma) substrate.
-
bioRxiv - Biochemistry 2023Quote: ... 10 μL of this dilution was then added to 90 μL of buffered D2O (prepared 5 mL with 4.5 mL of D2O and 0.5 mL of 10x protein dilution buffer and 0.2 μg/mL of peptide standard Glu-1-Fibrino peptide (CAS: 103213-49-6, Sigma)) to initiate deuteration at 22 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... Lyophilized PEG-OH (5 g) and 4-Dimethylaminopyridine (DMAP, 0.002 mol; Sigma-Aldrich) were dissolved in anhydrous dichloromethane (DCM ...
-
bioRxiv - Biophysics 2021Quote: ... α-Chymotrypsin, AMC and ANS solutions were prepared in phosphate buffer (10mM, pH 7.0) using water from Millipore system.
-
bioRxiv - Microbiology 2020Quote: ... gentamicin (GEN), and amoxicillin trihydrate:potassium clavulanate (4:1, AMC) were solubilised in molecular grade water (all Sigma, UK), while chloramphenicol (CHL ...
-
bioRxiv - Microbiology 2023Quote: The silanized capillaries and coverslips were modified with AMC-2504 or α-mannose-PEG3-azide(>95%, Sigma-Aldrich) or Azide-fluor 488 (>90% ...
-
bioRxiv - Microbiology 2021Quote: ... 1X L-Glu) and 50% conditioned medium (L-WRN-cell supernatant) supplemented with 10 µM of ROCK inhibitor Y27632 (Sigma) and 10 µM TGF-β inhibitor SB431542 (Millipore ...
-
bioRxiv - Biochemistry 2021Quote: ... The M-class Acquity UPLC with HDX technology coupled to a Waters Synapt G2-Si HDMSE mass spectrometer was used for analysis and calibrated with direct infusion of a solution of glu-fibrinopeptide (Sigma) at 200 fmol/uL prior to data collection ...
-
bioRxiv - Neuroscience 2020Quote: ... muscles were perfused (60-80 mL/h) with heated (28 ± 1 °C) Ringer REES containing 2.3 μg/mL of D-tubocurarine (Sigma) to prevent muscle contraction during nerve stimulation ...
-
bioRxiv - Neuroscience 2021Quote: ... and at DIV10 we treated hippocampal neurons with 1 µM 4-OH-tamoxifen (Sigma) to induce partial Ift88 deletion ...
-
bioRxiv - Plant Biology 2020Quote: ... (±)-3-Hydroxydecanoic acid (3-OH-C10:0) and chitin were obtained from Sigma-Aldrich. All elicitors were dissolved in deionized MilliQ sterile water at the respective stock concentration of 1mM for flg22Pa ...
-
bioRxiv - Neuroscience 2023Quote: ... We also used (±) 8-hydroxy-2- (dipropylamino)tetralin hydrobromide (8-OH-DPAT; 30nM, SIGMA) and Apamin (20nM ...
-
bioRxiv - Cell Biology 2023Quote: RasV12 was activated in inducible lines by addition of 100nM 4-OH-tamoxifen (Sigma). The following small molecule inhibitors were used in this study ...
-
bioRxiv - Cell Biology 2022Quote: ... Doxycycline (Dox, 1 μg/mL) or 4-OH-Tam (1 μM, Sigma-Aldrich, H7904) were added to the medium to induce the expression of TetO-driven transcription or Ngn3ER activation.
-
bioRxiv - Cancer Biology 2024Quote: ... and treated with either (i) 1α,25(OH)2D3 (1µg/kg, H0891ML, Sigma-Aldrich) or (ii ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.001% SDS to the volume of 90 µl per measurement and supplemented with 10 µl of 0.5mM proteasome substrates in the assay buffer: Substrate III (Suc-LLVY-AMC, chymotrypsin-like activity, Millipore), Substrate IV (Z-ARR-AMC ...
-
bioRxiv - Cell Biology 2021Quote: ... Fifteen µg of cell lysate from each hESC line was incubated in 150 µl acetate buffer (pH 4.0) containing a final concentration of 62.5 µM Ala-Ala-Phe-7-amido-4-methylcoumarin (AAF-AMC) (Sigma #A3401) for 20 h at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: TPP1 enzyme activity was measured fluorometrically based on fluorescence of the chromophore 7-amino-4-methylcoumarin (AMC) (Sigma #A9891) as described previously (Lojewski et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... and 50 mM L-Arg and L-Glu by overnight dialysis then concentrated using a 10 kDa Centriprep concentrator (Millipore, UFC901024). The concentrated protein was passed through a pre-equilibrated size exclusion column (Cytiva Custom Superdex TM 75 Increase HiScale TM 16/40 ...
-
bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
-
bioRxiv - Microbiology 2021Quote: ... Ring stage synchronisation (∼0−18 h p.i.) was achieved by a single treatment of infected RBCs with 5% (w/v) D-sorbitol (Sigma-Aldrich), unless otherwise indicated.
-
Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... 10 HEPES adjusted to pH 7.4 with TEA-OH) supplemented with 10 uM Forskolin (Sigma) and 100 uM IBMX (Sigma ...
-
bioRxiv - Developmental Biology 2021Quote: ... either 4 mg of Tamoxifen or 1 mg of 4-OH Tamoxifen (Sigma-Aldrich, H6278) was delivered on day e9.5 or e10.5 (Fig ...
-
TRPA1 block protects against loss of white matter function during ischaemia in the mouse optic nervebioRxiv - Neuroscience 2021Quote: ... and 0.05 Alexa Fluor 594 pH set to 7.15 with K-OH (all from Sigma). For Ca2+ imaging experiments ...
-
bioRxiv - Cancer Biology 2023Quote: ... Neural crest culture medium was supplemented with 200 nM 4-OH-tamoxifen (Sigma no. H7904) in routine culture to ensure nuclear localization of c-MycERT and JoMa1 cell proliferation ...
-
bioRxiv - Cell Biology 2023Quote: ... fibers were cultured for 72 hours in medium containing 4-hydroxytamoxifen (4-OH, 0.4µM, Sigma) or methanol (MeOH ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... we tested the hypothesis that (R)-(+)-8-OH-DPAT (Sigma-Aldrich, product Nr: H140-5MG), a 5-HT1A receptor agonist known to inhibit female sexual behavior (Snoeren et al ...
-
bioRxiv - Cell Biology 2021Quote: ... 2% D-glucose (D; Sigma). To maintain selection of plasmids ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 90 µl of clear supernatant from each sample was transferred into each well of a 96-well black plate and proteasome activity was measured using Suc-LLVY-AMC substrate (Millipore) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2020Quote: ... Remaining protease activity was then detected using a final concentration of 40 μM of Z-FR-AMC (Sigma-Aldrich, C9521). Fluorescent activity was recorded as outlined above ...
-
bioRxiv - Microbiology 2020Quote: ... D-tryptophan (D-trp) D- cycloserine were from Sigma-Aldrich. Individual stock solutions of 100 mM of DAs in Phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... coli BL21 transformed with recombinant pGEX-2T plasmids were diluted 1:10 with fresh medium and grown for 1 h at 37°C before inducing with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma). After another 4 h incubation at 37°C ...
-
bioRxiv - Plant Biology 2023Quote: ... Expression of the recombinant proteins was induced by 1 mM isopropyl β-D-thiogalactopyranoside at 37 °;C for 5 h in host Escherichia coli strain Rosetta (DE3) pLysS cells (Novagen). After induction ...
-
bioRxiv - Biochemistry 2024Quote: ... after which periplasmic expression of nanobodies was induced for another 4 h by addition of 1 mM isopropyl-b-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) to the culture medium ...
-
bioRxiv - Cancer Biology 2024Quote: ... MCF10A iCas9 p53KO cells were transfected with siRNA and after 66 h treated with 150 μM DRB (5,6-dichloro-1- beta-D-ribofuranosylben- zimidazole, Sigma, D1916-10MG) for 6 h ...
-
bioRxiv - Biochemistry 2021Quote: ... corresponding to less than 20% substrate cleavage as measured by comparing fluorescence of the negative control to free AMC (Millipore, 257370), fluorescence was read ...
-
bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
-
bioRxiv - Plant Biology 2024Quote: ... The purified proteasome tested active using succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin (Suc-LLVY-AMC) (Sigma-Aldrich, S6510) was used for further assay55 ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 μL of soluble fraction was incubated with 50 μL of cathepsin B reaction buffer (50 mM sodium acetate, 4 mM EDTA, 8 mM DTT, 50 μM Z-RR-AMC (Sigma, C5429), pH 6 ...
-
bioRxiv - Cell Biology 2020Quote: ... or 2% D-glucose (D; Sigma) or in Synthetic Complete (SC ...
-
bioRxiv - Cell Biology 2020Quote: ... Actinomycin D (Act. D, Sigma Aldrich) was diluted in DMSO to 5 mM and used at 40 nM ...
-
bioRxiv - Molecular Biology 2020Quote: ... Actinomycin D (Act D) (Sigma #A9415) was used in the final concentration of 10 μg/mL in OSC growth medium for 1 h at 25°C.
-
bioRxiv - Neuroscience 2020Quote: ... astrocyte SREBP2 ablation was induced by addition of 100 nM 4-OH tamoxifen (Sigma-Aldrich H7904) to culture media for 48 hours ...