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Citations for Millipore Sigma :
101 - 150 of 10000+ citations for FAS Associated Death Domain Protein FADD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated overnight at 4°C in the following primary antibodies: Fas (EMD Millipore 05-351, 1:500), Phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Microbiology 2021Quote: ... 10% B solvent A = 0.1% formic acid (FA, Sigma) in water (Thermo-Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... A solution of 4.4% essentially FA-free BSA (Sigma) in PBS was warmed to 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... For FA: 25-200 µM palmitate (Sigma Aldrich, #P0500) bound to fatty-acid free BSA (5g/50mL in 10% PBS ...
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bioRxiv - Microbiology 2020Quote: ... Acetone precipitation/formic acid (FA; Sigma-Aldrich, MO, USA) solubilisation was added as a further sample clean-up procedure ...
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bioRxiv - Molecular Biology 2022Quote: ... and the indicated concentration of FA (Sigma-Aldrich, F8758). The reagents used in cell culture experiments are as follows ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μl 8% formaldehyde (FA, 36.5-38%, F8775, Sigma)/8% acrylamide (AA ...
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bioRxiv - Molecular Biology 2020Quote: 10 μL samples containing 10 μM protein (TASOR variants or PARP1 catalytic domain) in the presence or absence of benzamide (Sigma) were loaded into glass capillaries (Nanotemper ...
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Molecular Biology 2024Quote: ... For shRNA-mediated knockdown, all TRC pLKO.1 vectors (targeting the YTH domain-containing proteins, THOC1, and THOC2) were purchased from Sigma. The TRIPZ doxycycline (Dox)-inducible lentiviral YTHDC1 shRNA vector is purchased from Dharmacon.
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bioRxiv - Microbiology 2023Quote: ... the purified proteins and domains were pooled and concentrated to 0.2–1 ml in PBS buffer by Amicon Ultra-15 (Millipore, USA). A BCA Protein Assay Kit (Beytotime Bio Co Ltd. ...
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bioRxiv - Microbiology 2020Quote: ... Bound domain C was then detected using an HRP-conjugated anti-FLAG antibody (Sigma-Aldrich, St. Louis, MO) and Ultra-TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated with the primary antibody (anti-Htt raised against the Proline-rich domain (PRD) (MAB5492, Millipore) at a dilution of 1/500 in PBST for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Resulting primary cells were first cultured in the selective media to proliferate in the presence of the Rho-associated protein kinase (ROCK) inhibitor (Y-27632, 10 µg/ml, Sigma-Aldrich, Poland) (95) ...
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bioRxiv - Cell Biology 2019Quote: ... N-SH2 domain (ABS233) was obtained from Millipore Corp ...
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bioRxiv - Cell Biology 2024Quote: ... FH2 domain inhibitor SMIFH2 (2µM; Sigma-Aldrich #344092); Rac activation ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL assays were performed after secondary antibody staining by using the In Situ Cell Death Detection Kit TMR red (Millipore #12156792910). Embryos were incubated for 4 hours at room temperature (RT ...
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bioRxiv - Microbiology 2021Quote: ... All PEG and associated chemical formulations were obtained from Sigma and purchased as chemical compounds ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... and associated wash buffers (Sigma-Aldrich, Cat. No. DUO82047-4L) and PLA probes according to manufacturer protocol with modifications to blocking and primary antibody incubation steps ...
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bioRxiv - Bioengineering 2022Quote: ... The Roche In Situ Cell Death Detection Kit (Lot:41569800, Sigma) was used to visualize apoptotic cells in frozen sections of cardiac organoids on the basis of the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... TUNEL (In Situ Cell Death Detection Kit, TMR Red, Sigma/Roche); anti-Kmg (Fuller lab).
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Biophysics 2021Quote: ... and the flow-through fractions containing the MgtE TM domain protein were concentrated using an Amicon Ultra 50 K filter (Merck Millipore, USA). After concentration ...
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bioRxiv - Cell Biology 2024Quote: ... A pair of gRNAs with minimum off-target and maximum on-target activity were designed and selected to specifically target sequences that encode essential protein domains of the Bend2 gene using CRISPR DESIGN TOOLS (Millipore Sigma) (gRNA1-antisense ...
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bioRxiv - Immunology 2021Quote: ... or CTB-S5 in complete Freund’s adjuvant (FA, Sigma, USA) on day 0 and in incomplete FA on the 7th and 14th day ...
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bioRxiv - Microbiology 2021Quote: ... 3% B where solvent A = 0.1% formic acid (FA, Sigma) in water (Thermo-Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Ser-FA (BHI containing eFA+10% newborn calf serum; Sigma) and SerFA-Tric (SerFA+Triclosan 0.5 μg/ml) ...
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bioRxiv - Molecular Biology 2020Quote: ... Formic acid (FA) was obtained from Sigma-Aldrich (Oakville, ON). Stable isotope labeled peptides (SpikeTides_L and SpikeTide_TQL ...
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bioRxiv - Immunology 2021Quote: ... UreB or BSA in complete Freund’s adjuvant (FA, Sigma, USA) on day 0 and in incomplete FA on days 7 and 14 ...
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bioRxiv - Cell Biology 2022Quote: ... Treatment with the pharmacological FAS inhibitor cerulenin (#C2389, Sigma-Aldrich) was carried out by adding the drug directly to the cell cultures at the concentration and times indicated in the figure legends.
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bioRxiv - Neuroscience 2022Quote: ... or 4% FA + 20% acrylamide (AAm; Sigma; catalog no. A9099) (all in 1× PBS ...
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bioRxiv - Biochemistry 2024Quote: ... After adding tricosanoic acid (FA 23:0) (Sigma-Aldrich, USA) as an internal standard ...
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bioRxiv - Genomics 2023Quote: ... The peptides were dissolvedin 0.1% formic acid (FA; Sigma Aldrich), and approximately 1μg of peptides was separated on an Acclaim PepMapTM C18 column (75 μm I.D ...
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bioRxiv - Neuroscience 2020Quote: ... Dendrites were identified using a staining against the microtubule-associated-protein-2 with Rabbit-anti-MAP2 PAB (Milipore Sigma, cat. AB5622, 1:1000) and GAR-STAR488 SAB (Abberior ...
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bioRxiv - Pathology 2021Quote: ... The DNP signal associated with RyR was determined using a specific anti-DNP antibody according to the manufacturer’s instructions (Millipore, Billerica, MA). All immunoblots were developed and quantified using an Odyssey system (LI-COR Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-Gal4 activation domain (1:1000, Millipore Sigma). Alexa Fluor secondary antibodies were used (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... MBP domain was cleaved with TEV protease (Sigma Aldrich). These proteins were flash-frozen with 40% glycerol in a solution containing 50 mM Sodium Phosphate (pH 7.2) ...
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bioRxiv - Immunology 2021Quote: ... For RBD inoculations eight-week-old male BALB/c mice were immunized with 7.5 μg of the receptor binding domain of Spike protein (kindly gifted by Andrea Gamarnik, Argentina) in 75 μL Complete Freund’s Adjuvant (CFA, Sigma, St. Louis, MO) via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Bioengineering 2020Quote: ... 3) Rho-associated kinase (ROCK) inhibitor Y27632 (10 μM; Sigma-Aldrich), 4 ...
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bioRxiv - Cell Biology 2021Quote: ... the Rho-associated kinase (ROCK) inhibitor Y27632 (10 μM; Sigma-Aldrich), the actin de-polymerizer latrunculin B (10 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... cell proliferation and cell death assays were anesthetized using 1x benzocaine (Sigma) and fixed overnight in 4% paraformaldehyde (PFA ...
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bioRxiv - Developmental Biology 2019Quote: ... we used the In Situ Cell Death Detection Kit (Sigma-Aldrich, 11684795910), following the manufacturer’s protocol (ver ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were treated with in situ Cell Death Detection Kit (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Percentage cell death was measured using Cell Cytotoxicity Detection kit (LDH; Sigma) as per manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: ... using a commercially available In Situ Cell Death Detection kit (Sigma-Aldrich), according to the manufacturer’s protocol (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... we added polyclonal antibodies against the extracellular domain of TrkB (1:50 rabbit anti-TrkB, Millipore, cat no. AB9872, RRID:AB_2236301) together with 20 ng/mL BDNF for 2.5 hours ...
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bioRxiv - Biophysics 2020Quote: ... and 10 μg/ml anti-E-cadherin blocking antibody that targeted the extracellular domain of E-cadherin (Gumbiner, Stevenson and Grimaldi, 1988) (uvomorulin, monoclonal anti-E-cadherin antibody, Sigma); after incubation ...
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bioRxiv - Cell Biology 2020Quote: ... FA solution (12 μL, 37 wt. % in water, Sigma-Aldrich, #F8775) and HCl (0.5 ml ...
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bioRxiv - Cell Biology 2020Quote: ... FA and/or L-buthionine-sulfoximine (L-BSO, Sigma-Aldrich, #B2515) and/or antioxidants were added to a final volume of 200 μl per well ...