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Citations for Millipore Sigma :
101 - 150 of 9923 citations for 7 Oxa 12 thia 3 aza spiro 5.6 dodecane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... and the pellets were resuspended with room temperature HPLC-grade 3:7 methanol:acetonitrile (Sigma Aldrich) and centrifuged at 13,000 rpm for 5 minutes ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... and 3 ug/ml 5-fluoro-2’-deoxyuridine + 7 ug/ml uridine (FRD+U; Sigma) added ...
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bioRxiv - Molecular Biology 2023Quote: ... 4-triazol-3-yl)thio)acetamide) (CAS 525582-84-7) was purchased from Sigma-Aldrich. The stock solution was 110 mM in DMSO and subsequently diluted into appropriate buffer solution.
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine ...
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bioRxiv - Immunology 2023Quote: The following small compound inhibitors and reagents were used: caspase-3/7 inhibitor I (Sigma), CRID3 (MCC-950 ...
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bioRxiv - Cell Biology 2023Quote: ... and CellEvent™ Caspase-3/7 green ReadyProbes™ Reagent (2 drops/ml, Sigma, R37111) for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... 7-hydroxycoumarin (7-HC, Sigma-Aldrich), generated from coumarin was quantified via fluorescence detection (excitation/emission ...
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bioRxiv - Neuroscience 2020Quote: ... to 30 µL CSF (pooled from 1 – 3 animals) and placing in 3 mm NMR tubes (Wilmad®, 7 inch; Sigma-Aldrich). Serum samples (samples pooled correspondingly to the CSF samples ...
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bioRxiv - Plant Biology 2020Quote: ... The cultures were then pelleted and resuspended in equal volumes of induction medium (10 mM MgCl2, 10 mM MES, pH 5.6) also containing 100 μM acetosyringone (Sigma) and maintained at room temperature for 4 h ...
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bioRxiv - Plant Biology 2020Quote: ... clv2 and fin mutants was conducted using half strength LANS containing 2.5 mM NaH2PO4 and 5.6 mM KNO3 (Hewitt 1966) solidified with 5g phytagel/L (Sigma, USA). In subsequent experiments that aimed to examine the tomato root morphology response under different N conditions ...
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bioRxiv - Microbiology 2020Quote: ... The resulting purified peptidoglycan was digested overnight at 37°C in 12.5 mM sodium phosphate buffer (pH 5.6) supplemented with 100 UI of mutanolysin from Streptomyces globisporus ATCC 21553 (Sigma). The reaction was stopped by heat inactivation of the enzyme and insoluble material was removed by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... we are able to specifically label Sec residues and block Cys residues in both cell lines and tissues42,52.Cell or tissue were lysed in MES lysis buffer pH 5.6 (50mM MES (Sigma Aldrich), 150mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stimulated with 50 μL of 10−7 M phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) or N-formyl-methionyl-leucyl-phenylalanine (fMLP ...
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bioRxiv - Molecular Biology 2019Quote: ... 5% CO2 and 3% O2 in Dulbecco’s Modified Eagle’s Medium DMEM/F-12 (Sigma), supplemented with 10% Fetal Calf Serum (FCS ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2024Quote: ... 7-aminoactinomycin D (7-AAD, Sigma-Aldrich) was added to stain dead cells ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed with PBS twice and treated with 10 μM of 5-aza (Sigma, St. Louis, USA) in DMEM-F2 medium (DMEM-F2 ...
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bioRxiv - Cell Biology 2019Quote: ... Cultures were then treated with 50 ng/mL LMB dissolved in 7:3 MeOH:H2O solution (Sigma) for 45 minutes alongside a control of the equivalent volume of MeOH before imaging.
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bioRxiv - Neuroscience 2023Quote: ... NBQX (118876-58-7) and Spermine (7144-3) were purchased from Sigma-Aldrich (Saint-Louis, US). MNI-glutamate (HB0423 ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg/mL 5-fluoro-2′-deoxyuridine + 7 μg/mL uridine (FRDU, F0503, Sigma-Aldrich). Cultured cells were then kept at 37°C and 5% CO2 in an incubator with culture media changes at 48 hours with supplemented media and NGF and FRDU until further experimentation.
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens GV3101 strains harboring expression constructs of interest were pre-incubated at room temperature (ca. 22°C) in induction media (10 mM MES pH 5.6, 10 mM MgCl2, 150 mM acetosyringone [Sigma-Aldrich]) for 2 h before hand-infiltration into 5-week-old N ...
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bioRxiv - Developmental Biology 2024Quote: ... along with Cas9 protein (0.3 mg/ml), gRNA (5.6 µM crRNA and tracerRNA, synthesized by IDT Integrated DNA Technologies) and 0.2 µM KCl (Sigma, P9333-500G) into one-cell stage embryos ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 2014) and to standards injected with the same temperature program (benzyl alcohol, acetophenone, nonanal, dodecane, decanal, all from Sigma Aldrich, Saint- Louis, MO, USA).
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bioRxiv - Microbiology 2019Quote: ... The monocytes cells were differentiated into macrophages by addition of 10−7 M phorbol 12-myristate 13 acetate (PMA) (Sigma) for 48 hours before the infection ...
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bioRxiv - Neuroscience 2020Quote: ... 50 −100 μg of protein were loaded on a 7%-12% polyacrylamide gel and then transferred on a methanol activated PVDF membrane (Millipore). Blot membranes were blocked for one hr with 5% milk or 5% BSA in TBS with 0.1% Tween 20 (TBST) ...
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bioRxiv - Molecular Biology 2022Quote: ... For Huh7-dCas9 knockdown in 12-well dishes 30 µL of each gRNA was combined with 7 µL TransIT-CRISPR transfection reagent (Sigma) in 363 µL OPTI-MEM (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 100 mg fragments were incubated in a 12-well plate with M199 media ± 7 nM insulin (Actrapid, Novo Nordisk) and 25 nM dexamethasone (D4902, Sigma). After 24 hr incubation ...
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bioRxiv - Systems Biology 2020Quote: BALB/cJ mice were subjected to cutaneous Oxazolone (Oxa) challenge by applying 5% 4-Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in acetone and olive oil topical to the skin as described [74] ...
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bioRxiv - Immunology 2022Quote: Mice were sensitized on the shaved back-skin for two consecutive days with 50 μl of 5% Oxa (4-Ethoxymethylen-2-phenyl-2-oxazolin-5-on, Sigma-Aldrich) diluted in methanol and acetone (1:1 ...
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bioRxiv - Cell Biology 2022Quote: ... Media was changed every 2-3 days for new media: DMEM/F-12 (Sigma-Aldrich) + 10% FBS (Gibco ...
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bioRxiv - Immunology 2019Quote: ... and 7-aminoactinomycin D (7-AAD; Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... cells grown to 60-70% confluency were exposed to a single dose of 2μM 5-aza-2ʹ -deoxycytidine (Sigma-Aldrich) diluted in 1:1 acetic acid/water ...
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bioRxiv - Cancer Biology 2021Quote: HDMB03 cells (3×105 in 6-well plates) were treated with global de-methylating agent 5-AzaC (5 μM) (5-Aza-2-deoxycytidine; Sigma). Following 96 hours of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... we incubated either control THP-1 or SRRM2 knockdown THP-1 cells with different concentrations of CPT or AZA (Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2023Quote: ... extracted from MEFs with the indicated genotype after treatment with 10 μM 5-Aza for 48 hours or after treatment with an equal volume of DMSO (SIGMA). Total RNA was extracted using a Direct-zol Zymogen RNA miniprep kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2023Quote: ... the following doses of chemicals and genotoxic agents were used: 5-Aza-2’-deoxycytidine (5-AzadC; 10 μM, Sigma-Aldrich), 5-Ethynyl-2’-deoxyuridine (EdU ...
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bioRxiv - Biochemistry 2022Quote: ... and concentrated to 13.7 mg·ml-1 (A280=23.36) using a centrifugal filter (Amicon Ultra-0.5, MWCO 3 kDa, Merck Millipore) prior to crystallization ...
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bioRxiv - Biochemistry 2022Quote: ... Either Phosphorylcholine 2mM (Avanti) or a combination of DMPC and DMPG (7:3 molar ratio) (Avanti/Sigma) were resuspended in Chloroform:Methanol (95:5 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and thus 3-[2-(diethylamino)ethyl]-7-hydroxy-4-methylcoumarin (AHMC; Cat. No. 188611, Sigma-Aldrich Corp.) was used instead as previously described for the fluorescence quantification (Donato et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... pH 7 using repetitive washing and centrifugation with an Amicon 3 kDa MWCO centrifugal filter (Merck Millipore). For the synthesis of ditopic A’-A’ CC ligand ...
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bioRxiv - Plant Biology 2023Quote: ... 3-week-old plant leaves were infiltrated with 50 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Millipore-Sigma) in 50 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... 3-week-old plant leaves were infiltrated with 50 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Millipore-Sigma) in 50 mM phosphate buffer (pH 7.4 ...
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Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomatobioRxiv - Plant Biology 2023Quote: ... 1/3 of plants were soil drenched with 5 mM BABA (catalogue number A4420-7, Sigma Aldrich) to provide a final concentration of 0.5 mM in the soil ...
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bioRxiv - Cancer Biology 2023Quote: ... Analysis was done using the caspase-3/7 protocol on the Guava Muse® cell analyzer (Millipore).
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bioRxiv - Plant Biology 2020Quote: ... cells were harvested by centrifugation and resuspended in 10 mM MES (pH 5.6) buffer containing 10 mM MgCl2 and 200 μM acetosyringone (Sigma, Cat. #D134406) at OD600 = 1.0 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the pellets were resuspended in infiltration buffer (10 mM MES, 10 mM MgCl2, pH 5.6) with 100 μM acetosyringone (3,5-dimethoxy-4-hydroxyacetophenone, Sigma-Aldrich, Steinheim, Germany) and slightly shaken at room temperature for 1-3 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were seeded in 15-well IBIDI angiogenesis chambers during passaging and fixed 7-12 days later in 4% paraformaldehyde (PFA) (Sigma-Aldrich) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Neuroscience 2022Quote: ... BrdU injections were done 24h before sacrifice for the analysis at 3 dpKA and 3/7 days post administration of zinc (Cat#83265-250ML-F, Sigma-Aldrich, St Louis, MO, USA). To identify newborn cells at 14 dpKA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... R189A/R206A has been previously described to disrupt the dimer interface of OXA-48 and elute significantly later.41 Separation was performed in 0.1 M phosphate buffer (Sigma-Aldrich, pH 7.0) using a Superdex 200 10/300 GL column with a flow rate of 0.5 mL per min at 4°C ...