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Citations for Millipore Sigma :
1401 - 1450 of 7256 citations for West Nile Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... bead-bound proteins were eluted in Laemmli buffer (Sigma) and resolved on a 4–20% SDS-polyacrylamide gel (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 30 μl of protein A agarose beads (Sigma). Samples were subjected to SDS-PAGE and immunoblot analysis as described (16,20,23,82,86–87) ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted proteins were precipitated with trichloroacetic acid (Sigma) and subject to MS analyses as described previously 13.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore, IPVH85R) at 120V for 65 min at 4C in 1x tris-glycine-methanol transfer buffer [25 mM tris base ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SR proteins (Millipore, cat#MABE126, 1:2,000 dilution); anti-phosphoepitope SR proteins clone1H4 (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse antiglial fibrillary acidic protein (GFAP) (Millipore, 1:500), Beta-actin (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... and proteins were eluted in 2X Laemmli (Sigma-Aldrich) or deglycosylated by an 1h treatment with PNGase F (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
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bioRxiv - Neuroscience 2022Quote: ... Pelleted proteins were resuspended in 0.1 M NaOH (Sigma) and assayed for protein quantification using the Bradford assay (Biorad).
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, Millipore-Sigma, 1:400), and exceeded 90%.
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bioRxiv - Neuroscience 2022Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) final concentration at 95°C for 10 minutes and alkylated with iodoacetamide (Pierce,40 mM final 30 minutes) ...
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bioRxiv - Neuroscience 2022Quote: ... 30ul of equilibrated Protein G – agarose beads (Sigma 15920010) were added to the lysates and incubated further for 3 hours.
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bioRxiv - Neuroscience 2022Quote: ... and proteins were extracted in RIPA buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitors (Pierce Protease and Phosphatase Inhibitor Mini Tablets ...
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bioRxiv - Neuroscience 2022Quote: ... myelin basic protein (MBP; 1:200; MAB386; Milipore Sigma), Synaptophysin (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein synthesis inhibitor cycloheximide was purchased from Sigma-Aldrich. USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A ...
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bioRxiv - Cell Biology 2022Quote: ... the protein was concentrated using Amicon Ultracel-30K (Millipore) centrifuge filters and subsequently applied to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Systems Biology 2022Quote: ... Protein was digested with sequencing grade trypsin (Sigma; #T6567) and sequencing grade LysC (Wako ...
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bioRxiv - Systems Biology 2022Quote: ... The protein was transferred to PVDF membranes (Millipore; #IPFL00010) in NuPAGE Transfer Buffer at 20 Vfor 1 h at room temperature and blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBST ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred to methanol activated PVDF (Millipore) membranes (350 mA for 60 minutes) ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Genomics 2022Quote: ... bound to Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Co-purification was investigated by western blot with an anti-GFP antibody (Roche #11814460001 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were transferred to PVDF-FL membranes (Millipore) as previously published (Michele et al. ...
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bioRxiv - Biophysics 2022Quote: All Proteins and fluorophores were procured from Sigma-Aldrich and concentration was measured using Shimazdu uv-1900 spectrophotometer ...
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bioRxiv - Immunology 2022Quote: ... The proteins were transferred onto PVDF membranes (Millipore, USA), then blocked with 5 % non-fat dried milk for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2023Quote: ... levels were normalized to total protein levels (Sigma-Aldrich, #E3138 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto a Immobilon PVDF membrane (Millipore), which was then blocked in 4% milk in tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Samples were precleared with Protein A agarose beads (Millipore), immunoprecipitated with anti-REV-ERBα (NRIDI ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37ÅãC and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 60µL of Protein A beads (EMD Millipore, 16-156) were washed in IP lysis buffer then blocked for 1hour in 1%BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with ReadyBlue Protein Gel Stain (Sigma RSB-1L) overnight for Coomassie staining ...
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bioRxiv - Cell Biology 2023Quote: Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were dimethylated by adding 30 mM formaldehyde (Sigma) and 30 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Biochemistry 2023Quote: ... After a preclearing step with Protein G Sepharose (Sigma), equal amounts of proteins were then incubated with anti-Flag Beads (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...
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bioRxiv - Immunology 2023Quote: Protein samples were boiled in Laemmli buffer (Sigma-Aldrich) at 95°C for 5 min and separated on a 12% Bis-Tris gel (NuPAGE ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was confirmed using Ponceau S (Sigma-Aldrich), and blots were cut into two at relevant molecular weights as needed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein constructs were expressed in BL21 (DE3) cells (Novagen), in 2xYT media ...
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was determined by Bradford reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Protein was then transferred to a PVDF membrane (Millipore) overnight with NuPAGE transfer buffer ThermoFisher ...
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bioRxiv - Pathology 2023Quote: ... and separated proteins were transferred on PVDF membrane (Millipore). After blocking aspecific signal ...
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were quantified by Bradford reagent (Sigma) and purity was analysed by SDS-PAGE ...
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bioRxiv - Pathology 2023Quote: ... and separated proteins were transferred on PVDF membrane (Millipore). After blocking aspecific signal ...