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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Dengue virus was added at a MOI=1 in a minimum volume of Dubelcco’s PBS with CaCl2 and antibiotics (SIGMA-Aldrich). After 2 hours ...
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bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cells were resuspended at a concentration of 106 cells/mL in virus-containing medium supplemented with 10 μg/mL polybrene (Sigma), divided into 6-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-CAMSAP2 was eluted in 50 μl TEV cleavage buffer containing 0.5 μg of glutathione S-transferase-6x-histidine Tobacco etch virus protease site (Sigma-Aldrich) for 2 hr at 4 °C.
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bioRxiv - Immunology 2022Quote: ... hTert-BJ1 or U937 were infected with 2 ml of fresh virus in presence of 8µg/ml Protamine (Millipore Sigma). To generate transduced MDDCs ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and the virus was resuspended in ultrapure water containing 0.005% Bovine serum albumin (Sigma-Aldrich, USA). Purified and filtrated SARS-CoV-2 pseudovirus was firstly fixed in 2% glutaraldehyde (Leagene ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were infected with either of the sgPLXNB2 clones or the control sgRNA with Cas9-GFP virus (Sigma-Aldrich #0030) at 10 IFU/cell in Opti-MEM (Thermo Fisher Scientific #31985070 ...
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bioRxiv - Cancer Biology 2023Quote: ... and NHA cells were transduced with equal titers of concentrated virus in complete growth media supplemented with 1 μg/ml polybrene (Sigma) for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Neuroscience 2023Quote: ... The media containing lentivirus was collected and virus particles were concentrated 20- fold using Amicon Ultracel-100k (EMD Millipore 901024). For all the experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants containing purified virus were applied onto HeLa cells for 48 h in the presence of 8 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Cancer Biology 2023Quote: ... 786-O cells were cultured with virus containing media and 4µg/mL polybrene (Sigma Aldrich, Cat. No. TR-1003-G) for 24 hours ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... 600,000 cells were seeded into each well of a 6-well plate and one week later were infected with up to 100ul virus per well (and 8 ug/ml polybrene (TR-1003-G, Sigma)) overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral titres were calculated by infecting PMA-treated THP-1 cells (2×105 cells/ml) or U87 cells (105 cells/ml) with dilutions of virus in the presence of polybrene (8 μg/ml, Sigma) for 48 h and enumerating GFP-positive cells by flow cytometry using the FACS Calibur (BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor cell lines and UPCs were infected with the concentrated virus in a culture media containing 8 µg/ml Polybrene (Sigma) and positively transduced cells selected by FACS for the expression of the corresponding fluorescent reporter.
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bioRxiv - Biophysics 2023Quote: ... The cells were then infected in triplicate with VSV-G-pseudotyped GFP-encoding virus in the presence of polybrene (10 µg/ml; Sigma) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... The next day 50 µl of concentrated virus was added together with 5 µg/ml polybrene (Millipore, TR-1003-G) to the culture media ...
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bioRxiv - Immunology 2024Quote: ... Virus was plaque-purified on Vero CCL81 cells in the presence of 25 μg/mL of cytosine arabinoside (Sigma-Aldrich), and plaques in agarose plugs were amplified on Vero CCL81 cells ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
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bioRxiv - Molecular Biology 2020Quote: ... Injection capillaries (PIEZO 8-15-NS, Origio, Charlottesville, USA) were filled with Fluorinert (FC-770, Sigma) for proper Piezo pulse propagation ...
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bioRxiv - Biochemistry 2022Quote: ... HMEC are maintained in 10% FCS/Pen-Strep MCDB media supplemented with 2mM L-Glutamine (Sigma), EGF (2ng/ml ...
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bioRxiv - Genetics 2020Quote: ... supplemented with 10 % (w/v) fetal calf serum (FCS)(PAA) and 2 mM L-glutamine (Sigma) at 37 °C with 5 % CO2 and saturated humidity ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS; PAA, Linz, Austria) and 2 mm glutamine (Sigma Aldrich). Cells were selected for acquired drug resistance over several months via exposure to increasing concentrations of oxaliplatin or BOLD-100/KP1339 followed by drug-free recovery phases ...
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bioRxiv - Cell Biology 2021Quote: ... S2R+ Cells (2 × 105) were seeded in Schneider medium plus 10% FCS (Gibco 21720024, Sigma F9665) in a 24-well plate ...
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bioRxiv - Immunology 2022Quote: ... in PBS and stored in washing buffer (PBS+5%FCS+0.2% Triton X-100 (Sigma-Aldrich)+0.01% Thimerosal (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Proteinase K activity was blocked by the addition of 20% foetal bovine serum (FCS) (F7524, Sigma) followed by rinsing in R16 medium ...
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bioRxiv - Biochemistry 2020Quote: ... ScFv-hFc or IgG were detected using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Titration assays were performed using 384 well microtiter plates (Costar ...
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bioRxiv - Cell Biology 2021Quote: ... University of Utrecht) were maintained in DMEM supplemented with 10% fetal calf serum (FCS; Sigma A7906) and 100 U/ml Pen/100 μg/ml Strep (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... spinal cords were overlaid with 3ml of F12 medium supplemented with 10% FCS (F7524; Sigma-Aldrich), 1% Penicillin/Streptomycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... diluted 1:1 000 with secondary antibody Anti-Mouse IgG (Fc specific) HRP (Sigma-Aldrich #A0168) diluted 1:5 000 ...
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bioRxiv - Developmental Biology 2022Quote: ... They were cultured in FC supplemented with 1 μg ml-1 heparin (Sigma-Aldrich H3149-25KU), and 25 ng ml-1 FGF4 (R&D Systems 7486-F4-025 ...
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bioRxiv - Cancer Biology 2023Quote: ... mice were intraperitoneally injected with 250 mg/kg 5-fluorocytosine (5-FC) (Sigma Aldrich, Cat: F71291G), and after 12 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and resuspended in RPMI 1640/FCS with 50 μM 2-mercaptoethanol (Sigma-Aldrich) at concentration of 106 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... The infected cells were cultured in DMEM with 10% FCS and 2μg/ml of cycloheximide (Sigma) for 24h ...
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bioRxiv - Immunology 2023Quote: ... The cell pellet was resuspended in RPMI medium (Capricorn, RPMI-STA) containing 10 % FCS (Sigma, F7524). Before expansion ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were frozen in DMEM+FCS+Pen/Strep supplemented with 20% FICOL-PM400 (Sigma, F4375-25G) as a cryoprotectant ...
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bioRxiv - Cell Biology 2023Quote: ... FC and DN ARF6 for 24 hours were lysed in RIPA buffer (EMD Millipore, 20-188) containing protease inhibitors ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FCS (Eurobio Scientific, CVFSVF00-01) and containing 1% penicillin-streptomycin (Sigma-Aldrich, P4333). All cells were grown in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in 10% FCS/PBS and fixed either with 1% PFA (Sigma-Aldrich #252549) for ChIP-seq or with 2% PFA for C-HiC for 10 minutes rolling ...
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bioRxiv - Biophysics 2021Quote: PAI-1 (human recombinant, Sigma-Aldrich, Germany)
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bioRxiv - Biophysics 2021Quote: ... PAI-1 (human recombinant, Sigma-Aldrich, Germany), Plasminogen (human plasma purified protein ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human thrombin (1 U/ml, Sigma) was added to the bottom of a 3.0 µm costar polycarbonate transwell membrane insert (Corning ...