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1401 - 1450 of 10000+ citations for Recombinant Mouse Ldlr His tagged since 2019

• BacScan: An Unbiased and Genome-Wide Approach to Identify Bacterial Highly Immunogenic Proteins

  Junhua Dong, et al., bioRxiv - Microbiology 2023

  Quote: ... The recombinant proteins were purified using a Ni-NTA column (Yeasen, Wuhan, China) and concentrated by ultrafiltration (Millipore). Protein concentration was determined by SDS-PAGE using BSA as a standard.
• Reverse engineering of a pathogenic antibody reveals the molecular mechanism of vaccine-induced immune thrombotic thrombocytopenia

  Daniil G. Ivanov, et al., bioRxiv - Immunology 2023

  Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
• Reverse engineering of a pathogenic antibody reveals the molecular mechanism of vaccine-induced immune thrombotic thrombocytopenia

  Daniil G. Ivanov, et al., bioRxiv - Immunology 2023

  Quote: RVT1 association with the recombinant form of human PF4 and UPS-grade protamine (Millipore-Sigma, St. Louis, MO) were characterized using a Synapt G2 HDMS (Waters Corp. ...
• Oxygen levels at the time of activation determine T cell persistence and immunotherapeutic efficacy

  Pedro P. Cunha, et al., bioRxiv - Immunology 2022

  Quote: ... CD8+ T cells were expanded in the presence of 10 U/mL recombinant human IL-2 (11147528001, Sigma). Human CD8+ T cells were purified from donor PBMCs (NHSBT or Karolinska Hospital ...
• Loss of cholesterol in Junctional Epidermolysis Bullosa skin identifies a key role for Laminin-332 in actomyosin mediated cholesterol transport

  Eleri. M. Jones, et al., bioRxiv - Cell Biology 2023

  Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
• The Pex6 N1 domain is required for Pex15 binding and proper assembly with Pex1

  Bashir A. Ali, et al., bioRxiv - Biochemistry 2023

  Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
• Reproducible differentiation of pure ovarian support cells from clinical-grade hiPSCs as a novel infertility treatment

  Bruna Paulsen, et al., bioRxiv - Bioengineering 2024

  Quote: ... Supplemented IVM media consisted of MediCult IVM media (Origio) supplemented with 75 mIU/mL of recombinant FSH (Millipore), 100 mIU/mL recombinant hCG (Sigma) ...
• RV-specific Targeting of Snai1 Rescues Pulmonary Hypertension-induced Right Ventricular Failure by Inhibiting EndMT and Fibrosis via LOXL2 Mediated Mechanism

  Somanshu Banerjee, et al., bioRxiv - Physiology 2024

  Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat #T7039, Sigma)25 for 72 h ...
• RV-specific Targeting of Snai1 Rescues Pulmonary Hypertension-induced Right Ventricular Failure by Inhibiting EndMT and Fibrosis via LOXL2 Mediated Mechanism

  Somanshu Banerjee, et al., bioRxiv - Physiology 2024

  Quote: ... were incubated in hypoxia condition in the hypoxia chamber with TGFβ1 (recombinant human TGFβ1: 15ng/ml, Cat # T7039, Sigma)10 for 72 h ...
• A comparative study of the cryo-EM structures of S. cerevisiae and human anaphase-promoting complex/cyclosome (APC/C)

  Ester Vazquez-Fernandez, et al., bioRxiv - Molecular Biology 2024

  Quote: ... The recombinant APC/C complex was eluted with the wash buffer supplemented with 2.5 mM desthiobiotin (Sigma-Aldrich). Before loading the elution fractions onto a 6 mL Resource Q anion-exchange column (Cytiva) ...
• PxdA interacts with the DipA phosphatase to regulate endosomal hitchhiking of peroxisomes

  John Salogiannis, et al., bioRxiv - Cell Biology 2020

  Quote: ... Mouse anti-HA (Sigma) was used at 1:1000 ...
• The postsynaptic MAGUK scaffold protein MPP2 organises a distinct interactome that incorporates GABA_A receptors at the periphery of excitatory synapses

  Bettina Schmerl, et al., bioRxiv - Neuroscience 2020

  Quote: ... αFlag (mouse, F1804, Sigma), αFlag-HRP (mouse ...
• Arenavirus nucleoprotein localizes to mitochondria

  Francesca Baggio, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse anti-HA (Sigma, H3663 ...
• HIV-2/SIV Vpx antagonises NF-κB activation by targeting p65

  Douglas L. Fink, et al., bioRxiv - Molecular Biology 2021

  Quote: ... mouse-anti-FLAG (Sigma), rabbit-anti-HA (Sigma) ...
• A versatile system to introduce clusters of genomic double-strand breaks in large cell populations

  Thorsten Kolb, et al., bioRxiv - Genomics 2020

  Quote: ... raised in mouse (Millipore), according to the manufacturer’s protocol ...
• The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant specific motif and acts in a partially redundant manner with the tonoplast enzyme

  Upendo Lupanga, et al., bioRxiv - Plant Biology 2020

  Quote: ... anti-mouse IgG (Sigma) for VHA-B and chemiluminescent substrate (Peqlab) ...
• GCNA interacts with Spartan and Topoisomerase II to regulate genome stability

  Gregory M. Davis, et al., bioRxiv - Developmental Biology 2019

  Quote: ... mouse anti-MLH1 (Millipore). Images were collected using a DeltaVision system (Applied Precision ...
• Ral GTPases promote metastasis by controlling biogenesis and organ colonization of exosomes

  S Ghoroghi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... RalB (mouse, 04037; Millipore). The following secondary antibodies were used ...
• Ral GTPases promote metastasis by controlling biogenesis and organ colonization of exosomes

  S Ghoroghi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... α-tubulin (mouse, CP06; Millipore) and Secondary horseradish peroxidase -linked antibodies ...
• Ral GTPases promote metastasis by controlling biogenesis and organ colonization of exosomes

  S Ghoroghi, et al., bioRxiv - Cancer Biology 2020

  Quote: ... RalB (mouse, 04037; Millipore), Glypican 4 (Rabbit ...
• Elucidation of protein-protein interactions necessary for maintenance of the BCR-ABL signaling complex

  Tomas Gregor, et al., bioRxiv - Molecular Biology 2019

  Quote: ... Mouse FLAG (F1804; Sigma) and Goat V5 (sc-83849 ...
• Alpha synuclein aggresomes inhibit ciliogenesis and multiple functions of the centrosome

  Anila Iqbal, et al., bioRxiv - Neuroscience 2019

  Quote: ... mouse anti-vimentin (Sigma), 1μg/ml ...
• HIV-1 Vpr antagonizes innate immune activation by targeting karyopherin-mediated NF-κB/IRF3 nuclear transport

  Hataf Khan, et al., bioRxiv - Microbiology 2020

  Quote: ... Mouse-anti-flag (Sigma), Rabbit-anti-GFP (Abcam) ...
• HIV-1 Vpr antagonizes innate immune activation by targeting karyopherin-mediated NF-κB/IRF3 nuclear transport

  Hataf Khan, et al., bioRxiv - Microbiology 2020

  Quote: ... Mouse-anti-FLAG (Sigma), Rabbit-anti-HA (Sigma ...
• WWOX-Mediated Degradation of AMOTp130 Negatively Affects Egress of Filovirus VP40 VLPs

  Jingjing Liang, et al., bioRxiv - Microbiology 2021

  Quote: ... mouse anti-flag (Sigma), mouse anti-AMOT (Santa Cruz) ...
• Synaptogenic activity of the axon guidance molecule Robo2 is critical for hippocampal circuit function

  Heike Blockus, et al., bioRxiv - Neuroscience 2019

  Quote: ... mouse anti-Synaptophysin (Sigma), mouse anti-β-actin (Sigma) ...
• K_v1.3 induced hyperpolarisation and Ca_v3.2-mediated calcium entry are required for efficient Kaposi’s sarcoma-associated herpesvirus lytic replication

  Holli Carden, et al., bioRxiv - Microbiology 2021

  Quote: ... normal mouse IgG (Millipore). qPCR reactions were performed using either 2 μl of immunoprecipitated DNA or 2 μl of input DNA as template.
• Toxoplasma gondii AP2XII-2 contributes to proper progression through S-phase of the cell cycle

  Sandeep Srivastava, et al., bioRxiv - Microbiology 2020

  Quote: ... goat α-mouse (Sigma) 1:5,000 ...
• Cilium induction triggers differentiation of glioma stem cells

  Gladiola Goranci-Buzhala, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-FLAG (Sigma), rabbit anti-TUJ1 (Sigma) ...
• SERINC5 potently restricts retrovirus infection in vivo

  Uddhav Timilsina, et al., bioRxiv - Microbiology 2020

  Quote: ... -anti-mouse (EMD Millipore) and -anti-goat (Sigma Aldrich ...
• An extended DNA-free intranuclear compartment organizes centrosomal microtubules in Plasmodium falciparum

  Caroline S. Simon, et al., bioRxiv - Cell Biology 2021

  Quote: ... anti-mouse-Atto647 (Sigma) and anti-rat-Atto594 (Biomol ...
• The Autism Risk Factor CHD8 Is a Chromatin Activator in Human Neurons and Functionally Dependent on the ERK-MAPK Pathway Effector ELK1

  Bahareh Haddad Derafshi, et al., bioRxiv - Cell Biology 2021

  Quote: ... Map2 (Mouse-Sigma-M9942) used as 1:500,Tuj1 (Rabbit-Biolegened-802001 ...
• Impaired OTUD7A-dependent Ankyrin regulation mediates neuronal dysfunction in mouse and human models of the 15q13.3 microdeletion syndrome

  Brianna K. Unda, et al., bioRxiv - Neuroscience 2022

  Quote: ... Mouse anti-FLAG (Sigma F1804 ...
• Molecular characteristics and laminar distribution of prefrontal neurons projecting to the mesolimbic system

  Ákos Babiczky, Ferenc Mátyás, bioRxiv - Neuroscience 2022

  Quote: ... FoxP2 (mouse, Merck Millipore: MABE415 ...
• TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance

  Acong Yang, et al., bioRxiv - Molecular Biology 2022

  Quote: ... mouse anti-Flag (Sigma) and mouse anti-α-tubulin (Sigma) ...
• Premature MeCP2 Expression Disturbs the Subtype Specification of Midbrain Dopamine Neurons

  Xi-Biao He, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-TH (Sigma), rabbit anti-NURR1 (Santa Cruz Biotechnology).
• Neural precursor cells contribute to decision-making by tuning striatal connectivity via secretion of IGFBPL-1

  Erica Butti, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse anti NeuN (Millipore, product no ...
• Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling

  Jeffrey R. Johnson, et al., bioRxiv - Microbiology 2020

  Quote: ... mouse anti-GAPDH (Sigma) at 1:2000 ...
• A novel histone deacetylase inhibitor-based approach to eliminate microglia and retain astrocyte properties in glial cell culture

  Xi-Biao He, Yi Wu, Haozhi Huang, Fang Guo, bioRxiv - Neuroscience 2021

  Quote: ... mouse anti-GFAP (Sigma) and rabbit anti-Ki67 (Abcam).
• Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry

  Lance Hsieh, et al., bioRxiv - Cell Biology 2022

  Quote: ... SPTAN1 (MAB1662 mouse, Sigma) and k-Ras (AB108602 rabbit ...
• Snf1/AMPK fine-tunes TORC1 signaling in response to glucose starvation

  Marco Caligaris, et al., bioRxiv - Biochemistry 2022

  Quote: ... mouse anti-His6 (Sigma, product number H1029 ...
• Spt6 is a maintenance factor for centromeric CENP-A

  Georg OM Bobkov, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse α tubulin (Sigma) and mouse α V5 (Invitrogen) ...
• Cysteine residues in the C-terminal tail of connexin32 regulate its trafficking

  Anuttoma Ray, Parmender P. Mehta, bioRxiv - Cell Biology 2020

  Quote: ... anti-mouse beads (Sigma) was washed with Tris-NP40 buffer once ...
• Membrane Protrusion Formation Mediated by Rho/ROCK Signalling and Modulation of Chloride Flux

  Akiko Hori, et al., bioRxiv - Cell Biology 2019

  Quote: ... HA (mouse; SIGMA; #H9658). Chemicals were ...
• A novel effect of the antigenic peptides position for presentation upon MHC class I

  Jun Imai, et al., bioRxiv - Immunology 2019

  Quote: ... Anti-actin (mouse; Millipore), and 25-D1.16 were used in this study ...
• Centromeric CENP-A loading requires accurate mitotic timing, which is linked to checkpoint proteins

  Anne Laure Pauleau, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse tubulin (Sigma T9026), mouse V5 (Invitrogen V8012) ...
• Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage

  Sophie Colombo, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse against Gfap (Sigma C9205 & Cell Signalling Technology 3670 ...
• Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cells

  Simone Lieb, et al., bioRxiv - Cancer Biology 2019

  Quote: ... mouse anti-FLAG (SIGMA, F1804 ...
• Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking and synchronized extracellular vesicle release of CD9 and CD63

  Mathilde Mathieu, et al., bioRxiv - Cell Biology 2020

  Quote: ... normal mouse IgG (Millipore); rabbit anti-GFP polyclonal antibody was purchased from the Tab-IP antibody and the recombinant proteins facilities at the Institut Curie.
• Ndel1 promotes keratin assembly and desmosome stability

  Yong-Bae Kim, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-CD3 (Millipore), guinea pig anti-Dsc1 (gift from I ...