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Citations for Millipore Sigma :
1401 - 1450 of 5383 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 10 μg ml−1 insulin (I9278, Sigma-Aldrich, MO, USA), 0.2 μg ml−1 hydrocortisone (H0888 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.035% 2-mercaptoethanol and 1:4000 insulin (Sigma Aldrich, I9278) and after that time were cultured in suspension in 6 wells plates ...
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bioRxiv - Genetics 2024Quote: ... 10 μg/mL insulin (Sigma-Aldrich, St. Louis, MO, USA), and 3 U/mL heparin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... bovine insulin and Tween 20 were obtained from Sigma-Aldrich. Triton X-100 and paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2023Quote: ... and antibodies against insulin and avidin were from Sigma-Aldrich. Antibodies against the β subunit of the insulin receptor were from Santa Cruz Biotechnology ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 μg/mL insulin-transferrin-selenium (ITS, Sigma-Aldrich). Follicles were cultured for 6 days at 37 °C in a humidified environment of 5% CO2 in air ...
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bioRxiv - Neuroscience 2024Quote: ... β-mercaptoethanol (all from ThermoFisherScientific) and 5μg/ml insulin (Sigma), termed “N2B27” ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Neuroscience 2021Quote: ... and the GABA-B receptor agonist baclofen (Sigma Aldrich GmbH, Munich, Germany) were dissolved together in saline at a dose of 20ng/μl (muscimol ...
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bioRxiv - Immunology 2021Quote: ... Sera were pre-treated with receptor destroying enzyme (RDE) (Sigma-Aldrich, C8772) and two-fold serially diluted ...
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bioRxiv - Neuroscience 2019Quote: ... γ-aminobutyric acid B (GABAB) receptors with CGP5243 (25 µM; Sigma, Australia), phasic GABAA receptors with SR95531 (0.5 µM ...
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bioRxiv - Neuroscience 2020Quote: The GABAA receptor agonist muscimol hydrobromide (Sigma-Aldrich, St. Louis, MO, USA) and antagonist (+)-bicuculline (Selleck ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-Interferon-α/β Receptor Chain 2 Antibody (IFNA2, clone MAB1155; Millipore) and its corresponding isotype (Mouse IgG2a ...
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bioRxiv - Neuroscience 2020Quote: ... the pan-glutamate receptor antagonist kynurenic acid (Sigma, 40mM, 8.5-9 pH) was filled in one barrel of the glass electrode and microointophoretically applied to the recording site ...
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bioRxiv - Biochemistry 2021Quote: ... an angiotensin II receptor blocker (Arb; 10 μM for 24h; Sigma-Aldrich), or their combination for 24 h ...
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bioRxiv - Microbiology 2019Quote: ... or in response to muscarinic receptor stimulation with 100 µM carbachol (Sigma). The difference between basal Isc and peak Isc recorded after veratridine or carbachol addition was measured (ΔIsc ...
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bioRxiv - Cancer Biology 2021Quote: ... as well as GABA-A receptor antagonists like Bicuculline methbromide (Sigma #B7561) and Picrotoxin (Sigma #P1675) ...
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bioRxiv - Microbiology 2021Quote: ... Soluble receptor expression was induced by adding 1 μg/ml doxycycline (Sigma) to the cells the day before the assay ...
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bioRxiv - Molecular Biology 2020Quote: ... The GABAB receptor was concentrated in a 100-kDa cutoff Vivaspin (Millipore) filter and run on a Superose™ 6 Increase column (GE Healthcare).
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bioRxiv - Bioengineering 2020Quote: Ready-to-Assay Platelet Activating Factor Receptor Cells (Millipore Sigma, Burlington, MA) (Chem-1 host cells overexpressing PAFR GPCR ...
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bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-mu opioid receptor (MOR) antibody (1:1000; EMD Millipore: AB5511) was applied at 4°C in 1x PBS containing 0.25% Triton-X-100 (PBS-Tx ...
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bioRxiv - Cell Biology 2022Quote: ... RyR receptor (tetracaine) or gap junctions [carbennoxolone (CBX)] were from Sigma-Aldrich.
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bioRxiv - Microbiology 2022Quote: ... sera were treated with receptor-destroying enzyme (Sigma-Aldrich, St. Louis, MO), heat inactivated at 56°C ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-glutamate receptor 2 (mouse anti-GluR2 IgG2a; Millipore; 1:2000). Sections were then washed with PBS and incubated in Alexa Fluor-conjugated fluorescent secondary antibodies (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The blocking buffer for Estrogen receptor (EMD Millipore Cat#04820MI, 1:200) and Ki67 (Abcam Cat#ab16667 ...
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bioRxiv - Neuroscience 2023Quote: ... NMDA receptor antagonist AP-5 (2 mM; Sigma Aldrich, St. Louis, MO), and AMPA receptor antagonist CNQX (0.4 mM ...
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bioRxiv - Neuroscience 2023Quote: ... and AMPA receptor antagonist CNQX (0.4 mM; Sigma Aldrich, St. Louis, MO), or the selective GABA-A receptor agonist muscimol (100 ng ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... nicotinic acetylcholine receptor antagonist mecamylamine (5 mg kg−1,M9020, Sigma-Aldrich) and muscarinic acetylcholine receptor antagonist atropine (5 mg kg−1 ...
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bioRxiv - Neuroscience 2023Quote: ... The D2 receptor (D2R) antagonist haloperidol was also obtained from Sigma-Aldrich, prepared in a 0.9% NaCl solution ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we tested the broad opioid receptor antagonist (naltrexone hydrochloride (Millipore-Sigma, #N3136)) injected ip at the dosage of 10 ug/uL (aggression N = 10 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we tested the broad opioid receptor antagonist (naltrexone hydrochloride (Millipore-Sigma, #N3136)) injected ip at the dosage of 10 ug/uL (aggression N = 10 ...
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bioRxiv - Neuroscience 2023Quote: ... Rat Anti-Dopamine D1 Receptor (1:200, Sigma, Cat# D2944, RRID: AB_1840787), Rabbit Anti-Dopamine D2 Receptor (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used included rabbit anti-P2RY12 (Purinergic Receptor P2Y12; Sigma Prestige Antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... to block NMDA receptors and 100 μM picrotoxin (PTX; P1675 Sigma-Aldrich) to block GABAA receptors ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µM strychnine hydrochloride (glycine receptor antagonist, Sigma-Aldrich, Cat #: S8753). Drugs were bath applied for at least 5 – 10 minutes before responses were measured.
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.25x Protease Inhibitor Cocktail for Histidine-tagged proteins (Sigma-Aldrich), and 0.3 mg/mL lysozyme ...
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µl protease inhibitor cocktail for histidine tagged proteins (Sigma) / 0.2 g weight of E ...
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bioRxiv - Plant Biology 2023Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST tag (Step 2 ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Immunology 2021Quote: ... ELISpot plates were coated with native Insulin (Sigma-Aldrich, Cat. 91077C), anti-IgM (Mabtech ...