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Citations for Millipore Sigma :
1401 - 1450 of 2558 citations for PTH Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2021Quote: ... Immunoprecipitated proteins were eluted overnight at 4 °C with 50 μg of Flag peptide (Sigma-Aldrich) in 100 μL complemented non-denaturing lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... The trypsinized peptides were vacuum-desiccated and solubilized in 0.1% trifluoroacetic acid (TFA; 299537, Sigma-Aldrich). The sample was then desalted using C18 Ziptip (ZTC18S960 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was eluted twice from resin using 100μL of 500ng/μL [3x flag peptide (Sigma F4799), Myc (Pierce 20170) ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...
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bioRxiv - Plant Biology 2020Quote: ... Bound effectors and associated plant proteins were competitively eluted by 3XFLAG peptides (Sigma-Aldrich, Dorsett, England) and separated by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... The retained complexes were eluted twice with either 3x FLAG peptide (200 μg/ml Sigma-Aldrich), HA peptide (400 μg/ml Roche ...
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bioRxiv - Systems Biology 2021Quote: ... 2 pmol (2 µL of stock) of the non-labelled AQUA® peptide HLEAAKGYSFTTTAEKAAELHK (Sigma-Aldrich) containing the quantification tag sequence GYSFTTTAEK was added to enable quantification of SIL-protein stock concentrations using MS analysis based on the ratio of heavy-to-light GYSFTTTAEK signals (see below) ...
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bioRxiv - Molecular Biology 2020Quote: ... One milligram of peptide was dissolved in 20 ul of 10% trifluoroacetic acid (TFA, Sigma-Aldrich), in an H218O base (95-98% 18O ...
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bioRxiv - Microbiology 2020Quote: ... Dried peptides were resuspended in a loading buffer consisting of 20 mM citric acid (Sigma-Aldrich) and 1% formic acid (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylated peptides were acidified with 1 μL of trifluoroacetic acid (TFA) (Fluka BioChemika, Sigma-Aldrich, GmbH) to inhibit trypsin and acidify peptides for desalting using C-18 StageTips ...
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bioRxiv - Biophysics 2020Quote: ... N-terminally labelled PR25 was obtained by reacting the peptide with amine-reactive AlexaFluor546 (Sigma-Aldrich). PolyU RNA with a molecular weight range from 800– 1,000 kDa was purchased from Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Biochemistry 2022Quote: ... The resin was incubated with 1 mL of 0.5 mg/mL 3x FLAG peptide (Sigma-Aldrich) in B-0.1 and rotated at 4°C for 1 h to elute the tagged remodeler ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides extracted from trypsin digestion were purified with ZipTip C18 pipette tips (Millipore, Billerica, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The protein complexes were specifically eluted with 125 mkl of 100 ng/mkl 3xFLAG peptide (Sigma) solution in TBS during 30 min on-ice incubation with agitation ...
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bioRxiv - Synthetic Biology 2022Quote: ... the synthesized peptide microchip was incubated with 100 ug/ml guinea pig liver tissue transglutaminase (Sigma) and 3 mM dansyl-cadaverine (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... bound proteins were eluted using 100 μL of FLAG peptide (100 μg/mL, Sigma-Aldrich, F4799), and then with 100 μL of 2x Laemli buffer (62.5 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Absolute quantification of each protein is performed through applying AQUA™ Peptides purchased from Sigma-Aldrich. A summary of all quantification results were shown in S8 Table.
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bioRxiv - Cancer Biology 2020Quote: ... Anti-FLAG M2 affinity gel (A2220) and 3 x FLAG peptide (F3290) were from Sigma-Aldrich, protein A or G beads were from Invitrogen (10004D) ...
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bioRxiv - Immunology 2020Quote: ... Highly hydrophobic peptides were dissolved in 10 to 50% (vol/vol) dimethyl sulfoxide (DMSO) (Sigma D2650).
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 peptide was purified under denaturing conditions using Novagen NiNTA purification resins (Sigma, Catalog #: 70666) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... tryptic peptides were acidified to pH < 2 by adding 10 μL of 10% TFA (Sigma, 1002641000).
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bioRxiv - Immunology 2021Quote: ... The CXCR4-derived peptide msR4M-L1 was freshly dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) (Sigma-Aldrich) at 4°C at a concentration of 1 or 2 mM ...
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bioRxiv - Genetics 2019Quote: ... The peptides were extracted from the beads by adding 50 µL of 5% Formic acid (Sigma). The extraction was repeated one more time and the eluted peptides were combined ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... Two sequential elutions were carried out with 100 μl 0.1 mg/ml 3× Flag peptide (Sigma) in RNC buffer at room temperature for 25 min ...
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bioRxiv - Neuroscience 2023Quote: ... or Heparinase 1 + autocamtide-2-related inhibitory peptide (AIP, 0.17 μg/μl/site, Sigma-Aldrich, SCP0001) was injected into hippocampal CA1 area at a rate of 3 nl/s ...
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bioRxiv - Plant Biology 2022Quote: ... Captured proteins were eluted off FLAG beads using 500 μg/mL 3x-FLAG peptide (Sigma-Aldrich). Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... bound proteins were eluted using buffer A containing 500ng/μL 3X FLAG® peptide (Sigma; F4799) for followed SDS-PAGE.
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bioRxiv - Neuroscience 2023Quote: ... Peptides were acidified and loaded onto p200 pipette tips packed with Empore SPE disks C18 (Sigma) and Oligo R3 Resin (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... we pre-incubated it with the GnRH2 and GnRH3 peptides (SCP0183 and L4897 respectively, Sigma-Aldrich) at a concentration of 100 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and ZmRALF12 coding sequences24 without signal peptides were amplified and cloned into the pET-32a (Novagen) vector ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Microbiology 2024Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, St. Louis, MO) and separated with a 10 min LC gradient (10% Buffer A (0.1% formic acid (FA ...
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bioRxiv - Cell Biology 2024Quote: ... and the peptide mixtures were desalted on C-18 reverse phase material (ZipTip μ-C18, Millipore). Eluted peptides were concentrated by evaporation under vacuum and subsequently resolved in 0.1% acetic acid / 2% acetonitrile containing HRM/iRT peptides (Biognosys ...
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bioRxiv - Systems Biology 2023Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, St. Louis, MO) and separated with a 10 minute LC gradient (10% Buffer A (0.1% FA in water ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoprecipitated proteins were eluted from Dynabeads by incubating with 200 μM FLAG peptides (Sigma-Aldrich) for 90 min ...
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bioRxiv - Microbiology 2023Quote: ... Bound protein was eluted in 30 μl of elution buffer (0.15 mg/ml 3xFLAG peptide [Sigma, St Louis ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Microbiology 2023Quote: ... the immunoprecipitated fraction was eluted with 40 µL of 0.1 mg/mL 3xFlag peptide (Sigma-Aldrich) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The groups consisted of the 16 synthesized vaccine peptides with poly(I:C) (Sigma-Aldrich cat. #P1530), 16 synthesized vaccine peptides with STING agonist BI-1387446 ...
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bioRxiv - Biophysics 2023Quote: ... All other reagents applied in peptide synthesis were purchased from Sigma Aldrich(St. Louis, MO, USA). Sodium dihydrogen phosphate ...
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bioRxiv - Biophysics 2023Quote: ... peptides with the sequences SVEGEGEEEGEEY (α-CTT) and DATAEEEEDFGEEAEEEA (β-CTT) were purchased from Sigma-Aldrich and used without further purification ...
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bioRxiv - Biochemistry 2023Quote: ... 0.01/0.001% LMNG/CHS and eluted by adding 250 µg/ml 3xFLAG peptide (final concentration, Sigma). The total supernatant was analyzed via 10% SDS-PAGE ...
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bioRxiv - Bioengineering 2022Quote: ... calpain substrate peptide or conjugates were incubated with 26.6 nM human calpain-1 (Sigma-Aldrich, C6108) in 50 mM N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid (HEPES) ...
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bioRxiv - Evolutionary Biology 2023Quote: (Bio-Techne: https://www.bio-techne.com/p/small-molecules-peptides/dtag-13_6605) was reconstituted in DMSO (Sigma) to a final concentration of 5 mM ...
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bioRxiv - Cell Biology 2022Quote: ... without any antibodies or the immunoprecipitation was outcompeted with the 3XFLAG peptide (340 μm; Sigma, F4799). Beads were then washed 6 times with Buffer A with 0.1% Triton X-100 followed by elution with Laemmli buffer ...